LOCUS       sequence-344857-        9717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a sfGFP dropout expression cassette in place of Part 2 of 
            HexaPro-D614G spike to be used in the Spike Display system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9717)
  AUTHORS   Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, 
            Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, 
            Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ
  TITLE     Rapid characterization of spike variants via mammalian cell surface 
            display.
  JOURNAL   Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 
            10.1016/j.molcel.2021.11.024.
  PUBMED    34919820
REFERENCE   2  (bases 1 to 9717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.11"; journalName: "Mol Cell"; date: 
            "2021-12-16- 16"; volume: "81"; issue: "24"; pages: "5099-5111.e8"
COMMENT     Sequence Label: Spike Display_Part 2 DO
FEATURES             Location/Qualifiers
     source          1..9717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      692..763
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      779..806
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             2981..3046
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3053..3076
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3083..3106
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3149..3295
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(3342..3359)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3348..3572
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3705..3724)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    3856..3903
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             3911..4933
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             3911..4933
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5063..5184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5100..5119)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5154..5173
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5233..5249)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5233..5249)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5246..5268)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5257..5273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5281..5287,5288..5305,5306..5311))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5326..5347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5464..5481)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5635..6223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5715..5734)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6394..7185,7186..7254))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7017..7036
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7255..7358)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7434..7453)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7625..8004
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8005..8208
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8159..8179
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    8210..8228
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8231..8249
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     8259..8283
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          8402..8534
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     8517..8536
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        8579..8597
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     8643..8702
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     RBS             9666..9677
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(9684..9717,1..680)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 tccctattct ggtggaactg gatggtgatg tcaacggtca taagttttcc gtgcgtggcg
       61 agggtgaagg tgacgcaact aatggtaaac tgacgctgaa gttcatctgt actactggta
      121 aactgccggt tccttggccg actctggtaa cgacgctgac ttatggtgtt cagtgctttg
      181 ctcgttatcc ggaccatatg aagcagcatg acttcttcaa gtccgccatg ccggaaggct
      241 atgtgcagga acgcacgatt tcctttaagg atgacggcac gtacaaaacg cgtgcggaag
      301 tgaaatttga aggcgatacc ctggtaaacc gcattgagct gaaaggcatt gactttaaag
      361 aggacggcaa tatcctgggc cataagctgg aatacaattt taacagccac aatgtttaca
      421 tcaccgccga taaacaaaaa aatggcatta aagcgaattt taaaattcgc cacaacgtgg
      481 aggatggcag cgtgcagctg gctgatcact accagcaaaa cactccaatc ggtgatggtc
      541 ctgttctgct gccagacaat cactatctga gcacgcaaag cgttctgtct aaagatccga
      601 acgagaaacg cgatcatatg gttctgctgg agttcgtaac cgcagcgggc atcacgcatg
      661 gtatggatga actgtacaaa tgaccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      721 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      781 ctcaccttcg ggtgggcctt tctgcgttta tacacctgcc accgccaccg tctgcggtcc
      841 taagaagtcc accaatctgg ttaagaacaa atgcgtgaac ttcaacttca acggcctgac
      901 cgggaccggc gtgctgaccg aaagcaacaa aaagttcctc cccttccagc agttcggccg
      961 tgatatcgct gacaccacag atgccgtcag agatccacag accctggaaa tcctggatat
     1021 tacaccctgc tccttcggag gagtttctgt gatcaccccc gggaccaata ccagcaacca
     1081 ggtggctgtg ctgtaccaag gtgttaactg caccgaggtt cctgtggcca tccacgccga
     1141 tcagctgaca cctacttgga gagtgtactc cactggctcc aatgtgttcc agaccagggc
     1201 cggatgtctg atcggcgccg agcacgtgaa taacagttac gagtgcgaca tccctatcgg
     1261 cgccggcatc tgtgccagct accagaccca gacaaacagc cctgggtctg cttcctctgt
     1321 agctagccag agcatcatcg cctacaccat gagcctgggc gcagagaaca gcgtggccta
     1381 ttccaacaac tctatcgcca ttcccaccaa ctttacaatt agcgtcacaa cagagatcct
     1441 gcccgtgagc atgaccaaga ccagcgtgga ctgtacaatg tacatctgtg gcgacagcac
     1501 tgaatgcagc aacctgctgc tgcaatacgg ctccttttgc acccaactga accgggcgct
     1561 gaccggaatc gccgtggaac aggacaaaaa tacccaggag gtgttcgccc aagtgaagca
     1621 gatctacaag accccaccta tcaaggactt cggcggcttt aactttagcc agattctccc
     1681 tgatccttct aagcctagca agcggagccc tatcgaggat ctgctgttca acaaggtcac
     1741 cctggccgat gccggcttta tcaaacagta tggcgattgc ctgggcgaca tagccgccag
     1801 agatctgatc tgcgcccaga aattcaacgg cctgacagtt ctcccacctc tgctgaccga
     1861 cgagatgatc gctcagtaca cctctgccct gctggctggc accatcacat ctgggtggac
     1921 atttggcgcc ggccccgccc tgcagatccc ctttcccatg cagatggcct atagattcaa
     1981 cggaatcggc gtgacccaga acgtgctgta tgaaaaccag aagctgatcg ctaaccagtt
     2041 caattctgcc atcggcaaga tccaggactc cctctcctct acccccagcg ccctgggcaa
     2101 actgcaggac gtggtgaatc agaacgccca agccctgaac accctggtga agcagctcag
     2161 cagcaatttt ggcgccatca gctctgtgct gaacgatatc ctgtctagac tggaccctcc
     2221 agaagccgaa gtccagatcg atagactgat cacaggcaga ctgcagtccc tgcaaaccta
     2281 cgtgacccaa cagctgatca gggccgctga aataagagcc agcgccaatc tcgccgctac
     2341 caagatgtcc gagtgtgtgc tgggacagtc taaacgcgtt gacttctgcg gcaaaggcta
     2401 tcacctgatg agcttccccc agagcgcgcc gcacggcgtg gtgttcctgc atgtgacata
     2461 cgtgcctgcc caagagaaga atttcacaac cgcccctgcc atctgccacg acggcaaggc
     2521 ccacttccca agagagggcg ttttcgtttc caatggcaca cactggttcg tgacacaaag
     2581 aaacttctac gaaccccaga ttatcaccac cgacaacacc ttcgtgagtg gcaattgtga
     2641 cgtggtcatc ggaatcgtga acaacacagt gtacgaccct ctgcaacctg agctggactc
     2701 ttttaaggaa gagctggaca agtactttaa aaaccacacc agccccgatg tggacctggg
     2761 cgacatcagt ggcattaacg ccagcgtggt gaacatccaa aaggaaatcg acagactgaa
     2821 cgaggtggcc aagaacctga acgagtccct gatcgacctg caggagctcg gcaaatacga
     2881 gcagggatcc ggatacatcc ccgaggcccc cagagatggc caggcctacg tgcggaagga
     2941 cggcgagtgg gtactgctga gcacattcct gggcagatcc gactacaaag accatgacgg
     3001 tgattataaa gatcatgaca tcgattacaa ggatgacgat gacaagagcg cctggtccca
     3061 cccccagttc gagaagggat ccctggaggt gctgttccag ggcccaggcg gcggcggtag
     3121 tggagggggc ggatctggcg gctcagctgc tgtgggccag gacacgcagg aggtcatcgt
     3181 ggtgccacac tccttgccct ttaaggtggt ggtgatctca gccatcctgg ccctggtggt
     3241 gctcaccatc atctccctta tcatcctcat catgctttgg cagaagaagc cacgttaacg
     3301 gccgctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     3361 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     3421 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     3481 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     3541 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     3601 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3661 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     3721 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3781 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     3841 tggttcacgt acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     3901 ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     3961 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     4021 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     4081 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     4141 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     4201 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     4261 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     4321 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     4381 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     4441 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     4501 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     4561 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     4621 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
     4681 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     4741 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     4801 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     4861 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
     4921 ggcaaaggaa tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt
     4981 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     5041 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     5101 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5161 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     5221 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5281 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5341 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5401 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5461 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5521 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5581 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5641 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcgga ggtggcgaaa
     5701 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5761 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5821 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5881 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5941 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6001 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6061 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6121 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6181 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6241 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6301 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6361 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6421 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6481 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6541 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6601 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6661 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6721 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6781 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6841 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6901 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6961 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7021 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7081 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7141 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7201 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7261 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7321 tgaatgtatt tagaaaaata aacaaatagg ggttccgcac acatttcccc gaaaagtgcc
     7381 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     7441 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     7501 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     7561 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     7621 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     7681 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     7741 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     7801 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     7861 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     7921 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     7981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     8041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     8101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     8161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt
     8221 gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag
     8281 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
     8341 agcctccgga ctctagcgtt taaacttaag ctgcagaagt tggtcgtgag gcactgggca
     8401 ggtaagtatc aaggttacaa gacaggttta aggagaccaa tagaaactgg gcttgtcgag
     8461 acagagaaga ctcttgcgtt tctgataggc acctattggt cttactgaca tccactttgc
     8521 ctttctctcc acaggtgtcc actcccagtt caattacagc tcttaaggct agagtactta
     8581 atacgactca ctataggcta gcctcgagaa ttcacgcgtg gtacctctag agtcgacccg
     8641 ggatggagac agacacactc ctgctatggg tactgctgct ctgggttcca ggttccactg
     8701 gtgacatgtt cgtgttcctg gtgctcctgc ctctggtgag cagccagtgc gtgaacctga
     8761 ccacccgaac ccagctccca ccagcctaca ccaacagctt tacacggggc gtgtactacc
     8821 ctgacaaggt gttcagatct agcgtcctgc acagcactca ggacctcttc ctgccgttct
     8881 tcagcaacgt gacatggttc cacgccatcc acgtgagcgg cacaaacgga accaagcggt
     8941 ttgataaccc cgtcctgcca ttcaatgatg gagtttactt cgccagtacc gagaagagta
     9001 acatcatccg gggctggatc ttcggcacca ccctggatag caaaacacag agcctcctga
     9061 tcgtgaacaa tgccacgaac gtcgtgatca aggtgtgcga gttccagttt tgcaatgatc
     9121 ctttcctggg tgtgtactac cacaagaaca acaagagctg gatggaaagc gagttcagag
     9181 tctacagcag cgccaacaac tgcacattcg agtacgtctc tcagcctttt ctgatggacc
     9241 ttgaggggaa acaaggcaac ttcaagaacc tgagagaatt cgtgttcaag aacatcgacg
     9301 gctacttcaa aatctactcc aagcacacac ccatcaacct ggtccgggac ctccctcagg
     9361 gcttcagcgc cctggaaccc ctggtcgacc tgcccatagg catcaacata acgcggttcc
     9421 aaaccctgct ggccctgcat agatcctacc tgactcctgg cgacagcagc agcggatgga
     9481 ccgccggagc tgcagcctac tatgtgggtg gcaggtggaa agtgaaacgt gatttcatgc
     9541 gtcattttga acattttgta aatcttattt aataatgtgt gcggcaattc acatttaatt
     9601 tatgaatgtt ttcttaacat cgcggcaact caagaaacgg caggttcgga tcttagctac
     9661 tagagaaaga ggagaaatac tagatgcgta aaggcgaaga gctgttcact ggtgtcg
//
