LOCUS       sequence-34507-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  TITLE     SGC Q3
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-MAPK1 (3OZ6)
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             32..49
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             62..82
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttgtttaact ttaagaagga gatataccat gcatcatcat catcatcaca gcagcggcag
       61 agaaaacttg tatttccagg gcagagttga cagacatgta ttgaggaaat atgaactggt
      121 aaaaaagttg gggaagggtg cgtatggcat agtttggaaa tctattgatc gtaggactgg
      181 agaagtagtt gcagtcaaga aaatcttcga tgcatttcaa aattcaacag atgctcaaag
      241 aacatttaga gagattatga tattgacaga gctatctggt cacgagaata ttgtaaattt
      301 actaaatgtc ttaagagcag acaatgatag agatgtctac ttggtttttg actatatgga
      361 gacggaccta catgcagtca ttagagcaaa tattttggag cctgttcata aacaatatgt
      421 agtttatcaa cttattaagg taattaaata tttacatagt ggtgggttat tacatcgtga
      481 tatgaaacca tcaaatatac ttctaaatgc tgaatgccat gtaaaagtgg cagactttgg
      541 attatctaga agctttgtca acataaggcg tgttacaaat aatattccat taagtattaa
      601 cgaaaatact gaaaatttcg atgatgatca gcctattctc acagactatg ttgcaactag
      661 atggtatagg gctccagaaa tacttttagg atcaactaag tatacaaagg ggattgacat
      721 gtggtctttg ggctgtattt tgggagagat attgtgtgga aaaccaattt ttccaggttc
      781 atcaactatg aatcaacttg aaagaatcat a
//
