LOCUS       sequence-34501-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  TITLE     SGC Q3
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RHEBL1A
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..40
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             53..73
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             652..669
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(722..740)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tttaagaagg agatatacca tgcatcatca tcatcatcac agcagcggca gagaaaactt
       61 gtatttccag ggcatgccgc tagtccgcta caggaaggtg gtcatcctcg gataccgctg
      121 tgtagggaag acatctttgg cacatcaatt tgtggaaggc gagttctcgg aaggctacga
      181 tcctacagtg gagaatactt acagcaagat agtgactctt ggcaaagatg agtttcacct
      241 acatttggtg gacacagcag ggcaggatga gtacagcatt ctgccctatt cattcatcat
      301 tggggtccat ggttatgtgc ttgtgtattc tgtcacctct ctgcatagct tccaagtcat
      361 tgagagtctg taccaaaagc tacatgaagg ccatgggaaa acccgggtgc cagtggttct
      421 agtggggaac aaggcagatc tctctccaga gagagaggta caggcagttg aaggaaagaa
      481 gctggcagag tcctggggtg cgacatttat ggagtcatct gctcgagaga atcagctgac
      541 tcaaggcatc ttcaccaaag tcatccagga gattgcccgt gtggagaatt cctatgggca
      601 agagcgtcgc tgccatctca tgtgatgacg aagcttgcgg ccgcactcga gcaccaccac
      661 caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      721 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtt
//
