LOCUS       sequence-344995-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation 
            domain constructs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Appling FD, Berlow RB, Stanfield RL, Dyson HJ, Wright PE
  TITLE     The molecular basis of allostery in a facilitated dissociation 
            process.
  JOURNAL   Structure. 2021 Sep 11. pii: S0969-2126(21)00261-6. doi: 
            10.1016/j.str.2021.07.011.
  PUBMED    34520739
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Structure. 
            2021 Sep 11. pii: S0969-2126(21)00261-6. doi: 
            10.1016/j.str.2021.07.011."
COMMENT     Sequence Label: HisGB1-FusionL21A + CBP TAZ1(340-439)
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     77..96
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        77..95
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    96..120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             135..157
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             170..187
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             203..364
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             389..406
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     RBS             628..650
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             985..1002
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1055..1073)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gcaacggcac tgtggcgccg gtgatgccgg cacgatgcgt ccggcgtaga ggatcgagat
       61 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc
      121 cctctagaaa taattttgtt taactttaag aaggagatat acgatgggcc atcatcatca
      181 tcatcacagc agcggcatga cttacaaact gatccttaac ggtaaaactt tgaaaggcga
      241 aaccactacc gaagctgttg atgctgcgac tgcagaaaaa gttttcaaac agtacgctaa
      301 cgacaacggt gttgacggtg aatggactta cgacgatgcg actaagacct tcaccgttac
      361 tgaagaattc agcagcggca gcagcggcct ggttccgcgt ggttcccata tgtccaatgt
      421 catagacact gatttcatcg acgaggaagt tcttatgtcc gcggtgatag aaatgggttt
      481 ggaccgcatc aaggagttac cagaactgac cagttatgat tgtgaagtta atgctcctat
      541 acaaggcagc agaaacctac tgcagggtga agaattactc agagctttgg atcaagttaa
      601 ctgataggga tcccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg
      661 caacaggccc cacagcagac cctgaaaaac gcaaactgat acagcagcag ctggttctac
      721 tgcttcatgc ccacaaatgt cagagacgag agcaagcaaa tggagaggtt cgagcctgtt
      781 ctctcccaca ctgtcgaacc atgaaaaacg ttttgaatca catgacacat tgtcaggctg
      841 ggaaagcctg ccaagttgcc cattgtgcat cttcacgaca aatcatctct cattggaaga
      901 actgcacacg acatgactgt cctgtttgcc tccctttgaa aaatgccagt gacaagcgat
      961 agtaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac
     1021 aaagcccgaa aggaagctga gttsgctgct gccaccgctg agcaataact agcataaccc
     1081 cttggggcct ctaaacgggt
//
