LOCUS       sequence-34499-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  TITLE     SGC Q3
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PHF13 (3O70)
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             10..32
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..92
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             272..289
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(342..360)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      356..403
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(527..546)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     737..758
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agaaataatt ttgtttaact ttaagaagga gatataccat gcatcatcat catcatcaca
       61 gcagcggcag agaaaacttg tatttccagg gcctcgtgac ctgcttctgc atgaagccat
      121 ttgccggccg ccccatgatc gagtgtaatg agtgccacac ctggattcac ctgtcctgtg
      181 cgaaaatccg gaaatccaat gttccagaag tgtttgtctg ccaaaagtgc cgggactcca
      241 agtgatgacg aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc
      301 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      361 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      421 atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      481 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      541 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
      601 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
      661 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
      721 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
      781 gtctattc
//
