LOCUS       sequence-345071-       12110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The lentiviral vector for R-GECO1.0, a genetically encoded red 
            fluorescent Ca2+ sensor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12110)
  AUTHORS   Hirota K, Hirashima T, Horikawa K, Yasoda A, Matsuda M
  TITLE     C-type Natriuretic Peptide-induced PKA Activation Promotes 
            Endochondral Bone Formation in Hypertrophic Chondrocytes.
  JOURNAL   Endocrinology. 2022 Mar 1;163(3). pii: 6511000. doi: 
            10.1210/endocr/bqac005.
  PUBMED    35041746
REFERENCE   2  (bases 1 to 12110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Endocrinology."; date: "2022-03-1"; volume: "163(3). pii: 6511000. 
            doi"; pages: " 10.1210/endocr/bqac005"
COMMENT     Sequence Label: pCSIIhyg-RGECO1.0
FEATURES             Location/Qualifiers
     source          1..12110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(75..95)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        80..409
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      260..395
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     322..341
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        457..504
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             523..897
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1027..1148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1064..1083)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1118..1137
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1197..1213)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1197..1213)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1210..1232)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1221..1237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1245..1251,1252..1269,1270..1275))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1290..1311
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1428..1445)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1599..2187)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1679..1698)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2358..3149,3150..3218))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2981..3000
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3219..3323)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3398..3417)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3589..3968
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3970..4168
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4123..4143
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4186..4366
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4413..4538
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5030..5263
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5448..5492
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5766..5883
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5939..7101
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6170..7092
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7049..7069
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7181..7237
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     7933..7952
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7976..8419
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTFDFPEFLTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTDLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     misc_feature    8498..9065
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8659..8676)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8886..8905
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9104..10123
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    10159..10747
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10212..10232)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10630..10641)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11266..11446
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11472..11489)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11478..11702
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(11748..12110,1..66)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11835..11854)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12045..12066
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
       61 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
      121 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
      181 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
      241 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
      301 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
      361 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
      421 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
      481 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
      541 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
      601 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
      661 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
      721 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
      781 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
      841 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
      901 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
      961 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     1021 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1081 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1141 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     1201 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     1261 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1321 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1381 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1441 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1501 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1561 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1621 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1681 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1741 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1801 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1861 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1921 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1981 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2041 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2101 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2161 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2221 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2281 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2341 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2401 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2461 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2521 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2581 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2641 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2701 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2761 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2821 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2881 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2941 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3001 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3061 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3121 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3181 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3241 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3301 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     3361 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     3421 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     3481 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     3541 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     3601 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     3661 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     3721 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     3781 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     3841 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     3901 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     3961 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     4021 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     4081 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     4141 gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta
     4201 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4261 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4321 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     4381 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     4441 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     4501 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     4561 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     4621 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     4681 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     4741 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     4801 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     4861 ccaccgcaca gcaagggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     4921 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     4981 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     5041 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     5101 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     5161 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     5221 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     5281 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     5341 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     5401 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     5461 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     5521 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     5581 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     5641 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     5701 agagagacag agacagatcc attcgattag tgaacggatc tacaaatggc agtattcatc
     5761 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     5821 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     5881 tttcgggttt attacaggga cagcagaaat tcactttgaa ttaattcaag cttcgtgagg
     5941 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     6001 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     6061 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     6121 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
     6181 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
     6241 ttccacctgg ctccagtacg tgattcttga tcccgagctg gagccagggg cgggccttgc
     6301 gctttaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
     6361 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     6421 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     6481 gcgggccagg atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     6541 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     6601 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     6661 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     6721 tggccgcttc ccggccctgc tccagggggc tcaaaatgga ggacgcggcg ctcgggagag
     6781 cgggcgggtg agtcacccac acaaaggaaa ggggcctttc cgtcctcagc cgtcgcttca
     6841 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctg gagcttttgg
     6901 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     6961 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     7021 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     7081 cttccatttc aggtgtcgtg aacacgctac cggtctcgac gaattcggcc tcgagggcgg
     7141 cggccgcggc tctagaggat ccaccatggt cgactcatca cgtcgtaagt ggaataaggc
     7201 aggtcacgca gtcagagcta taggtcggct gagctcaccc gtggtttccg agcggatgta
     7261 ccccgaggac ggcgccctga agagcgagat caagaagggg ctgaggctga aggacggcgg
     7321 ccactacgcc gccgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     7381 cgcctacatc gtcgacatca agttggacat cgtgtcccac aacgaggact acaccatcgt
     7441 ggaacagtgc gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     7501 gggaggtaca ggcgggagtc tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     7561 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     7621 cgagggcgag ggcgagggcc gcccctacga ggcctttcag accgctaagc tgaaggtgac
     7681 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     7741 caaggcctac attaagcacc cagccgacat ccccgactac ttcaagctgt ccttccccga
     7801 gggcttcagg tgggagcgcg tgatgaactt cgaggacggc ggcattattc acgttaacca
     7861 ggactcctcc ctgcaggacg gcgtattcat ctacaaggtg aagctgcgcg gcaccaactt
     7921 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcta cgcgtgacca
     7981 actgactgaa gagcagatcg cagaatttaa agaggctttc tccctatttg acaaggacgg
     8041 ggatgggacg ataacaacca aggagctggg gacggtgatg cggtctctgg ggcagaaccc
     8101 cacagaagca gagctgcagg acatgatcaa tgaagtagat gccgacggtg acggcacatt
     8161 cgacttccct gagttcctga cgatgatggc aagaaaaatg aatgacacag acagtgaaga
     8221 ggaaattaga gaagcgttcc gcgtgtttga taaggacggc aatggctaca tcggcgcagc
     8281 agagcttcgc cacgtgatga cagaccttgg agagaagtta acagatgagg aggttgatga
     8341 aatgatcagg gtagcagaca tcgatgggga tggtcaggta aactacgaag agtttgtaca
     8401 aatgatgaca gcgaagtagg aattcctcga gggcggccgc tctagagatc cgttaactaa
     8461 cttaagctag caacggtttc cctctagcgg gatcaattcc gccccccccc cctaacgtta
     8521 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     8581 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     8641 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     8701 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     8761 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     8821 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     8881 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     8941 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     9001 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     9061 gataagcttg ccacaacccg taccaaagat ggatagatcc ggaaagcctg aactcaccgc
     9121 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     9181 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     9241 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     9301 atcggccgcg ctcccgattc cggaagtgct tgacattggg gagttcagcg agagcctgac
     9361 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     9421 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
     9481 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     9541 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     9601 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     9661 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     9721 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     9781 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     9841 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
     9901 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     9961 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
    10021 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
    10081 tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagactagag ttaacatcga
    10141 gggatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
    10201 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
    10261 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
    10321 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
    10381 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
    10441 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
    10501 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
    10561 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
    10621 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
    10681 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
    10741 tccccgcatc gataccgtcg agacctagaa aaacatggag caatcacaag tagcaataca
    10801 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
    10861 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
    10921 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
    10981 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
    11041 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
    11101 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
    11161 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
    11221 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
    11281 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    11341 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    11401 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
    11461 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
    11521 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
    11581 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
    11641 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
    11701 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
    11761 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
    11821 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
    11881 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
    11941 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
    12001 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
    12061 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
//
