LOCUS       sequence-344918-       13687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the DisCas7-11 full locus along with a DR and golden gate 
            site for cloning spacers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 605 to 639)
  AUTHORS   Ozcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, 
            Koonin EV, Abudayyeh OO, Gootenberg JS
  TITLE     Programmable RNA targeting with the single-protein CRISPR effector 
            Cas7-11.
  JOURNAL   Nature. 2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 
            10.1038/s41586-021-03886-5.
  PUBMED    34489594
REFERENCE   2  (bases 1 to 13687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 
            10.1038/s41586-021-03886-5."
COMMENT     Sequence Label: pDF0250 DisCas7-11 full locus with Golden gate...
FEATURES             Location/Qualifiers
     source          1..13687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(23..568)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(103..122)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        605..639
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     CDS             complement(12422..13081)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     12996..13015
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(13082..13184)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(13576..13593)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa
       61 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      121 cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
      181 cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt
      241 cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc
      301 ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc
      361 actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga
      421 aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt
      481 agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga
      541 gattacgcgc agaccaaaac gatctcaaga agatcatctt attaatcaga taaaatattt
      601 ctagttgatg gctagctcag tcctaggtac agtgctagcg gttggaaagc cggttttctt
      661 tgatgtcacg gaaccgagac ctactgttgg tctcgggttg gaaagccggt tttctttgat
      721 gtcacggttg ccacccggat cccatgtgcg ctgtaccggt ggttctccga ctgatcaaag
      781 accgggcagc cccggatgaa ttttacatcc ggggctgttc cgcaggcaaa atgccagaga
      841 attctgagca ccggaatctg acgacataac aaaaggcaaa gttgactgaa agaacaggaa
      901 cattatctga aatttttaca aaaaggaggc agatatggtg acacccggca tgattcagag
      961 cgcaggcaaa gagatgggac ttttcatccc gtgggatgtg gcattgggca tggttctctg
     1021 cgttgccgga aaaatactca tgggcgagag cctggaaaat gcggttttgt cgtgttggaa
     1081 ataaagccca ttttaaaatg tcgcggataa gatgaataat accaaatcgc agatggaata
     1141 tgtcagccgg agcaaggctc cggcaatccg cactccgaac aaaagataaa ttcaatacag
     1201 aatcggtaga agcagggatt ttttttattg acgctgataa caggaaaaag ctacaaacgt
     1261 agtttgcaga tttttatcat tacctacaac tttttttaaa atcaggatat tcgaaaatta
     1321 tgagtttaaa tcatccctgt ttgtctgccc gtgctgcgtc acccgaatgt actgtttgtc
     1381 ctgtttttaa ggctgggcat caggaggcat ttgtcaaaat tcttgaaaaa aaggttcggt
     1441 ttctcgaaag ccgaaaacat ctgtcaccgg aagacaagga agatattctc agcgaatttg
     1501 ttctgcaaat ccagtcttcc acatcctcat ttaagggaca gaatgcaaaa tttgaaacct
     1561 gggcctatag aatatttcaa aatgtatggg ctgatttttt tagaaaaatc aaaaatttca
     1621 aaacgcagag aaccgatgac ggcatttata ttcaggaaac aataaaagta aaaaaaacgg
     1681 ataaagacgg aagagaaatg cttgaggaaa aagcaaggac gggcgtattt cttccatata
     1741 aagagaatat cgaagacaga attgaatcgc gggaaatttt ttcaattttc cgcaaagcag
     1801 taaaagatcc gaaaaaggca ttttgctatc atttgcttgt taatcattat acttggaaaa
     1861 gcgaagggtt gaaacaggat gaaatggcgg ctcggtatga aatgaggcac gataccttca
     1921 gaaaacgcct ttcagaatgc cgctcaagat tcaaacaata tctgaaagag gttacgcaat
     1981 gacaattccc gaaatctgcc gcgagccagg atatgttttt tacgaaggaa tttttgaaag
     2041 tgttctttcg cgtgactgtt attgctttaa agtggagaac gaggattaca gcggagaggt
     2101 gataatggaa aaacattatt cccccgacaa tgtgcagcta aaaagcggta aacctgtcac
     2161 tgaccttgtt gttgttagat atgagaaaaa ccgtctgttt gtggccatga ataattattt
     2221 tgatgatctg atggggcatt ttttcaatac ggacagagga gtttttgtaa aaaatattcc
     2281 ttttccaaac tgggaaaggg cttgggaaac agcgtttgcc catacaaatt atcagcctga
     2341 aaagttgcaa ctcaagaaga gagaacacag aaacgaacga tggattgagg taaaaagtcc
     2401 ccggtgtcgg atacctgccc atgaacattc tcatatcagt gatatggacg gggaaccagt
     2461 gttttttcct gcctcatttt acaatataca aaacagagac aggaacggca ggttgattcc
     2521 ccgcgcactg tataaaaccg aaggcatgga ggagatgccc tgttacttca aaggggaatt
     2581 cagcccggaa tccggcaaca cccggacagt ccacatttat ctgcatggca taacgtttcc
     2641 ttccaaatgg cattatagcg tattgaagca catgatctgt cttccgaaat tcaaaggcaa
     2701 ggatgacaat cgcgacctgt ggaaagcatt tgatggagaa gaaccgcttg aaccagacgt
     2761 agaaattgtg caagcgtgta aactgaaatg caaagattca tgtgattaaa ggcattgcca
     2821 aaaggaaaaa ttttcaagaa tgttcacaaa aagatcagaa cagcgtgtga acggtatctg
     2881 agacgggcca tcatattttt tccccaacat accgacaaag ctctgaggat ttggcaagac
     2941 aggaagagcc agtgcggaaa acccaaattt attgaggtgg attgtctgaa taaaatcgaa
     3001 aacaacatat tcagacaagc tctcgggcag gaagagttgg agcggaatga aattctcatc
     3061 ctccggggat attcatatga ctgggattcc gccgaggaac caacgattca tctgagtatt
     3121 gtcacaatgt atgcaggccc cttaaaaggg aaaatgcccc gtgaaaacgc attcaaaaaa
     3181 gatttcaggc tgaccggaaa cggcaaactt tccatatcgg agaattaaga gacaaaatga
     3241 atacaacaac atacaacacc acaacagacg cgcttttgga atggggcaag gtatattttc
     3301 agaaagagga tttttctgaa ttccttgata atctcgaagc gtatatttca gatgccgggg
     3361 acagtctgaa agatgaactt gagagcgggg tggaaaagct tgttttgggc ataaaatcgg
     3421 cggaagcagt tattttcgga gaggctgtga tcgggactac gccggaaaat gaagcatggt
     3481 acgatgccga agaatccttt ctgactttgg attgtgccgt atggctgtcc caagcgcttg
     3541 atcgcgttgt tcgcaggcag gatgcgtcat tggccgacag cctgatcgcc cgcttggacg
     3601 aagccataaa ccgcgtcgcc gaaaaactgt atgcggacaa tttatctccg cttcgttttt
     3661 catcgctcaa tgaaatccgt cgaagcgctc tggaggcaac ggatgagaaa tatcattatc
     3721 tgtttccctg gcacggggcc gcatgtgatg ttgatgaaaa cattctgctg attctgacgg
     3781 aggaatatca tctgatcggg gcagataaag ccagagccaa tctttcagag gaactgcggg
     3841 gcgacctgcc ttttattttt gccgagttgg agcgggatga ggtgctgcgg gcatatgttg
     3901 aaaaggagaa cgccctttct cttgctttgg aaaataccat gcgggaacac tgggcattcg
     3961 gcctgctgga agcggcccgt gatgaagggt acaaccatcc ctatcccgca gatgtcggga
     4021 tgaggattca tcaggttgcc cgtgccgtat tttcccagac aaacctctct ccggcggaac
     4081 gccttgctgt tgccatagcc ggggcctgtt ttacgcctga gatcagcgaa gaccggcggc
     4141 ttgagattct gctggactgt gaggagcggg tgtgtgaaat cgaagccccg accggagacg
     4201 atacatccgt ccgcgttatt aaagacttga aagcgctggc caatcaccgt gtccgccatg
     4261 aaatcccggc agaaagtctt gtcagtctgt ggtttgagca gattgaggcg gcggggacgg
     4321 attttgatac aaagacaccg atggatgaat tggtgttgcg aatgctttcc gataacgtca
     4381 tcactctgtc tgttgaccga aaagcggctt ctcagacaga aacagatgat gtgaaaccgc
     4441 agaaaggaaa aatcataccc tttcctgttc ctgatattgc caacgatgaa gttgaatacc
     4501 aaaaagctgt gggaatgaag aaggataaaa aggcgactaa tgacagcaaa gtcaaatttc
     4561 ccggcctgct tgaaatccag ggttgtcgtg acggggacaa ggctattttg ctggaagaca
     4621 cagacgatgc ggcagctaat caccggaaac tgttttccat tctgaaagca ggtaaattga
     4681 attcagcttt tttcattcaa agcgatgatg gagaatgggt tgagtcggaa tccaaaccga
     4741 cgatggaaga taaccgtatc atattacatg acagccatca ttccagcttt gtatggatat
     4801 tggataccgg ttccatgcag ctcagacaga gtgtaaagtg tgtcaaagac gcattgaata
     4861 agaagacagg gagtgcgaag aaactgaaac cgaaaacaat gatcgtttgg gtcacgattc
     4921 cgcaggaggg atgatatgtc aaatccgatt cgggatattc aggacaggct gaaaactgca
     4981 aaatttgaca ataaggatga tatgatgaac ttggcatcct cgttgtacaa atatgaaaag
     5041 caactgatgg attcttctga agcaacactt tgtcagcaag ggttgtccaa tcggcccaat
     5101 tcattcagcc agctttccca attccgggat tccgacattc aaagtaaagc aggcggacag
     5161 acggggaaat tttggcagaa tgaatatgaa gcctgcaaaa attttcaaac ccataaagag
     5221 cggcgggaaa ctctggagca aatcatccgc tttctccaaa atggcgcaga agaaaaagat
     5281 gcggatgatc tgcttctgaa aacgcttgcc cgcgcctatt ttcacagggg gttgctctac
     5341 aggcccaaag gcttctctgt tccggccagg aaagtcgaag caatgaaaaa ggcaatagcg
     5401 tactgtgaaa taattctgga caaaaatgaa gaagagtcgg aggcactaag aatctggctt
     5461 tatgccgcaa tggaactccg acggtgcggt gaggagtatc cggaaaattt cgctgaaaaa
     5521 cttttttatc tcgcaaatga cggtttcata tccgaactgt atgatatcag actttttctt
     5581 gaatatacgg agcgagaaga agataacaat tttcttgata tgatccttca ggaaaatcag
     5641 gacagggaac gcctttttga actatgtctt tacaaggcca gagcgtgttt ccatctaaat
     5701 cagttgaatg acgtgagaat atatggagaa agtgcaattg ataacgcgcc gggggctttt
     5761 gccgatccgt tctgggatga gcttgttgaa tttatcagaa tgcttagaaa taagaaatcg
     5821 gaattatgga aagaaattgc cataaaagcg tgggacaagt gccgcgaaaa agaaatgaaa
     5881 gttggcaaca atatttatct aagctggtat tgggccagac agcgagagct ttatgacctt
     5941 gcgtttatgg cgcaggacgg tattgaaaaa aaaacgagaa ttgctgattc cctgaaatcc
     6001 agaaccacac tcagaatcca ggaattgaat gaactcagaa aagatgcaca tagaaaacaa
     6061 aataggagat tagaagacaa actggatagg attattgagc aggaaaatga agccagagat
     6121 ggtgcttatc taaggagaaa tcctccttgc tttacaggcg gaaaaaggga agaaatacct
     6181 tttgccagac tgccccaaaa ctggattgcc gtccatttct atctgaacga actggaatct
     6241 cacgaaggcg gaaaaggcgg acatgcgctg atttatgatc ctcaaaaagc tgaaaaagat
     6301 caatggcagg acaaatcctt tgactacaaa gaactgcatc ggaaattttt ggagtggcag
     6361 gaaaactata ttctgaacga agaagggtcc gccgattttc tggtaacact ctgtcgggaa
     6421 atcgaaaaag ccatgccttt tctgtttaag tcagaagtga ttcccgaaga caggccggta
     6481 ttgtggattc cgcatggttt cctgcatcga ctcccccttc atgcagccat gaaatcaggt
     6541 aataactcca atattgaaat attttgggaa agacatgcgt ctcgatatct tcctgcatgg
     6601 catctgtttg atcctgctcc atatagtaga gaagaaagct ccactctttt aaagaatttt
     6661 gaagaatatg atttccagaa tttggaaaat ggagaaattg aggtttacgc cccctcatct
     6721 cccaagaaag taaaagaggc aattagggaa aatccggcca ttctgctttt gctttgtcat
     6781 ggtgaagccg acatgaccaa tccttttcgc tcctgcctga agcttaaaaa taaagatatg
     6841 accatctttg atctgctgac agtggaagat gtaaggctct ccggttcacg aattcttctg
     6901 ggtgcctgtg agtccgatat ggttccaccg ttggagtttt ctgtggacga acacctttct
     6961 gttagcggtg catttctttc tcacaaggcg ggagagattg ttgcgggtct atggacagtt
     7021 gattcggaaa aagttgacga atgctattcg tatttggtgg aggagaaaga ttttttgaga
     7081 aatttgcagg aatggcaaat ggcagaaaca gaaaactttc ggtctgaaaa tgatagttct
     7141 ttattttata aaatcgcccc gttcagaatc atcggcttcc cggcggaatg atataaccca
     7201 cacgtcttcc ttctttcccg atccagcaga gctacaggga aagcgtataa gaacgggaaa
     7261 aattcaggcc ggagcgggac tttccttttc tcacacattt tccctgtagt tttcccaagg
     7321 ctttgaggga cgatttccac gcgacatacc gaggagacac ataccatgac gacgactatg
     7381 aaaatttcca tcgaatttct cgaacccttt cggatgacta aatggcagga aagtaccaga
     7441 cgaaacaaaa acaacaaaga attcgtccgg ggtcaggcat ttgccagatg gcacagaaac
     7501 aaaaaagaca acacaaaggg ccgtccctat atcaccggaa ccctgctccg ttcggcagtc
     7561 atccgctcgg cggagaacct gctgacgctt tccgatggaa aaatttcaga aaagacctgt
     7621 tgtccgggga aattcgacac tgaagacaaa gacagattgt tacaattgcg ccagcgttcc
     7681 acgctcaggt ggacagataa aaatccctgc ccggacaacg ccgaaacata ttgcccgttc
     7741 tgtgaactgc tgggccgatc cggtaacgac ggaaagaaag ccgaaaaaaa agattggaga
     7801 tttcgtatcc atttcggcaa tctcagtttg cctggaaaac cggactttga cggcccgaag
     7861 gcaatcggat cacagcgcgt tctgaaccgg gtcgatttca aaagcggcaa ggcccatgac
     7921 ttttttaaag cctatgaagt ggatcatacc cgctttcccc ggtttgaggg agagatcacc
     7981 attgacaaca aggtgagtgc cgaggcccga aagcttctgt gcgacagcct gaaattcaca
     8041 gaccggctct gcggggcctt gtgtgtcatc cggtttgacg agtatactcc ggctgccgat
     8101 tccggcaaac agacggagaa tgtgcaggca gagccaaatg ccaatctcgc cgagaaaacc
     8161 gccgagcaga tcatcagcat cttggatgac aacaaaaaaa ccgaatacac ccgccttctg
     8221 gctgatgcca tccgttcgct ccgccggagc agcaagcttg tcgccggtct gcccaaagat
     8281 cacgatggaa aggatgatca ttatctctgg gacatcggaa agaaaaaaaa agatgaaaac
     8341 agcgtaacaa tccgccaaat cctgacaaca tcggcggata caaaagaact taaaaatgcc
     8401 ggaaagtggc gcgaattctg cgagaagctg ggcgaggcac tctacctgaa atccaaagac
     8461 atgagcggcg gcttgaaaat aacccggcga attctgggag acgccgaatt tcacggaaag
     8521 ccggatcgtc tggaaaaatc ccgttctgtt tccatcggct ctgtgctgaa agagacggtt
     8581 gtctgcggag aactggtcgc aaaaacgccg tttttcttcg gagccattga tgaggatgca
     8641 aaacagaccg atcttcaggt gttgctgacg cccgacaaca aataccgcct gccgcgctcc
     8701 gctgttcggg gaatcctgcg ccgggatttg cagacatact ttgacagccc gtgcaatgcc
     8761 gaactcggcg gaaggccctg tatgtgcaaa acctgccgga tcatgcgcgg tatcaccgtc
     8821 atggatgccc gcagtgaata caatgcgccg ccggaaatcc gccaccgcac ccgcatcaat
     8881 ccctttaccg gcacggttgc cgagggagcg ctgttcaata tggaagtggc accggaggga
     8941 attgtgtttc ccttccagct tcgctaccgg ggaagcgaag acggattgcc ggatgcactg
     9001 aaaaccgtcc tgaaatggtg ggcagagggc caggcgttta tgagcggcgc ggcatccacg
     9061 gggaaagggc gattccgtat ggaaaacgcc aagtatgaga cattggattt gtccgatgaa
     9121 aatcaacgga atgattatct gaaaaattgg ggatggcggg atgaaaaagg attggaggaa
     9181 ctgaaaaaac gcctgaattc tggcttgccg gaaccgggca attaccgtga ccccaagtgg
     9241 cacgaaatca acgtcagtat agaaatggcc tcccccttca tcaatggcga cccgatccgc
     9301 gccgccgtgg ataaaagggg tacggatgtg gtcacgttcg tcaaatacaa agccgaaggt
     9361 gaagaggcca aaccagtttg tgcctacaag gcggagagtt tcaggggcgt gatacgttcg
     9421 gcagttgcgc gaatccatat ggaagatggt gttccgctga ccgaactgac ccacagcgat
     9481 tgcgaatgcc tgctctgcca gattttcggc agcgaatatg aggccgggaa aatccggttt
     9541 gaggatctgg tgtttgaatc agatccagag ccggtgacat tcgaccatgt ggccattgac
     9601 cggtttaccg gcggcgcggc tgacaaaaag aaatttgacg attctccgct tccgggcagt
     9661 ccggccagac ctctcatgct aaaaggcagc ttctggatac ggcgggatgt tctggaagat
     9721 gaggaatatt gcaaagcgct gggaaaggcg ctggcagatg tgaacaacgg gctttatcct
     9781 ctgggcggaa aaagcgccat cggatatggg caggtaaaaa gtctggggat taaaggagat
     9841 gataagcgga tttcccgttt gatgaacccc gcttttgatg aaacagatgt ggctgtgccg
     9901 gaaaagccga aaaccgatgc cgaggttcgg attgaggcgg aaaaggttta ttatcctcat
     9961 tattttgtcg agcctcataa aaaggtggag cgtgaagaaa agccttgcgg ccatcagaaa
    10021 tttcatgaag gacggctgac cggaaaaatt cgatgcaaac tgatcacaaa gacgccgctc
    10081 attgtaccgg acacctcgaa tgacgatttt ttcaggcccg cagacaaaga agccagaaaa
    10141 gaaaaagatg agtatcacaa aagctatgcc tttttcaggc ttcacaagca aattatgatt
    10201 cccggttccg agctgcgggg catggtcagt tcggtttacg aaaccgtgac caactcctgc
    10261 tttcgcattt ttgatgaaac caagcggctt tcgtggcgga tggatgcaga ccatcagaat
    10321 gtgcttcaag attttcttcc cggcagagtt acggctgacg gaaagcatat acaaaaattt
    10381 tctgaaaccg cacgggttcc tttttatgat aaaacccaga agcattttga catattggat
    10441 gaacaggaaa tagcaggcga aaaacctgtt agaatgtggg tcaaaagatt tatcaaacgt
    10501 ttgtctctgg tcgatcctgc taaacatcct caaaaaaagc aggacaacaa atggaagcgc
    10561 agaaaagagg gaatagccac attcatagaa cagaaaaacg gcagttatta tttcaatgta
    10621 gttacgaaca atggctgtac gagctttcat ttatggcaca aacctgacaa ctttgaccaa
    10681 gagaaacttg aaggtatcca gaatggtgag aaattggatt gttgggtgag agattccaga
    10741 tatcaaaagg ctttccagga aatacccgaa aatgatccgg atggatggga atgtaaagaa
    10801 ggataccttc atgttgtcgg tcccagcaaa gtagagtttt ccgacaagaa aggtgatgtc
    10861 ataaacaatt ttcagggaac cctgccatct gtaccgaatg attggaaaac gatccgaacc
    10921 aatgatttca aaaaccgaaa acgcaaaaat gaacccgtat tttgctgtga ggatgataag
    10981 ggaaactatt atacgatggc aaaatattgt gaaacctttt tctttgacct caaagaaaat
    11041 gaggagtatg aaattccaga aaaagcccgt attaaataca aagagctgtt gagagtttat
    11101 aacaataatc cccaagcagt tcctgaatcg gtatttcaat ctcgcgtcgc aagagaaaat
    11161 gtcgaaaagc ttaaatctgg cgatcttgtc tattttaaac acaatgaaaa atatgttgag
    11221 gatattgttc ccgtccgcat ttcccggaca gttgatgatc ggatgatcgg caaacgcatg
    11281 tcagccgatc tccgcccctg tcacggtgac tgggtggaag acggcgacct ttccgccctg
    11341 aacgcctacc cggaaaagcg ccttctcctg cgtcatccca aaggattgtg cccggcctgc
    11401 cgccttttcg gaaccggttc atacaagggc cgggttcggt tcggatttgc aagccttgaa
    11461 aatgatcccg aatggctgat acccggcaag aatcccggtg atcccttcca cgggggaccg
    11521 gtaatgctgt cgcttctgga gcgcccccgg cccacatggt cgatccccgg atcggacaat
    11581 aagttcaaag tgccgggccg gaagttttat gtccatcatc atgcatggaa aacgataaaa
    11641 gacggcaacc atcccacaac cggaaaggcc atagaacaaa gccccaacaa ccggacagtt
    11701 gaagccttgg ccgggggaaa cagcttttct tttgaaatcg cttttgaaaa tctgaaagaa
    11761 tgggaactcg gtttgctgat tcattccctg caattggaaa aagggttggc acacaagctg
    11821 ggcatggcaa aatcaatggg attcggcagc gttgagattg atgttgaatc tgtgcgtctg
    11881 aggaaggact ggaaacagtg gagaaacgga aacagtgaaa taccaaactg gcttgggaaa
    11941 ggctttgcca agctaaagga atggttcaga gatgagctgg attttataga aaatctgaag
    12001 aagttgctct ggtttccaga aggcgaccaa gcgccgagag tctgttatcc gatgctgaga
    12061 aaaaaggatg acccgaacgg aaactccggc tacgaagaac tgaaagacgg cgaatttaaa
    12121 aaggaagacc gccagaaaaa gctcaccacg ccttggaccc cttgggcctg atcttctgtg
    12181 ccgcctgata ctctcccttc gtaagatgat tcagcatgaa aagttcatta tttcagcccg
    12241 gaagttcatt ttcgggctga aacctgttgg tttcctccga taacccgctt cagcgggttt
    12301 caataactga gccggaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat
    12361 tatcacttat tcaggcgtag caaccaggcg tttaagggca ccaataactg ccttaaaaaa
    12421 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
    12481 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
    12541 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
    12601 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgacacg aaaaacatat
    12661 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
    12721 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
    12781 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
    12841 caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt
    12901 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
    12961 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
    13021 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
    13081 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
    13141 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtgt cattttcgcc
    13201 aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga
    13261 agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact
    13321 tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc
    13381 tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat
    13441 cagcgctagc ggagtgtata ctggcttact atgttggcac tgatgagggt gtcagtgaag
    13501 tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag
    13561 gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc
    13621 ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag
    13681 ggaagtg
//
