LOCUS       sequence-344879-       10038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2-plasmid ubiquitously expressing iCre (ubiquitin promoter) along
            with eGFP selection marker (Crystallin promoter -Lens/Eyes-).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Tromp A, Wang H, Hall TE, Mowry B, Giacomotto J
  TITLE     Optimising the zebrafish Cre/Lox toolbox. Codon improved iCre, new 
            gateway tools, Cre protein and guidelines.
  JOURNAL   Front Physiol. 2023 Aug 2;14:1221310. doi: 
            10.3389/fphys.2023.1221310. eCollection 2023.
  PUBMED    37601640
REFERENCE   2  (bases 1 to 10038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Physiol."; date: "2023-08-2"; volume: "14:1221310. doi"; pages: " 
            10.3389/fphys.2023.1221310. eCollection 2023"
COMMENT     Sequence Label: 114_UBI_iCre_crystGFP
FEATURES             Location/Qualifiers
     source          1..10038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      270..279
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(276..278,279..281,282..995)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(276..278,279..281,282..992)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(321..342)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(582..601)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     929..950
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1118..1239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(1402..1589,1590..1601)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(1613..1632)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1614..1632)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1639..1656)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1639..1655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1648..1670)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1796..2251
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1883..1902)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2093..2114
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2329..2433
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2434..2502,2503..3294)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2652..2671)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3465..4053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3954..3973
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4207..4224
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4341..4362
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4377..4382,4383..4400,4401..4407)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4415..4431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4420..4442
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4439..4455
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4439..4455
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        4473..4491
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4473..4490
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    join(4542..4553,4554..4691)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    4724..4744
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    8279..8303
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      8306..8315
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8318..8338
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(9369..9393)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    9409..9543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aggtcagagt ccaccccgct gattctgctg acaacactgt tcccaccatg agataatgcc
       61 attccagaga gatccatttg taagcccctc tttctgcagc acaggtatat aaccaggggt
      121 ctgcctccac taaggccggc acacatcatt tggggatctt tgtactgtac tagcctctat
      181 tagcctccta cttgcgttca gttcacatct tttgttcatc aggtgcaata agttacagta
      241 aggagttacc aggtctgaca ttaccggtcg ccaccatggt gagcaagggc gaggagctgt
      301 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      361 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      421 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
      481 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      541 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      601 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      661 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      721 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
      781 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
      841 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
      901 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
      961 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct agatcataat
     1021 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     1081 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     1141 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     1201 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggccaatctg gcctgtgttt
     1261 cagacaccag ggagtctctg ctcacgtttc ctgctatttg cagcctctct atcaagacta
     1321 atacacctct tcccgcatcg gctgcctgtg agaggccgcg gttagatcta ttaccctgtt
     1381 atccctaggg aaaatagaat gaagtgatct ccaaaaaata agtacttttt gactgtaaat
     1441 aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa atgtaattaa gtaaaagtaa
     1501 aagtattgat ttttaattgt actcaagtaa agtaaaaatc cccaaaaata atacttaagt
     1561 acagtaatca agtaaaatta ctcaagtact ttacacctct gggcccaatt cgccctatag
     1621 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     1681 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     1741 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg
     1801 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     1861 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     1921 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     1981 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     2041 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     2101 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     2161 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     2221 aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc
     2281 tgtgcggtat ttcacaccgc atcaggtggc acttttcggg gaaatgtgcg cggaacccct
     2341 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     2401 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     2461 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     2521 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     2581 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     2641 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     2701 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     2761 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     2821 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     2881 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     2941 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     3001 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     3061 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     3121 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     3181 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     3241 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     3301 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     3361 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     3421 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3481 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3541 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3601 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3661 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3721 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3781 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3841 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3901 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3961 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4021 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4081 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4141 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4201 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     4261 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     4321 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     4381 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     4441 ggaaacagct atgaccatga ttacgccaag ctatttaggt gacactatag aatactcaag
     4501 ctatgcatcc aacgcgttgg gagctctccc atatggtcga gcagaggtgt aaaaagtact
     4561 caaaaatttt actcaagtga aagtacaagt acttagggaa aattttactc aattaaaagt
     4621 aaaagtatct ggctagaatc ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt
     4681 acttgagtat taaggaagta gggataacag ggtaatctcg aatcaacttt gtatagaaaa
     4741 gttggctccg aattcgccct taaaactcga gaccagcaaa gttctagaat ttgtcgaaac
     4801 atttatgtta tatatttcct gaaaaaaatt ctgagtaagt tcttaagtgt tattgccagc
     4861 aacataaaca acagacggca aaatgaataa atgataacaa agcagtaggc ttaaataaac
     4921 ctaattttta taggctgttc tctacaaccc tcaaacagtg attagttttg tacttataaa
     4981 cttgcccttt cattcatatt tcaagaaaat tggttcagaa gatctggata ttctagcagt
     5041 tgttcaagct catggaggga tcagtgacct gattccacaa tgactaggcc taatccagaa
     5101 attagatgac tgtcaacata aaaaggcaca gcactcacta gctgccctat atattttatt
     5161 atattttaca tatattattt tatttattta gctctgagtg ctgtactttc tggttaaaga
     5221 aaactgctta caacagctaa cctgtactac ctcaggctca gggaatttgg aacaggtttg
     5281 tctggtttgt ttctttaacc atgcatgctt gttttcaact atggcaacac agtcacatgg
     5341 gacattacag aaatgatttg tcgatgacat gcgacttttc tttaataaag cgcaaagatc
     5401 ccaaaaagca aacttttaac aaaaatcata taattatatt ttcaatccag ctttgtagca
     5461 actttgtgct gctgttcact cagcaacaga tagtcagtat aaggtcagtg tgtctcaaag
     5521 cagtgccatc tgtttcacac attgcgttct atatataagt gtgctggttg acacgacact
     5581 gtataaggcc taggctaaaa cacaaacaat gtagaatgac actgtgtttt ttttgtaaac
     5641 aaatgttgtt tttggttaaa catctttgtg aaaacatcct cctgtcatgt atttgctata
     5701 ttcaaatgtt aaacccgtgc agaatagaac atatacaaaa aaaaacaaca caacacattt
     5761 ttaaacatta ttaaatatca agtattgctg gcagttctgt ttctgtttta cagtaccctt
     5821 tgccacagtt ctccgctttt cctggtccag attccacaag tctgattcac caatagcaaa
     5881 gcgaataaac aaccaaagca gccaatcact gcttgtagac tgtcctgcga gaccggccca
     5941 ttccagcaca ttctggaaac ttcctttata tgataattat aaatacattt aaattattga
     6001 tacaaaacat gtaattccta gaacataacc atagcaatca ttagttttca gggtaattat
     6061 gtatttttag gatttgactg cggaaagatc tggtcatgtg acgtctcatg aacgtcacgg
     6121 ccctgggttt ctataaatac agtaggactc tcgaccatcg gcagattttt cgaagaagaa
     6181 gatcagtttc aggagccgta ctgttccgtt tcaacgcaaa tattaacggt aagagcgaat
     6241 ttcctagttt gttttcatgc cattctttaa aaccatagcg tattacttta attatagtaa
     6301 actttcgctt tctttattac aagagacgtt ttgtgttgat tctccgcgga cattttcggt
     6361 cagacaatca gaaaatgacc gcggaggacc agtaacttgc attacacgta agttaaatct
     6421 tcgtgtatta aaatggttag gttgttaacg tcaaataggt taccgtgttt gcgtgtgatc
     6481 aggttggttt tgttagattt ttgtcagtat ttttaattta tttgttttag tttatttatt
     6541 ttttttgctg aatcatagtt tgtgaacaaa gaacccggat gttacataca gtacagccgc
     6601 catgttacag agagttataa cttaatcatt ttaaaaataa ttttgcctta cttttagttt
     6661 gtcatgttga gaaatgagga aatgttaaaa tgaggaaata tccaattaat ttaatatatc
     6721 aaaataatcc atgattacaa tgcactgaac tggagaaaat taagatgttt tctagtgtca
     6781 tgaaacaaat gtaagagatg tacattgtag atgttttatg tcaagaattg gctagttgat
     6841 gcagcatact ggcgatactc agttgtaata acagtaacgt tacatgttaa tagactactg
     6901 agtatgctgt tctgtctatg tatgctctgt aagctacgag aaggactttt ttaaacagta
     6961 aagggtgcaa tatttttaca aattgaatta aataaaggct gtctattaag taatatgctt
     7021 gatatttttc ttacttgatc ggaaataaga aaaaatataa acgttgttgc tctaaaaatc
     7081 ctagttcagt ttagccaacc acaaatacct ttttgttcct ccaacagttt ttttttcttc
     7141 tctataatat ttggcagtct atagtactcc aaatgtttcc ccacagtcta actaattggt
     7201 acagccaaaa tcatgacact tattgcaata ataattttgg ttcattggca ttgttgatag
     7261 cctgtgccac taatatggtc gattgatcat gcttcaggaa gaaaactata ttgtttgatg
     7321 taagattatt aaatcttcac ctgcttccat tacaaactat tcccatctta ttgaattctg
     7381 gtatgtctta aaggattagt tcacttccca aatcaaaatt tacttagcct tttttcatcc
     7441 atgatccctt tttttcatca ttaatgaaga aattgttttt gaaaaagttt caagattttt
     7501 ttctctatat tgtggagctt gttagtttaa aattccaaaa tgcaatatgt ggcttcaaat
     7561 ggttctaaat gatcccagtc aaggaataac agtcttatct aatgaaacca ttagaccttt
     7621 aaaaataaaa aaaataaaag tatttatttt taaatgactg agtgattaag ttgaatttca
     7681 gcgtttcctt actgtgtaga agtccttcct tactggcccc accctttggt tctctgccaa
     7741 tctgctacct aatgtaatgt tgtggaacat tattattctt tatttcttaa ttttttattt
     7801 tttattttaa aacaatgtaa actgcacaga tgtgcagttt gtttaaaatg gccaatgctt
     7861 tggaaatgca tgacataatt agatttcatg atgcacaaag ccaaatctca gagcttgtgc
     7921 aaaatgagct atcatttcac taggtaagac cctaaatttt catataggat catttggaca
     7981 attttgctgc aggtaaaatg cattctatag tccactgtca gccattgttt tggatagtat
     8041 ttatttttct ctacaagtat agtcaatagt tttctattat tttaaaggtt tgtaacattt
     8101 aagggtgacc aaatgcaaag taaaatttca ttttcgggtg aactatctcg tttaacatgg
     8161 gagaagtgca aaacatacat tattggctag aacattgtag tattttttaa atggaaatgt
     8221 gtgattgcta atcttacttt gaatttgttt acagggatcc tttaagggcg aattcgaccc
     8281 aagtttgtac aaaaaagcag gctccgccac catggtgccc aagaagaaga ggaaagtctc
     8341 caacctgctg actgtgcacc aaaacctgcc tgccctccct gtggatgcca cctctgatga
     8401 agtcaggaag aacctgatgg acatgttcag ggacaggcag gccttctctg aacacacctg
     8461 gaagatgctc ctgtctgtgt gcagatcctg ggctgcctgg tgcaagctga acaacaggaa
     8521 atggttccct gctgaacctg aggatgtgag ggactacctc ctgtacctgc aagccagagg
     8581 cctggctgtg aagaccatcc aacagcacct gggccagctc aacatgctgc acaggagatc
     8641 tggcctgcct cgcccttctg actccaatgc tgtgtccctg gtgatgagga gaatcagaaa
     8701 ggagaatgtg gatgctgggg agagagccaa gcaggccctg gcctttgaac gcactgactt
     8761 tgaccaagtc agatccctga tggagaactc tgacagatgc caggacatca ggaacctggc
     8821 cttcctgggc attgcctaca acaccctgct gcgcattgcc gaaattgcca gaatcagagt
     8881 gaaggacatc tcccgcaccg atggtgggag aatgctgatc cacattggca ggaccaagac
     8941 cctggtgtcc acagctggtg tggagaaggc cctgtccctg ggggttacca agctggtgga
     9001 gagatggatc tctgtgtctg gtgtggctga tgaccccaac aactacctgt tctgccgggt
     9061 cagaaagaat ggtgtggctg ccccttctgc cacctcccaa ctgtccaccc gggccctgga
     9121 agggatcttt gaggccaccc accgcctgat ctatggtgcc aaggatgact ctgggcagag
     9181 atacctggcc tggtctggcc actctgccag agtgggtgct gccagggaca tggccagggc
     9241 tggtgtgtcc atccctgaaa tcatgcaggc tggtggctgg accaatgtga acattgtgat
     9301 gaactacatc agaaacctgg actctgagac tggggccatg gtgaggctgc tcgaggatgg
     9361 ggactgacac ccagctttct tgtacaaagt tgatgtacca tcgatgatga tccagacatg
     9421 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     9481 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     9541 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     9601 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagatcgt
     9661 gcatgcctcg agaaaattgt gaaatgcaca gaatgtacat atacaataca tttcctacaa
     9721 taatgctctc ttttctatta atagtgtgca ttcagtgcag gatgttatgt atttattttc
     9781 tgtagtgcaa actttttcaa agaattttcc agtctaagct tttcagtcaa gaaaaaaaaa
     9841 agcgattgat tatctgtgat atttaaaaaa attagctctt aaatctggtg aaccaagtgg
     9901 ccacaacaca ttaaatactg acgttctaca gccatgtagc ttcatctggt ctggtttgtt
     9961 ttggcaggca cttattttcc ggtgatagtt ggccacaaga gtattgtctg agccattcag
    10021 tgctagactg tcattctc
//
