LOCUS       sequence-345063-        6353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transposon allowing the expression of the fluorescent protein 
            mEos3.2 in multiple mycoplasma species, under the control of the 
            Spiralin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Rideau F, Villa A, Belzanne P, Verdier E, Hosy E, Arfi Y
  TITLE     Imaging Minimal Bacteria at the Nanoscale: a Reliable and Versatile 
            Process to Perform Single-Molecule Localization Microscopy in 
            Mycoplasmas.
  JOURNAL   Microbiol Spectr. 2022 Jun 29;10(3):e0064522. doi: 
            10.1128/spectrum.00645-22. Epub 2022 May 31.
  PUBMED    35638916
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr."; date: "2022-06-29"; volume: "10(3):e0064522. doi"; pages: 
            " 10.1128/spectrum.00645-22. Epub 2022 May 31"
COMMENT     Sequence Label: pMT85_PS-mEos3.2_GentaR
FEATURES             Location/Qualifiers
     source          1..6353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1396..1412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1401..1423
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1420..1436
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1420..1436
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1515..1537)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3964..4552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4453..4472
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4706..4723
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tataattgtg taaaaaccca tagctttgga cacacactag ttctgcagaa ttaaaagtta
       61 gtgaacaaga aaacagtgaa gcaccagttt ctgaaccaaa agaagacgaa aaaacaaaaa
      121 aagattaagc aatttatttg gaaaatcttt ttttgttttt ttaagaaata tttattgttt
      181 tttttaaaaa attattgtac agttgctact ataagggaaa gaaaaaaaga aagatataaa
      241 ttgtataaag tagggttaga agcaattaat aattattatt aatgttattt ttctcttata
      301 tattcaatgt aattttaatt acatttgctt ttaataaaaa cactacttaa tagagaaagg
      361 aaatataaga tctatgtcag ctattaaacc agatatgaaa attaaattaa gaatggaagg
      421 aaatgtaaat ggacatcatt ttgtaataga tggagatggt actggtaaac catttgaagg
      481 taaacaatca atggatttag aagttaaaga aggtggtcca ttaccatttg cttttgatat
      541 tttaactact gcttttcatt atggtaatag agtttttgct aaatatccag ataatattca
      601 agattatttt aaacaatcat ttccaaaagg ttattcatga gaaagatcat taacttttga
      661 agatggtggt atttgtaatg ctagaaatga tattactatg gaaggtgata ctttttataa
      721 taaagttaga ttttatggta ctaattttcc agctaatggt ccagttatgc aaaaaaaaac
      781 tttaaaatga gaaccatcaa ctgaaaaaat gtatgttaga gatggtgttt taactggtga
      841 tattgaaatg gctttattat tagaaggtaa tgctcattat agatgtgatt ttagaactac
      901 ttataaagct aaagaaaaag gtgttaaatt accaggtgct cattttgttg atcattgtat
      961 tgaaatttta tcacatgata aagattataa taaagttaaa ttatatgaac atgctgttgc
     1021 tcattcaggt ttaccagata atgctagaag atagcttaag aaattaaagt tggttcattc
     1081 aaagttacaa ttcgtttaaa aagtgaataa ttaattatta attaatttat taaaaaacaa
     1141 ccaaaaggtt gttttttatt ttttaaatct agtggatctc ataaaaatgt atcctaaatc
     1201 aaatatcgga caagcagtgt ctgatataac aaaaaatcga tttaatagac acattaacag
     1261 cactgttttt atgtgtgcga taatttataa tatttcggac ggttgcggat ccacccgcaa
     1321 ttactgtgag ttagctcact cattaggcac cccaggcttt acactttata cttccggctc
     1381 gtatattgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccttg
     1441 attacggaat tcacggccgg gggggccacc ccaccaattg acgcggccgc aactctagag
     1501 gattcatcgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     1561 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     1621 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
     1681 gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc
     1741 gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtgacc
     1801 tatcccatta cggtcagtcc gccgtttgtt cccacggaga atccgacggg ttgttactcg
     1861 ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat
     1921 ggctagaagc tttaggatga atggatttat tcttcaagaa aatacatcaa ttttgataag
     1981 tagaaatggt aaaaacattg tatagcattt tacacaggag tctggacttg actgagttta
     2041 tggaagaagt tttaattgat gataatatgg tttttgatat tgataattta aaaggatttc
     2101 ttaatgatac cagttcattt gggtttatag ctaaagaaaa taataaaatt ataggatttg
     2161 catattgcta tacactttta agacctgatg gaaaaacaat gttttattta cactcaatag
     2221 gaatgttacc taactatcaa gacaaaggtt atggttcaaa attattatct tttattaagg
     2281 aatattctaa agagattggt tgttctgaaa tgtttttaat aactgataaa ggtaatccta
     2341 gagcttgcca tgtatatgaa aaattaggtg gtaaaaatga ttataaagat gaaatagtat
     2401 atgtatatga ttatgaaaaa ggtgataaat aaatgaatat agttgaaaat gaaatatgta
     2461 taagaacttt aatagatgat gattttcctt tgatgttaaa atggttaact gatgaaagag
     2521 tattagaatt ttatggtggt agagataaaa aatatacatt agaatcatta aaaaaacatt
     2581 atacagagcc ttgggaagat gaagttttta gagtaattat tgaatataac aatgttccta
     2641 ttggatatgg acaaatatat aaaatgtatg atgagttata tactgattat cattatccaa
     2701 aaactgatga gatagtctat ggtatggatc aatttatagg agagccaaat tattggagta
     2761 aaggaattgg tacaagatat attaaattga tttttgaatt tttgaaaaaa gaaagaaatg
     2821 ctaatgcagt tattttagac cctcataaaa ataatccaag agcaataagg gcataccaaa
     2881 aatctggttt tagaattatt gaagatttgc cagaacatga attacacgag ggcaaaaaag
     2941 aagattgtta tttaatggaa tatagatatg atgataatgc cacaaatgtt aaggcaatga
     3001 aatatttaat tgagcattac tttgataatt tcaaagtaga tagtattgaa ataatcggta
     3061 gtggttatga tagtgtggca tatttagtta ataatgaata catttttaaa acaaaattta
     3121 gtactaataa gaaaaaaggt tatgcaaaag aaaaagcaat atataatttt ttaaatacaa
     3181 atttagaaac taatgtaaaa attcctaata ttgaatattc gtatattagt gatgaattat
     3241 ctatactagg ttataaagaa attaaaggaa cttttttaac accagaaatt tattctacta
     3301 tgtcagaaga agaacaaaat ttgttaaaac gagatattgc cagtttttta agacaaatgc
     3361 acggtttaga ttatacagat attagtgaat gtactattga taataaacaa aatgtattag
     3421 aagagtatat attgttgcgt gaaactattt ataatgattt aactgatata gaaaaagatt
     3481 atatagaaag ttttatggaa agactaaatg caacaacagt ttttgagggt aaaaagtgtt
     3541 tatgccataa tgattttagt tgtaaccatc tattgttaga tggcaataat agattaactg
     3601 gaataattga ttttggagat tctggaatta tagatgaata ttgtgatttt atatacttac
     3661 ttgaagatag tgaagaagaa ataggaacaa attttggaga agatatatta agaatgtatg
     3721 gaaatataga tattgagaaa gcaaaagaat atcaagatat agttgaagaa tattatccta
     3781 ttgaaactat tgtttatgga attaaaaata ttaaacagga atttatcgaa aatggtagaa
     3841 aagaaattta taaaaggact tataaagatt aataaagatc tacgaaggca tgaccaaaat
     3901 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3961 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4021 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4081 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4141 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4201 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4261 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4321 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4381 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4441 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4501 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4561 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4621 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4681 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     4741 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcacgc tagcggatct
     4801 cataaaaatg tatcctaaat caaatatcgg acaagcagtg tctgttataa caaaaaatcg
     4861 atttaataga cacattaaca gcactgtttt tatgtgtgcg ataatttata atatttcgga
     4921 cggttgcggt acccttttac acaattatac ggactttatc ctgcaggggc ccaattgtgt
     4981 aaaagtaaaa aggccatata acagtccttt tacggtacaa tgtttttaac gacaaaaaca
     5041 tacccaggag gacttttaca tgacccaagt acattttaca ctgaaaagcg aagagattca
     5101 aagcattatt gaatattctg taaaggatga cgtttctaaa aatattttaa caacggtatt
     5161 taatcaacta atggaaaatc aacgaacaga atatattcaa gcaaaagaat atgaacgaac
     5221 agaaaaccga caaagtcaac gaaatggcta ttatgagcgc agctttacga cacgtgtagg
     5281 cacgctagaa ttaaaagtac ccagaacacg tgatggccat ttttcaccca cagtgtttga
     5341 acgttatcaa cgaaacgaaa aagccctcat ggcttcaatg ttggaaatgt atgtatcagg
     5401 cgtttcaact cgtaaagtat caaaaattgt ggaagaactt tgtggtaaat ccgtctctaa
     5461 gtccttcgtt tctagcttaa cagaacagct agaacctatg gttaacgagt gacagaatcg
     5521 tttattatca gaaaaaaatt atccttactt aatgaccgat gtactctata taaaagtacg
     5581 agaagaaaat cgagtactct caaaaagctg tcatatagcg attggaataa ccaaagatgg
     5641 cgaccgtgaa attatcggct tcatgattca aagtggcgaa agcgaagaga cctggacaac
     5701 attttttgaa tacctaaaag aacgcggttt acaaggtacg gaactcgtta tttctgatgc
     5761 gcacaaagga ttagtctctg ccattagaaa atccttcacc aacgtaagtt ggcaaaaatg
     5821 ccaagttcac ttcctaagaa atatctttac caccattcct aaaaaaaatt caaaatcttt
     5881 cagagaagct gttaaaggaa tttttaagtt cacagatatt aacttagcgc gtgaggctaa
     5941 aaatcgattg attcatgatt atatcgatca accaaaatat tcaaaagctt gcgcatcatt
     6001 ggatgatgga ttcgaagacg cctttcaata taccgtacaa ggaaattccc acaatcgact
     6061 aaagagtacc aatctaattg aacgactgaa tcaagaagta cgcagaagag aaaagattat
     6121 tcgcatcttc cccaatcaaa catcagccaa tcgcttaatt ggagccgttc ttatggacct
     6181 acatgatgaa tggatttatt cttcaagaaa atacatcaat tttgataagt agaaatggta
     6241 aaaacattgt atagcatttt acacaggagt ctggacttga ctcacttcct ttattatttt
     6301 tcattttttt gacctcgagg gggggcccac catacagctg acgataaagt ccg
//
