LOCUS       sequence-344998-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation 
            domain constructs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Appling FD, Berlow RB, Stanfield RL, Dyson HJ, Wright PE
  TITLE     The molecular basis of allostery in a facilitated dissociation 
            process.
  JOURNAL   Structure. 2021 Sep 11. pii: S0969-2126(21)00261-6. doi: 
            10.1016/j.str.2021.07.011.
  PUBMED    34520739
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Structure. 
            2021 Sep 11. pii: S0969-2126(21)00261-6. doi: 
            10.1016/j.str.2021.07.011."
COMMENT     Sequence Label: HIsGB1-FusionL63A + CBP TAZ1(340-439)
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             76..98
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             111..128
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             144..305
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             330..347
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     RBS             569..591
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             926..943
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atctcgatcc gcgaaattaa tacgactcca caatagggga atgagagcgg ataccaattc
       61 ccctctagga ataawtttgt ttaactttaa gaaggagata tacgatgggc catcatcatc
      121 atcatcacag cagcggcatg acttacaaac tgatccttaa cggtaaaact ttgaaaggcg
      181 aaaccactac cgaagctgtt gatgctgcga ctgcagaaaa agttttcaaa cagtacgcta
      241 acgacaacgg tgttgacggt gaatggactt acgacgatgc gactaagacc ttcaccgtta
      301 ctgaagaatt cagcagcggc agcagcggcc tggttccgcg tggttcccat atgtccaatg
      361 tcatagacac tgatttcatc gacgaggaag ttcttatgtc cttggtgata gaaatgggtt
      421 tggaccgcat caaggagtta ccagaactga ccagttatga ttgtgaagtt aatgctccta
      481 tacaaggcag cagaaaccta ctgcagggtg aagaattact cagagctgcg gatcaagtta
      541 actgataggg atcccctcta gaaataattt tgtttaactt taagaaggag atataccatg
      601 gcaacaggcc ccacagcaga ccctgaaaaa cgcaaactga tacagcagca gctggttcta
      661 ctgcttcatg cccacaaatg tcagagacga gagcaagcaa atggagaggt tcgagcctgt
      721 tctctcccac actgtcgaac catgaaaaac gttttgaatc acatgacaca ttgtcaggct
      781 gggaaagcct gccaagttgc ccattgtgca tcttcacgac aaatcatctc tcattggaag
      841 aactgcacac gacatgactg tcctgtttgc ctccctttga aaaatgccag tgacaagcga
      901 tagtaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
      961 caaagcccga aagagcga
//
