LOCUS       sequence-34483-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  TITLE     Barton Lab Unpublished Plasmids
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Flag-TRIM24-F979A/N980A shRescue
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             122..145
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttgccaccat ggcagactac aaggacgacg atgacaaggc
      121 agactacaag gacgacgatg acaagggtac ctctagaaga tctaggacaa tggaggtggc
      181 ggtggagaag gcggtggcgg cggcggcagc ggcctcggct gcggcctccg gggggccctc
      241 ggcggcgccg agcggggaga acgaggccga gagtcggcag ggcccggact cggagcgcgg
      301 cggcgaggcg gcccggctca acctgttgga cacttgcgcc gtgtgccacc agaacatcca
      361 gagccgggcg cccaagctgc tgccctgcct gcactctttc tgccagcgct gcctgcccgc
      421 gccccagcgc tacctcatgc tgcccgcgcc catgctgggc tcggccgaga ccccgccacc
      481 cgtccctgcc cccggctcgc cggtcagcgg ctcgtcgccg ttcgccaccc aagttggagt
      541 cattcgttgc ccagtttgca gccaagaatg tgcagagaga cacatcatag ataacttttt
      601 tgtgaaggac actactgagg ttcccagcag tacagtagaa aagtcaaatc aggtatgtac
      661 aagctgtgag gacaacgcag aagccaatgg gttttgtgta gagtgtgttg aatggctctg
      721 caagacgtgt atcagagctc atcagagggt aaagt
//
