LOCUS       sequence-34490-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  TITLE     Barton Lab Unpublished Plasmids
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Flag-TRIM24-F869D/F979A/N980A shRescue
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      68..77
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             80..103
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             107..130
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcc accatggcag actacaagga cgacgatgac aaggcagact acaaggacga
      121 cgatgacaag ggtacctcta gaagatctag gacaatggag gtggcggtgg agaaggcggt
      181 ggcggcggcg gcagcggcct cggctgcggc ctccgggggg ccctcggcgg cgccgagcgg
      241 ggagaacgag gccgagagtc ggcagggccc ggactcggag cgcggcggcg aggcggcccg
      301 gctcaacctg ttggacactt gcgccgtgtg ccaccagaac atccagagcc gggcgcccaa
      361 gctgctgccc tgcctgcact ctttctgcca gcgctgcctg cccgcgcccc agcgctacct
      421 catgctgccc gcgcccatgc tgggctcggc cgagaccccg ccacccgtcc ctgcccccgg
      481 ctcgccggtc agcggctcgt cgccgttcgc cacccaagtt ggagtcattc gttgcccagt
      541 ttgcagccaa gaatgtgcag agagacacat catagataac ttttttgtga aggacactac
      601 tgaggttccc agcagtacag tagaaaagtc aaatcaggta tgtacaagct gtgaggacaa
      661 cgcagaagcc aatgggtttt gtgtagagtg tgttgaatgg ctctgcaaga cgtgtatcag
      721 agctcatcag agggtaaagt tcacaaaaga ccacactgtc agacagaaag aggaagtatc
      781 tccagaggca gttggtgtca ccagccagcg accagtgttt tgtccttttc ataaaaagga
      841 gcagctgaa
//
