LOCUS       sequence-344991-        1262 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation 
            domain constructs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Appling FD, Berlow RB, Stanfield RL, Dyson HJ, Wright PE
  TITLE     The molecular basis of allostery in a facilitated dissociation 
            process.
  JOURNAL   Structure. 2021 Sep 11. pii: S0969-2126(21)00261-6. doi: 
            10.1016/j.str.2021.07.011.
  PUBMED    34520739
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Structure. 
            2021 Sep 11. pii: S0969-2126(21)00261-6. doi: 
            10.1016/j.str.2021.07.011."
COMMENT     Sequence Label: HisGB1-Fusion + CBP TAZ1(340-439)
FEATURES             Location/Qualifiers
     source          1..1262
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             26..48
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             61..78
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..255
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             280..297
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     RBS             519..541
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             876..893
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(946..964)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      960..1007
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgacggtaat tccctctaga ataattttgt ttaactttaa gaaggagata tacgatgggc
       61 catcatcatc atcatcacag cagcggcatg acttacaaac tgatccttaa cggtaaaact
      121 ttgaaaggcg aaaccactac cgaagctgtt gatgctgcga ctgcagaaaa agttttcaaa
      181 cagtacgcta acgacaacgg tgttgacggt gaatggactt acgacgatgc gactaagacc
      241 ttcaccgtta ctgaagaatt cagcagcggc agcagcggcc tggttccgcg tggttcccat
      301 atgtccaatg tcatagacac tgatttcatc gacgaggaag ttcttatgtc cttggtgata
      361 gaaatgggtt tggaccgcat caaggagtta ccagaactga ccagttatga ttgtgaagtt
      421 aatgctccta tacaaggcag cagaaaccta ctgcagggtg aagaattact cagagctttg
      481 gatcaagtta actgataggg atcccctcta gaaataattt tgtttaactt taagaaggag
      541 atataccatg gcaacaggcc ccacagcaga ccctgaaaaa cgcaaactga tacagcagca
      601 gctggttcta ctgcttcatg cccacaaatg tcagagacga gagcaagcaa atggagaggt
      661 tcgagcctgt tctctcccac actgtcgaac catgaaaaac gttttgaatc acatgacaca
      721 ttgtcaggct gggaaagcct gccaagttgc ccattgtgca tcttcacgac aaatcatctc
      781 tcattggaag aactgcacac gacatgactg tcctgtttgc ctccctttga aaaatgccag
      841 tgacaagcga tagtaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc
      901 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
      961 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaggagaact
     1021 atatccggat tggcgatgga cgcgccctgt agcggcgcat taagcgcggc ggggtgtggt
     1081 gttacgcgca gcgtgacgct acacttgcag cgcctagcgc ccgctctttc gctttcttcc
     1141 cttctttctc gccacgtcgc ngctttcccc gtcaagctct aatcggggct ccnttaggnt
     1201 ccgattatgc tacggacctc gaaccaaact gataggggta atgtttcacg gtaaggggca
     1261 at
//
