LOCUS       sequence-34496-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  TITLE     SGC Q3
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-CDPK3
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..91
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      718..765
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaaataattt tgtttaactt taagaaggag atataccatg catcatcatc atcatcacag
       61 cagcggcaga gaaaacttgt atttccaggg caaaattgac atccacgtgc tggaaaactt
      121 taaaaattat gcactgatgc tgcgcttcca gaaactggcc atgaccatta tcgcacagca
      181 aagcaacgac tacgatgttc aaaaactgaa agcggccttc ctgcatctgg atgaagaagg
      241 caaaggtaat attaccaaac tgcagctgcg taaaggcctg gaacgctcag gtctgatgct
      301 gccgccgaac tttgatctgc tgctggacca aattgactca gatggctcgg gtaatatcga
      361 ttataccgaa tttctggcag ctgcgattga ccgtcgccag ctgagcaaaa aactgatcta
      421 ctgtgcgttt cgcgtcttcg acgtggataa tgacggtgaa atcaccacgg ctgaactggc
      481 gcacgttctg tttaacggca ataaacgtgg taacattacc gaacgcgatg ttaatcaggt
      541 gaagaaaatg atccgtgaag tggataaaaa tggtgatggc aaaatcgact tctatgaatt
      601 tagtgaaatg atgaaactga cgctgtgatg acgaagcttg cggccgcact cgaggatccg
      661 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga ncaataacta
      721 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      781 atatccggat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag
      841 catcca
//
