LOCUS       sequence-344807-        6098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses jGCaMP7c variant 1513 in astrocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Chneiweiss, H
  TITLE     Chneiweiss Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-GFAP-jGCaMP7c variant 1513
FEATURES             Location/Qualifiers
     source          1..6098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      150..605
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(237..256)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     447..468
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(622..641)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     741..763
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(801..819)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        887..991
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(992..1060,1061..1852)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2023..2611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2512..2531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   2673..2802
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      3519..3528
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3525..3533,3534..3551,3552..3554,3555..3587,3588..3590,
                     3591..3614,3615..3641,3642..3698,3699..3701,3702..3704,
                     3705..3974,3975..3992,3993..4427,4428..4433,4434..4877)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6m
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSQPNVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKGSYRDSEEEIREAFGVFDKDGN
                     GYISAAELRHVMNNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      3621..3630
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     3911..3932
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4038..4059)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4299..4318)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    4897..5485
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4950..4970)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5368..5379)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5517..5993
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5716..5735)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   join(6033..6098,1..75)
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   join(6033..6098,1..64)
                     /label=AAV2 ITR
ORIGIN
        1 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
       61 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
      121 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
      181 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc
      241 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
      301 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
      361 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
      421 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
      481 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
      541 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
      601 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
      661 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
      721 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
      781 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
      841 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      901 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      961 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     1021 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     1081 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     1141 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     1201 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     1261 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     1321 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     1381 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     1441 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     1501 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     1561 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     1621 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     1681 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     1741 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1801 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     1861 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1921 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1981 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2041 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2101 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2161 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2221 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2281 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2341 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2401 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2461 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2521 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2581 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2641 cctggccttt tgctggcctt ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct
     2701 cactgaggcc gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag
     2761 cgcgcagaga gggagtggcc aactccatca ctaggggttc ctgcggccgc acgcgtgatc
     2821 taacatatcc tggtgtggag taggggacgc tgctctgaca gaggctcggg ggcctgagct
     2881 ggctctgtga gctggggagg aggcagacag ccaggccttg tctgcaagca gacctggcag
     2941 cattgggctg gccgcccccc agggcctcct cttcatgccc agtgaatgac tcaccttggc
     3001 acagacacaa tgttcggggt gggcacagtg cctgcttccc gccgcacccc agcccccctc
     3061 aaatgccttc cgagaagccc attgagcagg gggcttgcat tgcaccccag cctgacagcc
     3121 tggcatcttg ggataaaagc agcacagccc cctaggggct gcccttgctg tgtggcgcca
     3181 ccggcggtgg agaacaaggc tctattcagc ctgtgcccag gaaaggggat caggggatgc
     3241 ccaggcatgg acagtgggtg gcaggggggg agaggagggc tgtctgcttc ccagaagtcc
     3301 aaggacacaa atgggtgagg ggagagctct ccccatagct gggctgcggc ccaaccccac
     3361 cccctcaggc tatgccaggg ggtgttgcca ggggcacccg ggcatcgcca gtctagccca
     3421 ctccttcata aagccctcgc atcccaggag cgagcagagc cagagcaggt tggagaggag
     3481 acgcatcacc tccgctgctc gcggggtcta gagtcgacgc caccatgggt tctcatcatc
     3541 atcatcatca tggtatggct agcatgactg gtggacagca aatgggtcgg gatctgtacg
     3601 acgatgacga taaggatctc gccaccatgg tcgactcatc acgtcgtaag tggaataaga
     3661 caggtcacgc agtcagagct ataggtcggc tgagctcaca gcctaacgtc tatatcaagg
     3721 ccgacaagca gaagaacggc atcaaggcga acttcaagat ccgccacaac atcgaggacg
     3781 gcggcgtgca gctcgcctac cactaccagc agaacacccc catcggcgac ggccccgtgc
     3841 tgctgcccga caaccactac ctgagcgtgc agtccaaact ttcgaaagac cccaacgaga
     3901 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     3961 acgagctgta caagggcggt accggaggga gcatggtgag caagggcgag gagctgttca
     4021 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     4081 tgtccggcga gggtgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     4141 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     4201 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     4261 ccgaaggcta catccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     4321 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     4381 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacctt cctgaccaac
     4441 tgactgaaga gcagatcgca gaatttaaag aggctttctc cctatttgac aaggacgggg
     4501 atgggacaat aacaaccaag gagctgggga cggtgatgcg ttctctgggg cagaacccca
     4561 cagaagcaga gctgcaggac atgatcaatg aagtagatgc cgacggtgac ggcacaatcg
     4621 acttccctga gttcctgaca atgatggcaa gaaaagggag ctacagggac agtgaagaag
     4681 aaattagaga agcgttcggt gtgtttgata aggatggcaa tggctacatc agtgcagcag
     4741 agcttcgcca cgtgatgaac aaccttggag agaagttaac agatgaagag gttgatgaaa
     4801 tgatcaggga agcagacatc gatggggatg gtcaggtaaa ctacgaagag tttgtacaaa
     4861 tgatgacagc gaagtgacga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     4921 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     4981 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5041 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5101 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5161 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5221 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     5281 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct atgttgccac ctggattctg
     5341 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     5401 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     5461 atctcccttt gggccgcctc cccgcatcga taccgagcgc tgctcgagag atctacgggt
     5521 ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc
     5581 ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct
     5641 ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct
     5701 gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca
     5761 ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg
     5821 gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt
     5881 tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc
     5941 ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt
     6001 tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac
     6061 tccctctctg cgcgctcgct cgctcactga ggccgggc
//
