LOCUS       sequence-344938-        7671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes HvsCas7-11 mammalian expression casette in AAV backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Ozcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, 
            Koonin EV, Abudayyeh OO, Gootenberg JS
  TITLE     Programmable RNA targeting with the single-protein CRISPR effector 
            Cas7-11.
  JOURNAL   Nature. 2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 
            10.1038/s41586-021-03886-5.
  PUBMED    34489594
REFERENCE   2  (bases 1 to 7671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 
            10.1038/s41586-021-03886-5."
COMMENT     Sequence Label: pDF0336 pAAV EFS-HvsCas7-11 for dual vector
FEATURES             Location/Qualifiers
     source          1..7671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1932..2387)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2069..2090)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2281..2300
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2459..2588
                     /label=AAV2 ITR
     promoter        2632..2843
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     polyA_signal    7438..7486
                     /note="synthetic polyadenylation signal"
     repeat_region   7487..7616
                     /label=AAV2 ITR
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
     2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     2581 gggttcctta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc
     2641 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga tccggtgcct
     2701 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     2761 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     2821 acgggtttgc cgccagaaca cagggccacc atgaagtcca tccctataac actgactttt
     2881 ctggaaccct acaggatact gccctgggcc gaaaagggga aacgagataa aaaggagtac
     2941 ctgcgaggtg ccaattatgt tcggttgcat aaggataaaa atggaaagtt caagccctac
     3001 ataacaggta cattaatccg gagcgccgtg ctgtcagcaa tcgagatgtt gctggacatc
     3061 acaaacggcg aatggaacgg caaagaatgt tgcttggcaa aattccacac tgagggtgag
     3121 aagcccagct ttctgcggaa gaagcccatt tacatccgcg ctgaaaaaga cgagatttgc
     3181 acctcccggg aaacggcatg tccactgtgc ctcatactcg ggcggtttga caaagcggaa
     3241 aaaaaggaga aagataaaga aaaatttgat gtgcacttca gcaatcttaa tttatatagt
     3301 tctaaggagt tcagtactat cgaagagctg gctcctaagc gggcactcaa tcggattgag
     3361 cagtacactg gaaaggccca agattacttc acagtatatg aagccctgaa taaagagttt
     3421 tggacattta agggacggat ccggattaaa gaagatatct acgacaaggt gacagatctg
     3481 cttttttctg cactgaggtg cgttgagaaa attgccggcg cactttgtcg gattgagatt
     3541 gataaagaac ccagccagca gaaggggttc gtgaaacgcc agcttagtaa gcaggccaaa
     3601 gaagatattg aaaagatatt tcaggtggtt aaagacgctc agaagctcag actgctgagt
     3661 gactgtttta gagagttaac aagaatggca aacaaagatg agctggcttt gccattaggc
     3721 ccagaggacg acggacatta tctgtgggat aagattaaag ttgagggaaa gacactgcgc
     3781 atattcttgc ggaattgttt tagtcaatat aaggacaatt ggttatgttt ttgcgacgaa
     3841 gccagtaaaa agggctacca gaagtacaga gagaagagac acaaactgac ggatagagaa
     3901 ctgccaactg ccactccaaa acacttcgcc gaaaaaaaag atccacagat atctccaatc
     3961 tatattgaca aggatgacaa ggtttatgag tggatcatag tcggcaggct catcgctcaa
     4021 acaccttttc atttcggcga tgaagagaaa gccgagggcg caattctcct cactccagat
     4081 aatcggttca ggctgccacg aacagcactg aggggaatcc tgagaaggga cttaaaactc
     4141 gccggtgcat cagcctgtga ggtggaagtg gggcggagtg agccatgccc ttgtgacgtg
     4201 tgtaagatta tgcgccgcgt gacactgttg gataccgtgt ctgaggacct tcgggatttc
     4261 ctgccagagt tacggaaaag aattcggatc aatccgcaga gcggcactgt ggcagagggc
     4321 gcgctgttcg acacagaagt gggacccgag ggtttgtcct ttcccttcgt gctcagatac
     4381 aagtgcgaga aactgcccga cagcttgact acagtcctgt gttggtggca ggaagggttg
     4441 gctttccttt ccggagagag tgcgacaggg aaaggtcggt ttagattaga gattaacggc
     4501 gcttttgtat gggatctcca gaagggcttg tttaactata taaaaaacca cggatttcgg
     4561 ggcgaagaaa ggttattcct ggaaggtaat gaggccgaac tcgaaaaaat ggggatccag
     4621 atcaataccg aattgttgca accagaaatg atcaagaagg aaaaaaattt taccgatttc
     4681 ccctatgact taattaagta ccagctgaac atttccagcc ctctccttct taacgaccca
     4741 attagggcta ttgctttgta cgagggggaa gggaaagccc cagacgcagt gttcttcaaa
     4801 aagtacgtct ttgaaaatgg aaagattgag gaaaagccat gttttaaagc tgagtccatc
     4861 aggggaatct tcaggaccgc agtgggacgg atcaagaatg tgttaactaa gaatcacgag
     4921 gattgcattt gtgtactgtg ccacctgttc ggcaatgtgc atgaaactgg gagattaaag
     4981 tttgaagatc ttaaaatcgt ctccggccag gaggagaaat tttttgacca cgtggcaatt
     5041 gaccgcttct tggggggcgc aaaagagaaa tacaagttcg acgataaacc catcatcggg
     5101 gcacccgaca cccctatcgt cctggagggc aagatctggg tcaagaaaga tatcaatgat
     5161 gaagcaaaag agacactgag ccaggccttc tcagatataa atactggcat ttactacctg
     5221 ggggcaaacg gctctatcgg ttacggctgg atcgaagagg ttaaggctct caaggctccc
     5281 tcctggctta agataaagga aaagcctaat ttcgagaagg atactagttt gaatattagt
     5341 gccattatga atgaattcaa gaaagacatt cagacactga atttggataa gacatatctg
     5401 ccgtatggct tcctcaaatt actcgagaag gttaaacgta cctcaagtcc tattactcac
     5461 gagcgcttct acgaaaacca cttaactgga ttcatcgagt gtagtctgaa agtcctgtct
     5521 cctctgatta ttcccgacac cgaaacaccc gagaaggaag aaaatggaca caagtactat
     5581 cacttcctca aaatagacaa caagcctatc attccgggcg ccgagattag aggtgctgtg
     5641 tcatctattt acgaggctct tactaattca tgttttcgtg tgttcggtga aaagaaagtc
     5701 ctgtcctggc ggatggaagg taaggatgca aaggaattta tgcctggcag agtgtccaag
     5761 aagaaaggta agttgtacat ggtaaagatg caggccctgc gcttaccggt atacgacaac
     5821 ccggccctgg ccaacgaaat tcggtctggg tcaatctacg agaagtacaa gaactccaaa
     5881 gtggaaatca ttttctttca aacagttgaa gggattcgaa agttcctccg cggaaatttc
     5941 aataatgtcg agtggaagaa agtattggtc actggtattg accctctcgc aattttgccc
     6001 tcccagaaaa tccccggcaa tgacaagtgg gttaagaacc tccagagcaa aatttccccc
     6061 gtccgggggt acttcaaatt taccgggcca aacaagatcg aaacaaagcg cagggaggaa
     6121 gagaaggatg agaaattaag aaccaaagct aacaaggtct cttgcctgca gaaggacaaa
     6181 tggtatgaag ctatgcacaa ccacgtggaa tataagcagg attatacacc ccctaacagc
     6241 cctaaaaccg aacccctgga acggcctaga aatatcccat gcttcgtgtg cagcgataag
     6301 gagaagatat acagaatgac caagcggtgc gagagggtct ttgtgagcct gggggagaac
     6361 gcacctaagt atgagatccc catctcagct attaagcgct atgaagtgat cctgtcagcc
     6421 taccgcgaga actgggagag aaataaaacc ccagaactgt ttcgcaccag attgccaggg
     6481 gatgggcgga ctttgaatga agatgatctg gtgtatttta gggcagacga aaacgagaaa
     6541 gtgaaggata tcatccccgt ttgtattagt aggatcgtcg acgaggtccc tctcatcaaa
     6601 aggctgtccc aagagctgtg gccctgcgtg ctggctgaat gccccctgct gggctttgag
     6661 tgcaagaagt gcgagctcga gggactgcct gagaagatct ggttccgtat aaacaaggat
     6721 gggctctgtc ctgcatgcag actcttcgga actcagatct acaaatccag agtgcggttt
     6781 tccttcgcct acgctaagaa ttggaagttc tacgacggat acattacact ccctcggctg
     6841 gagtctccac gcgcaacctg gctgattctt aaagagaagg ataagcacta catcaagtac
     6901 aaggtttgtg gtagaaagtt ctacctgcac aactcaacat atgaggatat catcaacaat
     6961 agcaagaagg agaaggagaa gaagaccgag aataatgcct cttttgaggt gctcaaggag
     7021 ggtgaattca cattcaaggt ttacttcgaa aacttagaga actgggagtt aggtctgctt
     7081 ttgctgtcct taacagggct cggcgaggct atcaagatag gccacgctaa acctctcggt
     7141 ttcgggtctg tgaagataga ggccaaaaag atctactttc gagaagaggc cgggaagttc
     7201 cacccttgcg agaaagcaga cgaatacctt aagaagggct tgaacaagct gaccagctgg
     7261 ttcggtaaga acgaaatcaa tgagcacatg cgaaacttac tcttgttcat gacttactat
     7321 cagaaccttc ccaaggttaa gtaccctgat tttgacggat acgcaaaatg gcggtgcagc
     7381 tacgttgaac aggacaaggt ggaatacttt cagaaccgct ggattgtggc ctcttaaaat
     7441 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgagga acccctagtg
     7501 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     7561 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
     7621 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc t
//
