LOCUS       sequence-34494-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  TITLE     SGC Q3
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MPP7
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..97
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             400..417
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(470..488)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      484..531
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(655..674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca
       61 tcatcatcac agcagcggcc tggttccgcg tggtagtgac tcagtaaaaa taatccgtct
      121 ggtcaaaaat agagaaccac tgggagctac cattaagaag gatgaacaga ccggggcgat
      181 cattgtggcc agaatcatga gaggaggagc tgcagataga agtggtctta ttcatgttgg
      241 tgatgaactt agggaagtca acgggatacc agtggaggat aaaaggcctg aggaaataat
      301 acagattttg gctcagtctc agggagcaat tacatttaag attatacccg gcagcaaaga
      361 ggagacacca tgatgacgaa gcttgcggcc gcactcgagc accaccacca ccaccactga
      421 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
      481 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
      541 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
      601 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      661 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      721 gggggctccc tttagggttc cgatttagtg ctttacggca cctcga
//
