LOCUS       sequence-34504-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  TITLE     SGC Q3
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P100 (3OMC)
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata taccatgcat catcatcatc atcacagcag
       61 cggcagagaa aacttgtatt tccagggcgc aaagcagaag aaagagaagg tctgggccca
      121 ctatgaggag cagcccgtgg aggaggtgat gccagtgctg gaggagaagg agcgatctgc
      181 tagctacaag cccgtgtttg tgaccgagat cactgatgac ctgcacttct acgtgcagga
      241 tgtggagacc ggcacccagt tggagaagct gatggagaac atgcgcaatg acattgccag
      301 tcacccccct gtagagggct cctatgcccc ccgcagggga gagttctgca ttgccaaatt
      361 tgtagatgga gaatggtacc gtgcccgagt agagaaagtc gagtctcctg ccaaaataca
      421 tgtcttctac attgactacg gcaacagaga ggtcctgcca tccacccgcc tgggtaccct
      481 atcacctgcc ttcagcactc gggtgctgcc agctcaagcc acggagtatg ccttcgcctt
      541 catccaggtg ccccaagatg atgatgcccg cacggacgcc gtggacagcg tagttcggga
      601 tatccagaac actcagtgcc tgctcaacgt ggaacacctg agtgccggct gcccccatgt
      661 caccctgcag tttgcagatt ccaagggcga tgtggggctg ggcttggtga aggaagggct
      721 ggtcatggtg gaggtgcgca aggagaaaca gttccagaaa gtgatcacag aatacctgaa
      781 tg
//
