LOCUS       sequence-34497-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  TITLE     SGC Q3
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HLTF
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..96
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             486..503
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(556..574)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      570..617
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(741..760)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tagaaataat tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat
       61 catcatcaca gcagcggcct ggttccgcgt ggtagtgatg aagaagtaga ttccgtttta
      121 tttggaagtt tgagaggtca tgtggttgga ctacgctatt acacgggagt agttaataat
      181 aatgaaatgg ttgcattaca acgagatcct aataaccctt atgataagaa tgcaattaaa
      241 gtaaacaatg tgaatggaaa tcaagttggc catttaaaga aagagcttgc aggtgctttg
      301 gcctatatca tggacaacaa attggcacaa attgaagggg tagttccttt tggtgcaaac
      361 aatgctttta ccatgcctct gcatatgact ttttggggaa aagaagaaaa tagaaaagcg
      421 gtttcagatc agttgaagaa acatggattt aaattgtgat gacgaagctt gcggccgcac
      481 tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg
      541 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
      601 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc
      661 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
      721 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
      781 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
      841 acggcacctc ga
//
