LOCUS       sequence-344897-         351 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Eleventh beta-strand of GFP (GFP11) fused to a mitochondria signal 
            sequence for mitochondrial targeting, HA tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Cortes Sanchon A, Santhosh Kumar H, Mantovani M, Osinnii I, Mateos 
            JM, Kaech A, Shcherbakov D, Akbergenov R, Bottger EC
  TITLE     ER-misfolded proteins become sequestered with mitochondria and 
            impair mitochondrial function.
  JOURNAL   Commun Biol. 2021 Dec 2;4(1):1350. doi: 10.1038/s42003-021-02873-w.
  PUBMED    34857875
REFERENCE   2  (bases 1 to 351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-12-2"; volume: "4(1):1350. doi"; pages: " 
            10.1038/s42003-021-02873-w"
COMMENT     Sequence Label: pMTS-HA-GFP11
FEATURES             Location/Qualifiers
     source          1..351
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             235..261
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             301..348
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 atggcctcca ctcgtgtcct cgcctctcgc ctggcctccc ggatggctgc ttccgccaag
       61 gttgcccgcc ctgctgtccg cgttgctcag gtcagcaagc gcaccatcca gactggctcc
      121 cccctccaga ccctcaagcg cacccagatg acctccatcg tcaacgccac cacccgccag
      181 gctttccaga agcgcgccta ctcttccgga tccggtgacg gtccggtcgc cacctaccca
      241 tacgatgttc cagattacgc tggtcgacgg atcggtggtg gttctggagg cggttctatg
      301 agagatcata tggttttgca tgaatatgtt aatgctgctg gtattactta a
//
