LOCUS       sequence-34495-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  TITLE     SGC Q3
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ARF1
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..90
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gaataatttt gtttaacttt aagaaggaga tataccatgc atcatcatca tcatcacagc
       61 agcggcagag aaaacttgta tttccagggc atggccagcc caagaaccag gaaggttctt
      121 aaagaagtca gggtgcagga tgagaacaac gtttgttttg agtgtggcgc gttcaatcct
      181 cagtgggtca gtgtgaccta cggcatctgg atctgcctgg agtgctcggg gagacaccgc
      241 gggcttgggg ttcacctcag ctttgtgcgc tctgttacta tggacaagtg gaaggacatt
      301 gagcttgaga agatgaaagc tggtgggaat gctaagttcc gagagttcct ggagtctcag
      361 gaggattacg atccttgctg gtccttgcag gagaagtaca acagcagagc cgcggccctc
      421 tttagggata aggtggtcgc tctggccgaa ggcagagagt ggtctctgga gtcatcacct
      481 gcccagaact ggaccccacc tcagcccagg ggcctttttg gcaaaaaaga aatgcgcatc
      541 ctcatggtgg gcctggatgc tgcagggaag accacgatcc tctacaagct taagctgggt
      601 gagatcgtga ccaccattcc caccataggc ttcaacgtgg aaaccgtgga gtacaagaac
      661 atcagcttca ctgtgtggga cgtgggtggc caggacaaga tccggcccct gtggcgccac
      721 tacttccaga acacacaagg cctgatcttc gtggtggaca gcaatgacag agagcgtgtg
      781 aacgaggccc gtgaggagct catgaggatg ctggccgagg acgagct
//
