LOCUS       sequence-34500-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  TITLE     SGC Q3
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PHF13 (3O7A)
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             32..49
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             62..82
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             285..302
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(355..373)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      369..416
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(540..559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     750..771
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttgtttaact ttaagaagga gatataccat gcatcatcat catcatcaca gcagcggcag
       61 agaaaacttg tatttccagg gcgactcaga tgacgattcc tgggacctcg tgacctgctt
      121 ctgcatgaag ccatttgccg gccgccccat gatcgagtgt aatgagtgcc acacctggat
      181 tcacctgtcc tgtgcgaaaa tccggaaatc caatgttcca gaagtgtttg tctgccaaaa
      241 gtgccgggac tccaagtatg acgaagcttg cggccgcact cgagcaccac caccaccacc
      301 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      361 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      421 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      481 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      541 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      601 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
      661 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
      721 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
      781 caaccctatc tcggtctatt c
//
