LOCUS       sequence-345062-        4169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-HKU1-Nucleocapsid
FEATURES             Location/Qualifiers
     source          1..4169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1480..1579)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1596..1615)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1597..1615)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1620..1636)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1620..1636)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2067..2858
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2951..3539
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3440..3459
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3693..3710
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3869..3896)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3988..4074)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4123..4145
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4137..4154
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4138..4154
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatcct cttatactcc cggtcattat gctggaagta gaagctcctc tggaaatcgt
      181 tcaggaatcc tcaagaaaac ttcttgggct gaccaatctg agcgaaatta ccaaaccttt
      241 aatagaggca gaaaaaccca acctaaattc actgtgtcta ctcaaccaca aggaaatact
      301 atcccacatt attcctggtt ctccgggatc actcaatttc aaaaaggtag agactttaaa
      361 ttttcagatg gtcaaggagt tcccattgct ttcggagtac ccccttctga agcaaaagga
      421 tattggtata gacacagccg gcgttctttt aaaacagctg atggtcaaca aaagcagttg
      481 ttaccgagat ggtatttcta ctatctcggt accggcccat atgccaatgc atcctatggt
      541 gaatccctcg aaggggtctt ctgggttgct aatcaccaag ctgacacttc tactccctcc
      601 gatgtttcgt caagggaccc tactactcaa gaagctatcc ctactaggtt tccgcctggt
      661 acgattttgc ctcaaggcta ttatgttgaa ggctcaggaa ggtctgcttc taatagtcga
      721 ccaggttcac gttctcaatc acgtggaccc aataatcgtt cattaagtag aagtaattct
      781 aattttagac attcagattc tatagtaaaa cctgatatgg ctgatgagat cgctaatctt
      841 gttttagcca agcttggtaa agattctaaa cctcagcaag tcactaagca aaatgccaag
      901 gaaatcaggc ataaaatttt aacaaaacct cgccaaaagc gaactcctaa taaacattgt
      961 aatgttcaac agtgttttgg taaaagagga ccttctcaaa attttggtaa tgctgaaatg
     1021 ttaaagcttg gtactaatga tcctcagttt cctattcttg cagaattagc tcctacacca
     1081 ggtgcttttt tctttggttc taaattagac ttggttaaaa gagattccga ggctgactca
     1141 cctgttaaag atgtttttga acttcattat tctggttcta ttaggtttga tagtacttta
     1201 ccaggctttg agacaattat gaaagttctt gaagagaatt taaatgctta cgttaattct
     1261 aatcagaaca ctgattctga ttcgttgagt tctaaacctc agcgtaaaag aggtgttaaa
     1321 caattaccag aacagtttga ctctcttaat ttaagtgctg gtactcagca catttcaaat
     1381 gattttactc ctgaggatca tagtttactt gctactcttg atgatcctta tgtagaagac
     1441 tctgttgctc ttaattaaga cgcgatcgcg gcctcatggg cccagctttc ttgtacaaag
     1501 ttggcattat aaaaaataat tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc
     1561 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
     1621 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     1681 aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt
     1741 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     1801 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     1861 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     1921 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     1981 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     2041 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc
     2101 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     2161 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     2221 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     2281 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     2341 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     2401 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     2461 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     2521 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     2581 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     2641 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     2701 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     2761 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     2821 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa
     2881 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     2941 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3001 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3061 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3121 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3181 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3241 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3301 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3361 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3421 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3481 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3541 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3601 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3661 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     3721 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     3781 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     3841 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     3901 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     3961 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     4021 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     4081 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
     4141 aaacgacggc cagtcttaag ctcgggccc
//
