LOCUS       sequence-344911-        5216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses myc-tagged version of wild-type protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Marchak A, Grant PA, Neilson KM, Datta Majumdar H, Yaklichkin S, 
            Johnson D, Moody SA
  TITLE     Wbp2nl has a developmental role in establishing neural and 
            non-neural ectodermal fates.
  JOURNAL   Dev Biol. 2017 Sep 1;429(1):213-224. doi: 
            10.1016/j.ydbio.2017.06.025. Epub 2017 Jun 27.
  PUBMED    28663133
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2017.06.025"; journalName: "Dev Biol"; date: 
            "2017-09-1- 1"; volume: "429"; issue: "1"; pages: "213-224"
COMMENT     Sequence Label: pCS2+5'MT-wbp2nl
FEATURES             Location/Qualifiers
     source          1..5216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             103..132
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             142..171
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             181..210
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             220..249
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             259..288
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             322..351
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1268..1402
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1292..1311)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1346..1365
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1515..1535)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1515..1533)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1554..1570)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1554..1570)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1567..1589)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1578..1594
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1602..1608,1609..1626,1627..1632))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1647..1668
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1785..1802)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1956..2544)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2036..2055)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2715..3506,3507..3575))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3338..3357
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3576..3680)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3706..4161)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3843..3864)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4055..4074
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga tttaaagcta tggagcaaaa gctcatttct
      121 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg
      181 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa
      241 gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag
      301 agcttgggcg acctcaccat ggagcaaaag ctcatttctg aagaggactt gaattcgtca
      361 ctcaataagg accactctca gggggccggg atcattgtca gcaacgggga gagtattctc
      421 agacagtgta aagatgtgga actgtccttc agcgatatgt ctcacaaatc agaagcattc
      481 agaggcacaa agaaaggatc gctatttctc accgcttaca gggtgatctt cctaaacaag
      541 gggaaggatc ccatgatgtc ctttaatatg ccattctaca tgatgaaagg atgctccata
      601 gaacagccag tgttctctgc caactacatc aagggaacca tcagtgctga acctggaggt
      661 ggctgggagg gtcaagcctc ttttaaaatt acatttaata gtggaggtgc tattgaattt
      721 ggacaaatca tgttcaaaat ggcaacttgt gcttccagaa cccctcctgt tcctcatgct
      781 gcatatggat acacacctgc tccaggaggc tatgcacatg gcgggtaccc tccagctcca
      841 ggaatatata cacccccacc ccaaccatca ggcccttatc catatggacc ccctgctatg
      901 aatggatatg gaccacctcc aaatcctatg ggatacccgt atgctccacc agcaggacct
      961 ggcatgtatc caccccctcc tgaaatgaat cccatttaca tggctcctcc accaccttat
     1021 cctggacccc cctacaatgg gaccccctac aatggaaccc cagcaccttc tgctcccact
     1081 ggatgcatga atgctggtat gcctggaggt agcaaagctg cagaagctgc atccagtgcg
     1141 tattataacc cagctgaccc tcacaatgtc tatatgccta tggatcgtcc tcctccatac
     1201 gctccaaatg atgataaaaa gaacaattaa ctcgagcctc tagaactata gtgagtcgta
     1261 ttacgtagat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     1321 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     1381 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     1441 gggggaggtg tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggcccggta
     1501 cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     1561 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccgggagc
     1621 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     1681 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     1741 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     1801 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1861 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1921 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     1981 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2041 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2101 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2161 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2221 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2281 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2341 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     2401 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2461 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2521 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2581 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     2641 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     2701 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     2761 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     2821 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     2881 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     2941 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3001 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3061 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3121 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3181 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     3241 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3301 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     3361 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     3421 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     3481 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     3541 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     3601 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     3661 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     3721 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     3781 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     3841 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     3901 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     3961 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     4021 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     4081 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     4141 atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc
     4201 gatcggtgcg ggcctcttcg ctattacgcc agtcgaccgc caattcaata tggcgtatat
     4261 ggactcatgc caattcaata tggtggatct ggacctgtgc caattcaata tggcgtatat
     4321 ggactcgtgc caattcaata tggtggatct ggaccccagc caattcaata tggcggactt
     4381 ggcaccatgc caattcaata tggcggactt ggcacagggc caagacccct cctcaaactt
     4441 ggcaccgtgc caagtagacc cctccccagt tggcacagtg ccaggtccgc catattgaat
     4501 tggcatggtg ccaataatgg cggccatatt ggctatatgc caggatcaat atataggcaa
     4561 tatccaatat ggccctatgc caatatggct attggccagg ttcaatacta tgtattggcc
     4621 ctatgccata tagtattcca tatatgggtt ttcctattga cgtagatagc ccctcccaat
     4681 gggcggtccc atataccata tatggggctt cctaataccg cccatagcca ctcccccatt
     4741 gacgtcaatg gtctctatat atggtctttc ctattgacgt catatgggcg gtcctattga
     4801 cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa tggcccgcct ggctcaatgc
     4861 ccattgacgt caataggacc acccaccatt gacgtcaatg ggatggctca ttgcccattc
     4921 atatccgttc tcacgccccc tattgacgtc aatgacggta aatggcccac ttggcagtac
     4981 atcaatatct attaatagta acttggcaag tacattacta ttggaagtac gccagggtac
     5041 attggcagta ctcccattga cgtcaatggc ggtaaatggc ccgcgatggc tgccaagtac
     5101 atccccattg acgtcaatgg ggaggggcaa tgacgcaaat gggcgttcca ttgacgtaaa
     5161 tgggcggtag gcgtgcctaa tgggaggtct atataagcaa tgctcgttta gggaac
//
