LOCUS       sequence-344893-        8511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR vector for generating Flnc STREAMING-tag KI cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Ohishi H, Shimada S, Uchino S, Li J, Sato Y, Shintani M, Owada H, 
            Ohkawa Y, Pertsinidis A, Yamamoto T, Kimura H, Ochiai H
  TITLE     STREAMING-tag system reveals spatiotemporal relationships between 
            transcriptional regulatory factors and transcriptional activity.
  JOURNAL   Nat Commun. 2022 Dec 20;13(1):7672. doi: 10.1038/s41467-022-35286-2.
  PUBMED    36539402
REFERENCE   2  (bases 1 to 8511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-20"; volume: "13(1):7672. doi"; pages: " 
            10.1038/s41467-022-35286-2"
COMMENT     Sequence Label: eSpCas9-EF-5Flnc
FEATURES             Location/Qualifiers
     source          1..8511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        651..899
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     651..671
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     822..841
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        1103..1388
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          1661..1888
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1900..1909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1909..1974
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1981..2001
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2026..4563,4564..4566,4567..5028,5029..5031,5032..5199,
                     5200..5202,5203..6126)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system, mutated to improve targeting 
                     specificity (Slaymaker et al., 2016)"
                     /label=eSpCas9(1.1)
                     /note="carries the mutations K848A, K1003A, and R1060A"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6127..6174
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(6202..6219)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6208..6415
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6424..6564
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6424..6553
                     /label=AAV2 ITR
     rep_origin      6639..7094
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6726..6745)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6936..6957
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7111..7130)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7230..7252
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7290..7308)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7376..7480
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7481..7549,7550..8341)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7699..7718)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt gagggcctat
      661 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
      721 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
      781 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
      841 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
      901 tccagcagaa cacattcacg gtttaagagc tatgctggaa acagcatagc aagtttaaat
      961 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga
     1021 gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca
     1081 gacaaatggc tctagaggta cccgttacat aacttacggt aaatggcccg cctggctgac
     1141 cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac
     1201 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     1261 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc
     1321 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     1381 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
     1441 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
     1501 gggggggggg ggaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg
     1561 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
     1621 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct
     1681 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
     1741 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagctgag
     1801 caagaggtaa gggtttaagg gatggttggt tggtggggta ttaatgttta attacctgga
     1861 gcacctgcct gaaatcactt tttttcaggt tggaccggtg ccaccatgga ctataaggac
     1921 cacgacggag actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc
     1981 ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc
     2041 atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
     2101 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
     2161 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
     2221 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
     2281 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
     2341 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
     2401 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
     2461 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
     2521 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     2581 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     2641 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
     2701 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
     2761 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     2821 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     2881 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     2941 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     3001 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     3061 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     3121 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     3181 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     3241 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     3301 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     3361 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     3421 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     3481 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     3541 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     3601 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     3661 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     3721 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     3781 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     3841 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     3901 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     3961 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     4021 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     4081 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     4141 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     4201 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     4261 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     4321 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     4381 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     4441 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     4501 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
     4561 ctggccgacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     4621 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     4681 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     4741 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     4801 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     4861 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     4921 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     4981 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctgc gctggaaagc
     5041 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     5101 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     5161 aagaccgaga ttaccctggc caacggcgag atccggaagg cgcctctgat cgagacaaac
     5221 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     5281 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     5341 aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     5401 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
     5461 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     5521 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     5581 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     5641 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg
     5701 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     5761 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     5821 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     5881 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     5941 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac
     6001 tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc
     6061 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     6121 ggcgacaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagtaagaa
     6181 ttcctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     6241 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     6301 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     6361 tggggcagga cagcaagggg gaggattggg aagagaatag caggcatgct ggggagcggc
     6421 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     6481 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     6541 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     6601 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     6661 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
     6721 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6781 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6841 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6901 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6961 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
     7021 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     7081 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     7141 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     7201 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     7261 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     7321 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     7381 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7441 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7501 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7561 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7621 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7681 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7741 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7801 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7861 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7921 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7981 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     8041 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8101 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8161 gtttattgct gataaatctg gagccggtga gcgtggaagc cgcggtatca ttgcagcact
     8221 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8281 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8341 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8401 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8461 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
