LOCUS       sequence-34503-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  TITLE     SGC Q3
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBH14
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..94
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 agaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca
       61 tcatcacagc agcggcctgg ttccgcgtgg tagtactgaa agccgaagag actggggcag
      121 gggaatggct tgtgttggtc gtacgagaga atgtactatt gtcccttcta atcattatgg
      181 acccattcct ggtattcctg ttggatcaac ttggagattt agagttcagg tgagcgaagc
      241 aggtgttcac agaccccatg ttggtggaat tcatggtcga agtaatgatg gggcttattc
      301 tcttgtactg gctggtggat ttgcggatga agtcgaccga ggtgatgagt tcacatacac
      361 tggaagcggt ggtaaaaatc ttgctggtaa caaaagaatt ggtgcacctt cagctgatca
      421 aacattaaca aacatgaaca gggcattggc cctaaactgt gatgctccat tggatgataa
      481 aattggagca gagtctcgga attggagagc tggtaagcca gtcagagtga tacgcagttt
      541 taaagggagg aagatcagca aatatgctcc tgaagaaggc aacagatatg atggcattta
      601 taaggtggtg aaatactggc cagagatttc atcaagccat ggattcttgg tttggcgcta
      661 tcttttaaga agagatgatg ttgaacctgc tccttggacc tctgaaggaa tagaacggtc
      721 aaggagatta tgtctacgtt tacagtatcc agcaggttac ccttcagata aag
//
