LOCUS       sequence-345047-       14183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the 
            ttTi5605 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14183)
  AUTHORS   Negishi T, Kitagawa S, Horii N, Tanaka Y, Haruta N, Sugimoto A, Sawa
            H, Hayashi KI, Harata M, Kanemaki MT
  TITLE     The auxin-inducible degron 2 (AID2) system enables controlled 
            protein knockdown during embryogenesis and development in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2021 Dec 2. pii: 6448232. doi: 10.1093/genetics/iyab218.
  PUBMED    34865044
REFERENCE   2  (bases 1 to 14183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2021 Dec 2. pii: 6448232. doi: 10.1093/genetics/iyab218."
COMMENT     Sequence Label: pTN4
FEATURES             Location/Qualifiers
     source          1..14183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     582..604
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..613
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        620..638
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1576..2175
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    complement(5625..5658)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6679..6703
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6736..6764)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7512..7532
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(8594..8610)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             9432..10226
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9486..9505)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10096..10115
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(10956..10989)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(11992..12010)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12031..12047)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12031..12047)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12044..12066)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12055..12071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12079..12085,12086..12103,12104..12109))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12124..12145
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12262..12279)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12433..13021)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12513..12532)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13192..13983,13984..14052))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13815..13834
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14053..14157)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttccat tcgccattca ggctgcgcaa
      481 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
      541 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
      601 aacgacggcc agtgaattgt aatacgactc actatagggc gaattggagc ttcgacgcaa
      661 gcttctcctt ctcttcacat ttctcaataa tttagctcta attgcattcg tattcttcgc
      721 aaaaatacgt attctgattg acccgatgaa atacggatgc ctagttgcat tgatcatcta
      781 cggttatact tatgggtgag gtttttcaaa tatattttga tttaatcgaa taatgaaatt
      841 tcagaactgg agttggtcca atttcgtggt ttatatcctc tgaactggta cctcaaaaac
      901 atcgtagtat tgctcaatct gtggcatatt caatgaatac tgtaatggtg gttatcagta
      961 catttacagt attacctcta tattccgtta ttggtagtta tgctttcctt attctatatt
     1021 caattccttc atttataagt atgcttatat tatttcgcta cttaccggaa accaaaggac
     1081 gagagattca tgatattgtg aatgaactta aaaaaaaatg aaaacctttg aaaacgttca
     1141 tacggccgaa attttacttg atcgagccaa ttgaactttt gttaagactt acagaaaagt
     1201 gagagatttt gttacaaaaa agtgagaaaa aagcgaaaat gaacaggaaa aagaagaaaa
     1261 atacacccga agtatcatat aaaaattaaa aagattgttt gacctggcgg aacttttttg
     1321 caaaaactct gaatatccat ggcacatgct tcgtgcaaaa caggatccaa atgaatatca
     1381 acaagagatt cttgaagacg acgagccact tgctcagagc attgcttatc tcgaatgaga
     1441 cccttgttaa aatcattttt caaacattat atgacattgt cgaaatgtcc tcctgattcc
     1501 atgatggtag caaactcact tcgtgtaagt gcaagtaaga tcagtgtttg tttcgatatc
     1561 agtctgtttc gcgaggcacc tttggtcttt tattgtcaac ttccattggt tcttccattg
     1621 tttctgttaa attaatgaat ttttcataaa ataaagacat tatacaatat aaaaatgaag
     1681 aatttattga aaataaactg ccagagagaa aaagtatgca acactcccgc cgagagtgtt
     1741 tgaaatggtg tacggtacat tttcgtgcta ggagttagat gtgcaggcag caacgagagg
     1801 gggagagatt tttttgggcc ttgtgaaatt aacgtgagtt ttctggtcat ctgactaatc
     1861 atgttggttt tttgttggtt tattttgttt ttatctttgt ttttatccag attaggaaat
     1921 ttaaatttta tgaatttata atgaggtcaa acattcagtc ccagcgtttt tcctgttctc
     1981 actgtttagt cgaattttta ttttaggctt tcaacaaatg ttctaactgt cttatttgtg
     2041 acctcacttt ttatattttt ttaattttta aaaatattag aagtttctag gataattttt
     2101 tcgactttta ttctctctac cgtccgcact cttcttactt ttaaattaaa ttgttttttt
     2161 ttcagttggg aaacactttg ctcatgcaaa agagaatcgc cttgtcgttt ccagaagaag
     2221 tactcgagca cgtcttctcc ttcatccaac tcgacaagga ccgtaactcc gtctccctcg
     2281 tctgcaagtc ctggtacgag atcgagcgtt ggtgccgtcg taaggtcttc atcggaaact
     2341 gctacgccgt ctccccagcc accgtcatcc gtcgtttccc aaaggtccgt tccgtcgagc
     2401 tcaagggaaa gccacacggc gccgacttca acctcgtccc agacggatgg ggaggatacg
     2461 tctacccatg gatcgaggcc atgtcctcct cctacacctg gctcgaggag atccgtctca
     2521 agcgtatggt cgtcaccgac gactgcctcg agctcatcgc caagtccttc aagaacttca
     2581 aggtcctcgt cctctcctcc tgcgagggat tctccaccga cggactcgcc gccatcgccg
     2641 ccacctgccg taacctcaag gtaagtttaa acatatatat actaactaac cctgattatt
     2701 taaattttca ggagctcgac ctccgtgagt ccgacgtcga cgaggtctcc ggacactggc
     2761 tctcccactt cccagacacc tacacctccc tcgtctccct caacatctcc tgcctcgcct
     2821 ccgaggtctc cttctccgcc ctcgagcgtc tcgtcacccg ttgcccaaac ctcaagtccc
     2881 tcaagctcaa ccgtgccgtc ccactcgaga agctcgccac cctcctccaa cgtgccccac
     2941 aactcgagga gctcggaacc ggaggataca ccgccgaggt ccgtccagac gtctactccg
     3001 gactctccgt cgccctctcc ggatgcaagg taagtttaaa cagttcggta ctaactaacc
     3061 atacatattt aaattttcag gagctccgtt gcctctccgg attctgggac gccgtcccag
     3121 cctacctccc agccgtctac tccgtctgct cccgtctcac caccctcaac ctctcctacg
     3181 ccaccgtcca atcctacgac ctcgtcaagc tcctctgcca atgcccaaag ctccaacgtc
     3241 tctgggtcct cgactacatc gaggacgccg gactcgaggt cctcgcctcc acctgcaagg
     3301 acctccgtga gctccgtgtc ttcccatccg agccattcgt catggagcca aacgtcgccc
     3361 tcaccgagca aggactcgtc tccgtctcca tgggatgccc aaagctcgag tccgtcctct
     3421 acttctgccg tcaaatgacc aacgccgccc tcatcaccat cgcccgtaac cgtccaaaca
     3481 tgacccgttt ccgtctctgc atcatcgagc caaaggcccc agactacctc accctcgagc
     3541 cactcgacat cggattcgga gccatcgtcg agcactgcaa ggacctccgt cgtctctccc
     3601 tctccggact cctcaccgac aaggtcttcg agtacatcgg aacctacgcc aagaagatgg
     3661 agatgctctc cgtcgccttc gccggagact ccgacctcgg actccaccac gtcctctccg
     3721 gatgcgactc cctccgtaag ctcgagatcc gtgactgccc attcggagac aaggccctcc
     3781 tcgccaacgc ctccaagctc gagaccatgc gttccctctg gatgtcctcc tgctccgtct
     3841 ccttcggagc ctgcaagctc ctcggacaaa agatgccaaa gctcaacgtc gaggtcatcg
     3901 acgagcgtgg agccccagac tcccgtccag agtcctgccc agtcgagcgt gtcttcatct
     3961 accgtaccgt cgccggacca cgtttcgaca tgccaggatt cgtctggaac atggaccaag
     4021 actccaccat gcgtttctcc cgtcaaatca tcaccaccaa cggactcgga ggatctggag
     4081 gcggttctgg cggaggttct ggtatgaact ctcttatcaa ggaaaacatg cgcatgaagg
     4141 ttgttcttga gggatctgtt aacggacatc agttcaagtg cactggagaa ggagaaggaa
     4201 atccatacat ggtatgtgat acatggaaat aggcacattc attaaatatt tgtattttca
     4261 gggaactcaa actatgcgca tcaaggttat cgaaggagga ccacttccat tcgctttcga
     4321 catccttgct acctctttca tgtacggatc tcgcactttc attaagtatc caaagggaat
     4381 tccagatttc ttcaagcagt ctttcccaga aggattcact tgggagcgcg ttacccgcta
     4441 cgaggatgga ggagttatca ccgttatgca agatacctcc cttgaggatg gatgccttgt
     4501 ttaccacgct caagttcgtg gagttaactt cccatctaac ggagctgtta tgcagaagaa
     4561 gactaaggga tgggaaccaa acactgaaat gatgtaccca gctgatggag gacttcgcgg
     4621 atacacccac atggctctta aggttgatgg aggaggacac ctttcttgct ctttcgttac
     4681 cacctaccgc tctaagaaga ctgttggaaa tattaagatg ccaggaatcc atgctgtcga
     4741 tcaccgtctt gagcgtcttg aggagtctga taacgagatg ttcgttgttc aacgtgagca
     4801 cgctgtcgct aagttcgctg gacttggagg aggataagag ctccgcatcg gccgctgtca
     4861 tcagatcgcc atctcgcgcc cgtgcctctg acttctaagt ccaattactc ttcaacatcc
     4921 ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca aaaaacttct
     4981 cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat
     5041 tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt
     5101 aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta tgttttttac
     5161 ttctgataaa tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat
     5221 gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc ctctcatgac
     5281 gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt caatcaagtg
     5341 aaagtttatg aaattaattt tcctgctttt gctttttggg gtttccccta ttgtttgtca
     5401 agatttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag
     5461 aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt
     5521 tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat
     5581 accaaacata actgtttgaa ttcccgcggc gcatcaggcg gcctataact tcgtatagca
     5641 tacattatac gaagttatta cgtagagctc ggtacctcgt acgtaatacg actcactagt
     5701 cattttatat ctgagtagta cctttgcttt aaatgtccat aaaactaatt ttataatcaa
     5761 taaaacaacg tttgtaaatc aactgagttt acaagtagag acattgaggg atactttcac
     5821 tatgctaaag tgataatcga ccaaataata acttcacttt ggtatttatt cctgtcttat
     5881 aaatgttatg tatgaattaa attcatatgc atatggctca ctctgacaat ttttaataat
     5941 cttccagatc aatattgact accgatgcgg gtggtctttt gctttgaatt ctgctgaact
     6001 ttacaccccg aacagcaatg tgtgcttcag cctaaaaaaa agtaagtgtg ttaatcagtg
     6061 cccccgattc ttcatttttt gcccctctct cccgtttcgt cggcaaaaga agagaaaata
     6121 aagataagtc tcaagatagg ttggtaatcg ctaaagtggt tgtgtggata agagtagcaa
     6181 aatggcagga agagcacttt gcgcgcacac actgtactca ttgttctgga taaaattctc
     6241 tcgttgtttg ccgtcggatg tctgcctctc tgcattgagc cggcttcttc actatcttta
     6301 gttaacctaa aatgccgttt cttttctcgt atccccacta tcccgttgag gttctctgct
     6361 ctcttcgctc cctaccgcca gcgagcaact atccgtgggg gcgccttgct cggaagatgg
     6421 gggggaagaa agaagatttt tgctatttgc acttgagaaa gagacttttc ctgcgtcgat
     6481 ggtcagagaa cagtgtgcag acacttttca gctacctaga attacaattg gatatccccg
     6541 cctcccaatc cacccaccca gggaaaaaga agggctcgcc gaaaatcaaa gttatctcca
     6601 ggctcgcgca tcccaccgag cggttgactt ctctccacca cttttcattt taaccctcga
     6661 tcgtcagaca cagaaatgac aagtttgtac aaaaaagcag gcttaatgag taaaggagaa
     6721 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
     6781 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
     6841 tttatttgca ctactggaaa actacctgtt ccatgggtaa gtttaaacat atatatacta
     6901 actaaccctg attatttaaa ttttcagcca acacttgtca ctactttctg ttatggtgtt
     6961 caatgcttct cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg
     7021 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     7081 cgtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc aggtgctgaa
     7141 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
     7201 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
     7261 atcatggcag acaaacaaaa gaatggaatc aaagttgtaa gtttaaacat gattttacta
     7321 actaactaat ctgatttaaa ttttcagaac ttcaaaatta gacacaacat tgaagatgga
     7381 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     7441 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
     7501 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
     7561 gaactataca aatagcattc gtagaattcc aactgagcgc cggtcgctac cattaccaac
     7621 ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa actgagttct
     7681 actaactaac gagtaatatt taaattttca gcatctcgcg cccgtgcctc tgacttctaa
     7741 gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt
     7801 ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct
     7861 ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa
     7921 aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt
     7981 gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg
     8041 cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc
     8101 gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt
     8161 tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcccgctt ttgctttttg
     8221 gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa
     8281 gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga
     8341 aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa
     8401 tgacagaata cattcccaat ataccaaaca taactgttta gatcttcgaa tgcatcgcgc
     8461 gcaccgtacg tctcgaggaa ttcctgcagg atatctggat ccacgaagct tcccatggtg
     8521 acgtcaccgg ttctagatac ctaggtgagc tctggtaccc tctagtcaag gccttaagtg
     8581 agtcgtatta cggactggcc gtcgttttac aggccggatg ggccatggca ccttaacatt
     8641 tctaaatttt agtttaataa aaaaatttta aaataatacg agttttgaga ctaactttca
     8701 ataaaaacca aaaaattgct tcaaaaaaga gctcgcgccg atttacgata aacacagcgc
     8761 gctacgacac tccgaaaacc gcgccgcacc attacattct ccgccccacc gcacggtttg
     8821 cggaaggcga tgaaattgct gggcctaaaa ttttaaccct ttttttcccg tttttcaact
     8881 gttttcccga ttttttcgat atttttttca ggtttttagt tagaaaagta tataaaaatt
     8941 gcattatttg tagttttcgc tcgtaatttg tgatagaaaa cggccaattt gcctagattt
     9001 gtgattctac caagtggaat ttttgggaaa atatcaatat tccacgattt ttattcaaat
     9061 cataacgttt cgaaattatc agcttaaata aaactaaaat aaataataaa atactaaaaa
     9121 ccctgaattt ctcggttctc ggttcagctt gagttcgttc tatacacgtt atcgattttt
     9181 gtcatttcgt ttaatcatgg gttttgattc attttaaata tgaaatttga tttattcctc
     9241 gtttcgaagt tggtttgtct tgtccggccg gtaaacaaat gttattaaaa aaaataataa
     9301 atttcgaaat ttttcgtaat gttccgcaat cgtgtttttc cgtttatctc gtcttccctt
     9361 ccttcaaata tactatttat tttctcaatt cacatatttt cagccagccc tgctcaacaa
     9421 gtggaataaa aatgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     9481 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     9541 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     9601 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     9661 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     9721 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     9781 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     9841 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     9901 atctggacga agagcatcag gggctcgcac cagccgaact gttcgccagg ctcaaggcgc
     9961 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
    10021 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
    10081 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
    10141 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
    10201 atcgccttct tgacgagttc ttctgagtcc aattactctt caacatccct acatgctctt
    10261 tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaacttctct taatttcttt
    10321 gttttttagc ttcttttaag ccacctctaa caatgaaatt gtgtagattc aaaaatagaa
    10381 ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa taataattct
    10441 atcccaaaat ctacacaatg ttctgtgtac acttcttatg ttttttactt ctgataaatt
    10501 tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag
    10561 tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc
    10621 tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa
    10681 attaattttc ctgcttttgc tttttggggt ttcccctatt gtttgtcaag atttcgagga
    10741 cggcgttttt cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa agatcggaag
    10801 aaggtttggg tttgaggctc agtggaaggt gagtagaagt tgataatttg aaagtggagt
    10861 agtgtctatg gggtttttgc cttaaatgac agaatacatt cccaatatac caaacataac
    10921 tgtttctcga ggtcgacagg cctgcatgcg tcgacataac ttcgtatagc atacattata
    10981 cgaagttata gttgcaggac cactgcgtaa cggtcttctg tataactaca aaaaagaata
    11041 aaaaaccgta tcacaactga gaaaaaagac aaaacttatt gaacagtgtt cctttcaaaa
    11101 aattattttg taaaaaatcg agtttttctc caaaataacg cacgcaatca aaaacaagaa
    11161 aactaacgtg ttctcctggg cgtggaactc cttatcaaga ttaccattat tgtctgtcag
    11221 aattgatatt tcaattattt catatctaga caaacccggg tgtcttgcat ttattagtac
    11281 tgtgagttta catacaaacg aggcttactc agataacata gagtcacctc taattagcaa
    11341 aaacccattt ctaattagct atttcgacca gttctaaagt caccgtaatt agaaatggtt
    11401 tttgtagctt ttctaattag aaatgacccc gagataagtg aaatatggca ttttctgggt
    11461 tttctagtaa gaaatgatcc cgcacgtagt gattccgcgt tacaagccaa attcaaatta
    11521 aaaaagtata ttacacaatt caagttacat tattttcagg aaacaatggc gaagagcaaa
    11581 gcagagattc aaaaggagga aagagacaac agatatgaac gaagacttct tgaaaagaga
    11641 aagagacatg aggtgagtaa ccatgataat aaataatcaa gtaagggcta tagatttata
    11701 aattctggag ttaacagttt cgaaaaaaag tagttttcat atgtgcgaag gaatatcaaa
    11761 aataggggaa tagcctaaac gagtgagaaa attaaatgtt tgttattttt tcaaaaatgt
    11821 ctgcagggcc gttatccttt caaatgcact ctatcaaaca tagatcatat ccatcatatc
    11881 ttatccagaa tttatatcat atccaagatt gagagcgttt caaggaaaat ctcaaacaac
    11941 catacaattt tgtagatcga caacaaggcc tccagtaccc agcttttgtt ccctttagtg
    12001 agggttaatt tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    12061 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    12121 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    12181 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    12241 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    12301 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    12361 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    12421 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    12481 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    12541 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    12601 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    12661 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    12721 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    12781 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    12841 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    12901 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    12961 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    13021 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    13081 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    13141 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    13201 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    13261 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    13321 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    13381 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    13441 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    13501 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    13561 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    13621 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    13681 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    13741 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    13801 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    13861 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    13921 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    13981 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    14041 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    14101 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    14161 atttccccga aaagtgccac ctg
//
