LOCUS       sequence-344885-        4789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MusF1 prenyltransferases from Nmu PCC 7906.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Mattila A, Andsten RM, Jumppanen M, Assante M, Jokela J, Wahlsten M,
            Mikula KM, Sigindere C, Kwak DH, Gugger M, Koskela H, Sivonen K, Liu
            X, Yli-Kauhaluoma J, Iwai H, Fewer DP
  TITLE     Biosynthesis of the Bis-Prenylated Alkaloids Muscoride A and B.
  JOURNAL   ACS Chem Biol. 2019 Dec 20;14(12):2683-2690. doi: 
            10.1021/acschembio.9b00620. Epub 2019 Nov 19.
  PUBMED    31674754
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.9b00620"; journalName: "ACS Chem Biol"; date: 
            "2019-12-20- 20"; volume: "14"; issue: "12"; pages: "2683-2690"
COMMENT     Sequence Label: pTVKRSF224
FEATURES             Location/Qualifiers
     source          1..4789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(180..1262)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1224..1243
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1263..1340)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1386..1405
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1386..1404
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1405..1429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1485..1502
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1509..1799
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             2728..2772
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(2810..2828)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2824..2871
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(3101..3916)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3101..3916)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3824..3843
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(3917..4008)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4024..4773)
                     /direction=LEFT
                     /label=RSF ori
                     /note="Plasmids containing the RSF 1030 origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     complement(4034..4051)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4274..4293)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa gttgtaattc
       61 tcatgttagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg
      121 gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct
      181 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      241 gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag
      301 acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc
      361 acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa
      421 catgagctgt cttcggtatc gtcgtatccc actaccgaga tgtccgcacc aacgcgcagc
      481 ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc
      541 gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca
      601 ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc
      661 cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt
      721 tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag
      781 aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta
      841 gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc
      901 ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt
      961 cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc
     1021 gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac
     1081 gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc
     1141 gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg
     1201 gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc
     1261 acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt
     1321 ttgcgccatt cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag
     1381 gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc ctgtagaaat
     1441 aattttgttt aactttaata aggagatata ccatgggcag cagccatcat catcatcatc
     1501 acagcactag tgactcagaa gtcaatcaag aagctaagcc agaggtcaag ccagaagtca
     1561 agcctgagac tcacatcaat ttaaaggtgt ccgatggatc ttcagaaatc ttctttaaga
     1621 tcaaaaagac cactccttta agaaggctga tggaagcgtt cgctaaaaga cagggtaagg
     1681 aaatggactc cttaagattc ttgtacgacg gtattagaat tcaagctgat cagacccctg
     1741 aagatttgga catggaggat aacgatatta ttgaggctca cagagaacag attggtggat
     1801 ccgtgactta taaaccgatt caaactgata gcgaacctta cttagaatac atcggtaaac
     1861 ataaacgtgc ctttgacatt tacgaagacc tttatcccgt taaacttttt gaagattttg
     1921 tgcaagtaga gggtagacaa ggaggtatgt ttcgctgcgg ttgtaatgta actaaagatg
     1981 aattgtctcc ggctcggttt gctcttttct ttcctacaca agacgatttg caattgcttc
     2041 acaaccccca acaacagctc gaagcagcct ttgacttctt tcgcaaagtg gaaggtcgag
     2101 cagaagtcaa agtcaattat catctgattg aaaagttttt tggtaagaat cctgattttc
     2161 gagggattta ttttattgct gtgggtgtag atgcacaaac agaaattgct gagtcacgct
     2221 taaagctatt tattcatttg agtaatgccc ccgacaagat agaatccgcc attgctttgt
     2281 gtggtgatag cccaacattg cgagcattct tagtcaacaa tgatttatta gtaggttttg
     2341 atttttattt agatggtcgc agtgaaattg agatttatcc aactatctat caagaagagt
     2401 tggaacgggc tgatattcaa agcaggatac taccactttt acccccgcgt gctttaccac
     2461 tattaaagca gtgtgaagtc ttgcaagtcg gctttagtcc ggcgaataaa agtaatattc
     2521 tttatttcga ttttgttagc gatcccaata cctttatcga taatcttggt aatgagatgg
     2581 caaaaaaagt tcatgcctat taccgccatc aacctgtcaa acatgtacta gttggtattt
     2641 ctgaaaaaga cttgtatgct agctcaattg agaaagtgaa gttgtattac ttcaagaagt
     2701 aagcttgcgg ccgcactcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag
     2761 cacatggact cgtctactag cgcagcttaa ttaacctagg ctgctgccac cgctgagcaa
     2821 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaacct
     2881 caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag
     2941 caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgacaag ctgacgaccg
     3001 ggtctccgca agtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3061 ctaaatacat tcaaatatgt atccgctcat gaattaattc ttagaaaaac tcatcgagca
     3121 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     3181 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     3241 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     3301 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     3361 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     3421 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     3481 cgcggtcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     3541 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     3601 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     3661 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     3721 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     3781 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     3841 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc
     3901 gttgaatatg gctcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3961 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggcatgcagc
     4021 gctcttccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg
     4081 tgtcagctca ctcaaaagcg gtaatacggt tatccacaga atcaggggat aaagccggaa
     4141 agaacatgtg agcaaaaagc aaagcaccgg aagaagccaa cgccgcaggc gtttttccat
     4201 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagccagag gtggcgaaac
     4261 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4321 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4381 ctttctcata gctcacgctg ttggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4441 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4501 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccat tggtaactga
     4561 tttagaggac tttgtcttga agttatgcac ctgttaaggc taaactgaaa gaacagattt
     4621 tggtgagtgc ggtcctccaa cccacttacc ttggttcaaa gagttggtag ctcagcgaac
     4681 cttgagaaaa ccaccgttgg tagcggtggt ttttctttat ttatgagatg atgaatcaat
     4741 cggtctatca agtcaacgaa cagctattcc gttactctag atttcagtg
//
