LOCUS       sequence-345097-        8040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one piggyBac transposon destination vector for 
            mCMV+dox-inducible expression of Mega Gate cloned elements upstream 
            of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin 
            resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8040)
  AUTHORS   Kramme C, Plesa AM, Wang HH, Wolf B, Smela MP, Guo X, Kohman RE, 
            Chatterjee P, Church GM
  TITLE     An integrated pipeline for mammalian genetic screening.
  JOURNAL   Cell Rep Methods. 2021 Sep 27;1(6):100082. doi: 
            10.1016/j.crmeth.2021.100082. eCollection 2021 Oct 25.
  PUBMED    35474898
REFERENCE   2  (bases 1 to 8040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2021-09-27"; volume: "1(6):100082. doi"; pages: " 
            10.1016/j.crmeth.2021.100082. eCollection 2021 Oct 25"
COMMENT     Sequence Label: PB-CT3G-CERP2-MG-IRES2-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..8040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     278..302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    368..492
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(613..737)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             739..765
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(859..1433,1434..1436,1437..1445)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1026..1043)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1253..1272
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     polyA_signal    2174..2308
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   2580..2614
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2625..2642)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2625..2641)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2634..2656)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2850..2869)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2971..2993
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3032..3050)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3118..3222
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3223..3291,3292..4083)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3441..3460)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4254..4842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4743..4762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4996..5013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5219..5240
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5255..5260,5261..5278,5279..5285)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5293..5309
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5298..5320
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5317..5333
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5317..5333
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   5359..5421
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    5597..5678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6395..6451)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(6461..7204)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7205..7228)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      7228..7237
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7257..7468)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        7469..7848
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag cttgcctata taagcagagc tcgtttagtg aaccgtcaga
      301 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      361 gcctaccaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
      421 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
      481 ccagtcacta tggcgacaga agaagtatag ggataacagg gtaattgttg taagcgcgct
      541 atgatggagg ctatgccact agaatctgcg ttcgctacct taggaccgtt atagttagaa
      601 ggaaagctcc atcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
      661 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
      721 ttcttgtaca aagtggtata cccatacgat gttccagatt acgcttagta atgagaaata
      781 ctcagaagat gtcactcacc atagagagac cattaatggg tctcctaggc cactgagcaa
      841 gaactatgaa tgagatctcc cctctccctc ccccccccct aacgttactg gccgaagccg
      901 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
      961 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     1021 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     1081 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     1141 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     1201 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     1261 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     1321 tgatctgggg cctcggtaca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     1381 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
     1441 caaccatggt cagcaaaggc gaagaagata acatggcatc actgccagcg acacacgaac
     1501 ttcatatttt cggctccatc aatggtgtgg acttcgatat ggttggacag ggtactggga
     1561 accctaacga tggatatgag gagttgaact tgaaaagtac caagggcgat cttcagtttt
     1621 caccttggat tctcgtacct catataggtt atggctttca tcaatatctg ccctatcctg
     1681 acggtatgtc acctttccaa gcagctatgg ttgacgggag cggttatcag gttcaccgga
     1741 caatgcagtt tgaagatgga gcctctttga ccgtaaatta tcggtatact tatgagggca
     1801 gccatattaa aggcgaggct caagttaagg gaactgggtt ccccgcggat ggtcctgtaa
     1861 tgacaaacag tctgacggct gcagactggt gccgctctaa gaaaacatat ccaaatgaca
     1921 agacgattat tagcactttc aagtggtcat acactacagg aaatggtaaa cgctatagga
     1981 gcactgcccg gaccacgtat actttcgcga agccgatggc ggcaaattat ttgaaaaacc
     2041 aaccgatgta tgtgttcaga aagacagaac tcaagcactc taaaactgaa ctgaacttca
     2101 aagagtggca gaaagccttt actgatgtaa tgggcatgga cgaactctat aagtaagcgg
     2161 ccgctttctc cgcgatccag acatgataag atacattgat gagtttggac aaaccaaaac
     2221 tagaatgcag tgaaaaaaat gccttatttg tgaaatttgt gatgctattg ccttatttgt
     2281 aaccattata agctgcaata aacaagttat ctataacaag aaaatatata tataataagt
     2341 tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa atcttaaaag
     2401 tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt tcaaaatcag
     2461 tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga ctgagatgtc
     2521 ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc
     2581 atgcgtcaat tttacgcaga ctatctttct agggttaaga attcactggc cgtcgtttta
     2641 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     2701 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     2761 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     2821 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     2881 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     2941 tccgcttaca gacaagctgt gaccatgtct ccgggagctg catgtgtcag aggttttcac
     3001 cgtcatcacc gaaacacgcg agttcgaaag ggcctcgtga tacgcctatt tttataggtt
     3061 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     3121 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3181 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     3241 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     3301 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3361 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3421 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     3481 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     3541 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3601 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     3661 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     3721 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     3781 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3841 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3901 tggtttattg ctgataaatc tggagccggt gagcgtggtt cacgcggtat cattgcagca
     3961 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4021 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4081 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     4141 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     4201 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4261 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4321 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4381 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     4441 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4501 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4561 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4621 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4681 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4741 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4801 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4861 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4921 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     4981 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg atgagcgccc aatacgcaaa
     5041 ccgcctctca ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     5101 tggaaagcgg gcagtgagcg caacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     5161 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     5221 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     5281 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     5341 acgccaaggt cgacttaacc ctagaaagat aatcatattg tgacgtacgt taaagataat
     5401 catgcgtaaa attgacgcat gtgttttatc ggtctgtata tcgaggttta tttattaatt
     5461 tgaatagata ttaagtttta ttatatttac acttacatac taataataaa ttcaacaaac
     5521 aatttattta tgtttattta tttattaaaa aaaaacaaaa actcaaaatt tcttctataa
     5581 agtaacaaaa cttttagcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     5641 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     5701 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     5761 aatgtggtat ggctgattat gatcctctgg agattcatgc gccaggtttt ctggtcatgc
     5821 accacgttcg tgggccctca ggaacctcca cgtcagcggt aactgtgaag cccagtcgct
     5881 cgtaaaaagg aagattccgg ggagcactgg tttcgaggaa cgccggcacg ccagcccgtt
     5941 ccgcggcctc aacaccagga agaacgacag cgcttcccaa acctttacct tggtgatctg
     6001 ggctgacacc gacagtggca agaaaccacg caggctcctt tggtctatga ggagccagca
     6061 agccctccat ttgctgttgt gccgccaacc tactccctga cagctcggcc atccgcggac
     6121 ctatttccgc gaacactgcg ccggcttcga cgctttccgg agtagtccag accgcaactg
     6181 cggcaccatc gtccgcaacc caaactttac caatgtccag acccacgcgc gtaagaaaca
     6241 gctcttgcaa ctcagttacc ctctctatgt gtctgtccgg atctacggta tggcgggtcg
     6301 ctggataatc tgcaaatgct gcggcaagtg ttcggacagc ccgtggtaca tcgtcacggg
     6361 tagcgagcct aacagtgggc ttatattccg tcatggggcc ggggttctcc tccacgtcgc
     6421 cggcctgctt cagcaggctg aagttggtgg cgccgctgcc cccggggagc atgtcaaggt
     6481 caaaatcgtc aagagcgtca gcaggcagca tatcaaggtc aaagtcgtca agggcatcgg
     6541 ctgggagcat gtctaagtca aaatcgtcaa gggcgtcggt cggcccgccg ctttcgcact
     6601 ttagctgttt ctccaggcca catatgatta gttccaggcc gaaaaggaag gcaggttcgg
     6661 ctccctgccg gtcgaacagc tcaattgctt gtttcagaag tgggggcata gaatcggtgg
     6721 taggtgtctc tctttcctct tttgctactt gatgctcctg ttcctccaat acgcagccca
     6781 gtgtaaagtg gcccacggcg gacagagcgt acagtgcgtt ctccagggag aagccttgct
     6841 gacacaggaa cgcgagctga ttttccaggg tttcgtactg tttctctgtt gggcgggtgc
     6901 cgagatgcac tttagccccg tcacgatgtg agaggagagc acagcggtat gacttggcgt
     6961 tgttccgcag aaagtcttgc catgactcgc cttccagggg gcaggagtgg gtatgatgcc
     7021 tgtccagcat ctcgattggc agggcatcga gcagggcccg cttgttcttc acgtgccagt
     7081 acagggtagg ctgctcaact cccagctttt gagcgagttt ccttgtcgtc aggccttcga
     7141 taccgactcc attgagtaat tccagagcag agtttatgac tttgctcttg tccagtctag
     7201 acatcttatc gtcatcgtct ttgtaatcca tggtggcgga tcccgcgtca cgacacctgt
     7261 gttctggcgg caaacccgtt gcgaaaaaga acgttcacgg cgactactgc acttatatac
     7321 ggttctcccc caccctcggg aaaaaggcgg agccagtaca cgacatcact ttcccagttt
     7381 accccgcgcc accttctcta ggcaccggtt caattgccga cccctccccc caacttctcg
     7441 ggcactgtgg gccatgtgcg ctctgcccca tggtaatagc catgactaat acgtagatgt
     7501 actgccaagt aggaaagtgc cataaggtca tgtactgggc ataatgccag gcgggccatt
     7561 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     7621 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtg cctattggcg
     7681 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     7741 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     7801 atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcat aagctctaat
     7861 cgcataatcg agctaagagc gattaatatg cttagctagc ttagctaagc tagatttctc
     7921 tattctccca caatatatag cagctattag cttagcagtc cgaatacgat cgtccacgat
     7981 ccataagcgt aacacatttg agtagcgact gacgggcacc ggagccactc gagtggaatt
//
