LOCUS       sequence-344921-       10889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the DisCas7-11 protein only for bacterial recombinant 
            protein expression for purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10889)
  AUTHORS   Ozcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, 
            Koonin EV, Abudayyeh OO, Gootenberg JS
  TITLE     Programmable RNA targeting with the single-protein CRISPR effector 
            Cas7-11.
  JOURNAL   Nature. 2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 
            10.1038/s41586-021-03886-5.
  PUBMED    34489594
REFERENCE   2  (bases 1 to 10889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 
            10.1038/s41586-021-03886-5."
COMMENT     Sequence Label: pDF0118 DisCas7-11 bacterial expression with
            T...
FEATURES             Location/Qualifiers
     source          1..10889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      77..665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     566..585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     819..836
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    851..990
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(916..935)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1076..1098
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1092..1283)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2595..3677)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3639..3658
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3678..3755)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3961..3980
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4064..4083
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4064..4082
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4083..4107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4122..4144
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4163..4180
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4190..4207
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(4220..4243,4244..4279,4280..4303)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             4304..4597
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     primer_bind     complement(9453..9471)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9467..9514
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(9689..9694,9695..9711,9712..9717))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(9744..9762)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9830..9934
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9935..10003,10004..10795)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10153..10172)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      781 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      841 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      901 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
      961 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     1021 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     1081 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa
     1141 agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc
     1201 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg
     1261 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg
     1321 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat
     1381 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag
     1441 agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag
     1501 ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc
     1561 cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc
     1621 gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc
     1681 taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg
     1741 cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg
     1801 gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag
     1861 aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca
     1921 ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt
     1981 atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa
     2041 tcgccgtgac gatcagcggt ccaatgatcg aagttaggct ggtaagagcc gcgagcgatc
     2101 cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg
     2161 gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg
     2221 tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct
     2281 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca
     2341 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct
     2401 cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga
     2461 cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt
     2521 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat
     2581 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     2641 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt
     2701 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt
     2761 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt
     2821 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc
     2881 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg
     2941 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga
     3001 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga
     3061 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc
     3121 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta
     3181 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat
     3241 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga
     3301 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag
     3361 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg
     3421 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca
     3481 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa
     3541 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc
     3601 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat
     3661 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc
     3721 ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg
     3781 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
     3841 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
     3901 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
     3961 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
     4021 gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata
     4081 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
     4141 gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg
     4201 cggcagccat atggctagct ggagccatcc gcagtttgaa aaaggtggtg gtagcggtgg
     4261 tggttcaggt ggtagtgcat ggtcacaccc tcagtttgag aaaatgtcgg actcagaagt
     4321 caatcaagaa gctaagccag aggtcaagcc agaagtcaag cctgagactc acatcaattt
     4381 aaaggtgtcc gatggatctt cagagatctt cttcaagatc aaaaagacca ctcctttaag
     4441 aaggctgatg gaagcgttcg ctaaaagaca gggtaaggaa atggactcct taagattctt
     4501 gtacgacggt attagaatcc aagctgatca gacccctgaa gatttggaca tggaggataa
     4561 cgatattatt gaggctcaca gagaacagat tggtggatcc atgaccacta ccatgaagat
     4621 cagcatcgag ttcttggagc ccttccgcat gacgaagtgg caggagtcca cgcgccgcaa
     4681 taagaacaat aaagagtttg tgcgtggcca agcgtttgcc cgttggcacc gcaacaagaa
     4741 agataacacc aagggtcgtc cgtacattac tggtacgctc ctgcgctcag cggtgattcg
     4801 ctccgcggag aacctgctga cactgagtga cggtaagatc tccgagaaaa cctgctgccc
     4861 ggggaaattc gacacggaag ataaggaccg tttactccag ctgcgtcaac gctccacgct
     4921 gcgttggaca gacaagaatc cgtgtcccga caatgcggaa acgtattgtc cgttctgtga
     4981 gctcctcggc cgctccggaa atgacggtaa gaaagcagaa aagaaggact ggcgcttccg
     5041 catccatttt ggtaaccttt ccctgccggg caagcccgac ttcgacggtc cgaaggcaat
     5101 tggttcacag cgcgtattaa atcgtgtgga ttttaagtcg ggaaaggcac atgacttctt
     5161 caaggcttat gaggtggacc atacccgctt ccctcgcttt gaaggggaga tcaccatcga
     5221 caataaagtg tccgccgagg cacgtaagtt gctgtgtgac agccttaaat tcacggatcg
     5281 cctgtgtgga gccctgtgcg tcatccgctt cgacgagtat acaccggccg cggacagcgg
     5341 taagcaaacc gagaatgtgc aggcagagcc caacgcaaac ctggcggaga aaactgccga
     5401 gcaaattatc tcgatcctcg atgacaacaa gaaaacggaa tacacccgcc ttttagcgga
     5461 cgccatccgc tcattgcgtc gctcctcaaa acttgttgca ggtttgccca aggatcacga
     5521 tggtaaggac gatcattact tgtgggacat tgggaagaag aagaaggatg agaattcggt
     5581 taccatccgt cagattctta ccacgagtgc cgacacgaag gagctgaaga acgctggtaa
     5641 gtggcgcgag ttttgtgaga agcttggtga ggcgctttat ttgaagagta aggatatgag
     5701 cggcgggttg aagattacac gccgtattct cggggacgca gagtttcatg gcaagccaga
     5761 tcgcttggaa aagtcccgta gtgtctcgat cggaagcgtg ttaaaggaaa ccgtggtgtg
     5821 cggagaactc gtcgcaaaga ccccgttctt ctttggcgcg atcgacgagg atgcaaaaca
     5881 aactgactta caggtactgc ttactccgga caataagtat cgcctgccgc gcagcgccgt
     5941 ccgcggcatc cttcgccgcg atcttcaaac gtactttgac agtccctgta atgcggagtt
     6001 aggcggtcgt ccatgtatgt gcaagacctg tcgtatcatg cgtggcatta cggttatgga
     6061 cgcgcgctcc gagtataacg cgcccccgga gattcgtcac cgtacccgca ttaatccgtt
     6121 tacgggaaca gttgctgagg gcgccctgtt taatatggaa gtggccccgg aagggatcgt
     6181 attcccattc caattacgct atcgcggctc tgaggacggg ctcccagacg cgctcaagac
     6241 cgtgctgaag tggtgggcag aggggcaagc ttttatgtca ggggccgcca gtaccgggaa
     6301 aggccgtttt cgtatggaga atgccaaata tgaaaccttg gatctgagcg atgagaatca
     6361 acgcaatgac tacttgaaga attggggctg gcgtgacgag aagggtcttg aggagcttaa
     6421 gaaacgttta aattcaggtc tgccggagcc tggcaattac cgtgacccga agtggcatga
     6481 aatcaacgtg tcaatcgaga tggcgagtcc cttcatcaac ggcgacccta tccgcgcagc
     6541 tgtagacaag cgtgggacgg acgtcgtgac cttcgttaag tacaaggcgg aaggggaaga
     6601 agcaaagccc gtgtgcgcat ataaggcgga gtcgtttcgc ggcgtcattc gttcggctgt
     6661 agcacgcatt cacatggaag atggagtgcc cctgactgag cttactcact cagattgcga
     6721 gtgtttgctc tgtcaaatct tcgggtcgga atatgaggct ggtaagattc gtttcgaaga
     6781 tttagtattt gagagtgacc cggagccggt caccttcgat cacgttgcaa tcgatcgctt
     6841 caccggcggt gccgctgaca agaagaagtt cgacgacagt cctttgccag ggtcgccggc
     6901 gcgccctctg atgctgaagg gttccttctg gattcgccgt gacgttctcg aagatgagga
     6961 gtattgcaaa gcgctcggga aagcattagc cgatgtaaat aatggtttat atcctctggg
     7021 tggaaaatcc gccatcggat acggacaagt taagagcctg ggtatcaagg gcgacgataa
     7081 gcgtatcagc cgtttaatga atccggcctt cgacgaaacc gacgtggcgg ttccagagaa
     7141 gcctaaaacc gatgccgagg tccgtatcga agctgagaag gtatattatc cccactactt
     7201 tgtggaaccg cacaagaagg tagagcgcga ggagaagccg tgcggtcatc aaaagtttca
     7261 tgagggacgc cttaccggta agattcgttg taaacttatt acgaaaacgc ctctcattgt
     7321 gccggacacc agcaatgatg acttctttcg ccctgcggac aaagaggccc gcaaggagaa
     7381 ggacgagtat cacaaatcct atgccttctt tcgcctgcat aagcaaatca tgatccccgg
     7441 tagtgaactc cgcgggatgg tgtcctctgt ttacgaaacg gttacaaatt catgtttccg
     7501 tatctttgac gagactaagc gtcttagttg gcgcatggat gccgatcacc aaaacgtttt
     7561 acaagatttc ttaccgggcc gtgttacggc cgacggcaag cacattcaaa agttttccga
     7621 gactgcacgt gtaccgtttt atgacaaaac gcaaaagcac tttgacattc tcgacgagca
     7681 ggaaattgcc ggtgaaaagc cggtccgcat gtgggtgaag cgtttcatta agcgcctgag
     7741 cctggtggac cctgcaaagc acccacaaaa gaagcaagac aataagtgga aacgccgcaa
     7801 ggaaggtatt gcgacgttca ttgagcagaa gaatgggtct tactatttta acgtggtcac
     7861 caacaatggc tgcaccagct ttcacttgtg gcacaagcca gacaacttcg accaagagaa
     7921 gttagagggc atccaaaacg gggagaagtt agattgctgg gttcgtgata gccgttacca
     7981 aaaggcattt caagaaatcc cggagaacga tccggacgga tgggagtgta aggaaggtta
     8041 tctgcatgtg gtgggccctt cgaaggttga atttagcgac aagaagggcg atgtcattaa
     8101 taacttccaa gggactctcc catcagtgcc caatgattgg aaaacgattc gtacaaacga
     8161 ctttaagaac cgcaaacgta agaacgagcc cgttttctgt tgcgaggatg acaaggggaa
     8221 ctactacacg atggccaaat attgtgaaac gttcttcttc gacctgaaag agaacgagga
     8281 gtatgagatc cccgagaagg cacgtatcaa gtacaaggag ttattacgcg tgtacaacaa
     8341 caatccgcaa gcggttcccg agagcgtgtt ccagagtcgc gtagcgcgcg agaacgtaga
     8401 aaagctgaag tctggggatt tggtgtattt caagcacaac gagaaatatg ttgaggacat
     8461 cgtaccggtt cgtatttcac gtaccgtaga cgaccgcatg atcggaaagc gtatgtcggc
     8521 tgatttgcgt ccatgtcacg gtgactgggt agaggacggg gacctctctg cgttgaatgc
     8581 atatccagag aagcgccttt tattgcgtca ccctaagggc ctttgcccgg catgtcgcct
     8641 tttcggaacg ggtagctaca agggccgcgt tcgttttggc ttcgcgagcc tggagaacga
     8701 cccggagtgg ttgattcctg gaaagaatcc cggtgatcca ttccacggcg ggccagtgat
     8761 gctgagcttg ctggagcgcc cgcgtcccac ctggagcatt cctggcagtg ataataagtt
     8821 caaagttcca ggccgtaagt tctacgttca ccatcatgct tggaaaacga ttaaggacgg
     8881 taaccatccg accaccggca aagccattga acaatcgcca aataaccgca cggttgaagc
     8941 gctcgccggt ggcaacagct tcagcttcga aattgcgttc gaaaatttga aggagtggga
     9001 gttgggcctg ctgatccata gcctgcagct tgaaaagggc ttggcccata agctcggtat
     9061 ggctaagagc atgggctttg gctcagtaga gatcgacgtt gagagtgtcc gcctgcgtaa
     9121 agattggaag cagtggcgca atggtaattc agagatccca aattggttgg gcaaggggtt
     9181 tgcaaagctg aaggagtggt tccgcgacga gctggacttc atcgagaatt tgaagaaatt
     9241 actgtggttc cccgaaggtg atcaagctcc gcgcgtatgt taccccatgc ttcgcaagaa
     9301 ggatgaccct aatggaaact caggttatga ggagttgaaa gacggcgaat ttaagaagga
     9361 agatcgtcag aagaagctta caacaccatg gacgccgtgg gcttgagcgg ccgcactcga
     9421 ggcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
     9481 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata
     9541 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
     9601 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
     9661 tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca
     9721 tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     9781 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     9841 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     9901 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     9961 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    10021 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    10081 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    10141 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
    10201 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    10261 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    10321 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    10381 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    10441 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
    10501 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    10561 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    10621 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    10681 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    10741 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    10801 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    10861 gatctaggtg aagatccttt ttgataatc
//
