LOCUS       sequence-34486-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  TITLE     Barton Lab Unpublished Plasmids
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Flag-TRIM24-C840W/F869D/F979A/N980A
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      80..89
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             92..115
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             119..142
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagcttg ccaccatggc agactacaag gacgacgatg acaaggcaga
      121 ctacaaggac gacgatgaca agggtacctc tagaagatct aggacaatgg aggtggcggt
      181 ggagaaggcg gtggcggcgg cggcagcggc ctcggctgcg gcctccgggg ggccctcggc
      241 ggcgccgagc ggggagaacg aggccgagag tcggcagggc ccggactcgg agcgcggcgg
      301 cgaggcggcc cggctcaacc tgttggacac ttgcgccgtg tgccaccaga acatccagag
      361 ccgggcgccc aagctgctgc cctgcctgca ctctttctgc cagcgctgcc tgcccgcgcc
      421 ccagcgctac ctcatgctgc ccgcgcccat gctgggctcg gccgagaccc cgccacccgt
      481 ccctgccccc ggctcgccgg tcagcggctc gtcgccgttc gccacccaag ttggagtcat
      541 tcgttgccca gtttgcagcc aagaatgtgc agagagacac atcatagata acttttttgt
      601 gaaggacact actgaggttc ccagcagtac agtagaaaag tcaaatcagg tatgtacaag
      661 ctgtgaggac aacgcagaag ccaatgggtt ttgtgtagag tgtgttgaat ggctctgcaa
      721 gacgtgtatc agagctcatc agagggtaaa gttcacaa
//
