LOCUS       sequence-34498-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  TITLE     SGC Q3
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-HSP90 (3O6O)
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..83
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(801..819)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacca tgcatcatca tcatcatcac agcagcggca
       61 gagaaaactt gtatttccag ggcatgacgg agacattcgc attccaggct gaaatcaatc
      121 agctaatgtc ccttattatt aataccttct attcaaacaa ggaaattttt cttcgcgagc
      181 taattagtaa ttcgtcagat gcgtgcgaca aaatccggta ccagagtctg acgaaccagt
      241 ccgtgctcgg agatgaaccc catttgcgca ttcgcgtaat ccccgaccga gtaaataaga
      301 cactgacggt ggaggacagt ggcatcggta tgacgaaagc cgacctggtg aacaaccttg
      361 gtacgattgc tcgctccggt accaagtcat tcatggaggc attggaggcc ggtggtgata
      421 tgagcatgat cgggcagttc ggtgtcggat tttactctgc ctaccttgtc gcagaccgcg
      481 taaccgttgt gtcgaagaac aacgaggacg atgcctacac gtgggaatcc tctgcaggtg
      541 gtaccttcac tgtaacctcc acgcccgact gtgacctgaa gcgcggtacg cgtatcgtct
      601 tgcatttgaa ggaagaccag caggaatacc tggaggagcg gcggctgaag gacctcatca
      661 aaaaacactc cgaatttatc ggctacgaca tcgagttaat ggtggagaac accactgaga
      721 agtgatgacg aagcttgcgg ccgcactcga ggatccggct gctaacaaag cccgaaagga
      781 agctgagttg gctgctgcca ccgctgagca ataactagca t
//
