LOCUS       sequence-345025-         266 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of sgRNAs in E. coli HT115 (scrambled targeting 
            sequences).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Fischer F, Benner C, Goyala A, Grigolon G, Vitiello D, Wu J, Zarse 
            K, Ewald CY, Ristow M
  TITLE     Ingestion of single guide RNAs induces gene overexpression and 
            extends lifespan in C. elegans via CRISPR activation.
  JOURNAL   J Biol Chem. 2022 May 27:102085. doi: 10.1016/j.jbc.2022.102085.
  PUBMED    35636511
REFERENCE   2  (bases 1 to 266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2022 May 27:102085. doi: 10.1016/j.jbc.2022.102085."
COMMENT     Sequence Label: L4440_BioBrick-SCR-sgRNA-A
FEATURES             Location/Qualifiers
     source          1..266
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_RNA        41..116
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        144..162
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_RNA        184..259
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 taatacgact cactataggg taagcgctgg gtcctactcc gttttagagc tagaaatagc
       61 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
      121 tttaggttct gttaagtaac tgataatacg actcactata ggggtcctga tcggaaggat
      181 cgtgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
      241 agtggcaccg agtcggtgct tttttt
//
