LOCUS       sequence-345099-        8031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one piggyBac transposon destination vector for 
            mCMV+dox-inducible expression of Mega Gate cloned elements upstream 
            of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8031)
  AUTHORS   Kramme C, Plesa AM, Wang HH, Wolf B, Smela MP, Guo X, Kohman RE, 
            Chatterjee P, Church GM
  TITLE     An integrated pipeline for mammalian genetic screening.
  JOURNAL   Cell Rep Methods. 2021 Sep 27;1(6):100082. doi: 
            10.1016/j.crmeth.2021.100082. eCollection 2021 Oct 25.
  PUBMED    35474898
REFERENCE   2  (bases 1 to 8031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2021-09-27"; volume: "1(6):100082. doi"; pages: " 
            10.1016/j.crmeth.2021.100082. eCollection 2021 Oct 25"
COMMENT     Sequence Label: PB-CT3G-CERP2-MG-IRES2-mTagBFP2
FEATURES             Location/Qualifiers
     source          1..8031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     278..302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    368..492
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(613..737)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             739..765
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(859..1433,1434..1436,1437..1445)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1026..1043)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1253..1272
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     polyA_signal    2165..2299
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   2571..2605
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2616..2633)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2616..2632)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2625..2647)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2841..2860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2962..2984
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3023..3041)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3109..3213
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3214..3282,3283..4074)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3432..3451)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4245..4833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4734..4753
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4987..5004
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5210..5231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5246..5251,5252..5269,5270..5276)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5284..5300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5289..5311
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5308..5324
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5308..5324
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   5350..5412
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    5588..5669
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6386..6442)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(6452..7195)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7196..7219)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      7219..7228
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7248..7459)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        7460..7839
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag cttgcctata taagcagagc tcgtttagtg aaccgtcaga
      301 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      361 gcctaccaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
      421 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
      481 ccagtcacta tggcgacaga agaagtatag ggataacagg gtaattgttg taagcgcgct
      541 atgatggagg ctatgccact agaatctgcg ttcgctacct taggaccgtt atagttagaa
      601 ggaaagctcc atcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
      661 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
      721 ttcttgtaca aagtggtata cccatacgat gttccagatt acgcttagta atgagaaata
      781 ctcagaagat gtcactcacc atagagagac cattaatggg tctcctaggc cactgagcaa
      841 gaactatgaa tgagatctcc cctctccctc ccccccccct aacgttactg gccgaagccg
      901 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
      961 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     1021 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     1081 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     1141 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     1201 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     1261 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     1321 tgatctgggg cctcggtaca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     1381 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
     1441 caaccatgag tgaactcata aaggaaaata tgcacatgaa actttatatg gaaggtacag
     1501 tcgacaatca ccatttcaaa tgtacctcag aaggagaggg caagccatac gaaggaaccc
     1561 aaaccatgcg aataaaggta gtggaggggg gtcctctccc ctttgcattt gatatcctcg
     1621 caacgtcttt cctgtatggc tcaaaaacct tcataaatca tacgcagggc ataccagact
     1681 tcttcaaaca gtcctttccg gagggcttca cttgggaaag agtcacgact tatgaagatg
     1741 gcggcgtcct gacagcaact caggacacat ctctacagga tggctgcctc atctataatg
     1801 tgaagattcg gggagttaac ttcactagca acggacctgt tatgcagaag aaaacgcttg
     1861 gttgggaagc atttacggaa actctttatc ctgctgatgg tgggcttgaa ggtcgcaacg
     1921 acatggcact caaactggtt ggagggagcc accttatcgc caatgcaaag acgacttatc
     1981 gctctaaaaa accagctaaa aacctgaaga tgcccggtgt gtactatgta gactaccgcc
     2041 tggaacgaat aaaagaagcc aataacgaga cttatgtgga gcagcatgaa gtggcggtgg
     2101 cacggtactg tgatctgccg agcaaacttg ggcataaact gaattaagcg gccgctttct
     2161 ccgcgatcca gacatgataa gatacattga tgagtttgga caaaccaaaa ctagaatgca
     2221 gtgaaaaaaa tgccttattt gtgaaatttg tgatgctatt gccttatttg taaccattat
     2281 aagctgcaat aaacaagtta tctataacaa gaaaatatat atataataag ttatcacgta
     2341 agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa
     2401 agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta
     2461 ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca
     2521 cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa
     2581 ttttacgcag actatctttc tagggttaag aattcactgg ccgtcgtttt acaacgtcgt
     2641 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     2701 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     2761 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     2821 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     2881 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     2941 agacaagctg tgaccatgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     3001 cgaaacacgc gagttcgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     3061 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     3121 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3181 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3241 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3301 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3361 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     3421 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     3481 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     3541 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     3601 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     3661 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     3721 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     3781 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     3841 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     3901 gctgataaat ctggagccgg tgagcgtggt tcacgcggta tcattgcagc actggggcca
     3961 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4021 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4081 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4141 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4201 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4261 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4321 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4381 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4441 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4501 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4561 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4621 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4681 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4741 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4801 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4861 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4921 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4981 gagcgcagcg agtcagtgag cgaggaagcg gatgagcgcc caatacgcaa accgcctctc
     5041 accgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     5101 ggcagtgagc gcaacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     5161 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     5221 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     5281 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagg
     5341 tcgacttaac cctagaaaga taatcatatt gtgacgtacg ttaaagataa tcatgcgtaa
     5401 aattgacgca tgtgttttat cggtctgtat atcgaggttt atttattaat ttgaatagat
     5461 attaagtttt attatattta cacttacata ctaataataa attcaacaaa caatttattt
     5521 atgtttattt atttattaaa aaaaaacaaa aactcaaaat ttcttctata aagtaacaaa
     5581 acttttagca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     5641 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     5701 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     5761 tggctgatta tgatcctctg gagattcatg cgccaggttt tctggtcatg caccacgttc
     5821 gtgggccctc aggaacctcc acgtcagcgg taactgtgaa gcccagtcgc tcgtaaaaag
     5881 gaagattccg gggagcactg gtttcgagga acgccggcac gccagcccgt tccgcggcct
     5941 caacaccagg aagaacgaca gcgcttccca aacctttacc ttggtgatct gggctgacac
     6001 cgacagtggc aagaaaccac gcaggctcct ttggtctatg aggagccagc aagccctcca
     6061 tttgctgttg tgccgccaac ctactccctg acagctcggc catccgcgga cctatttccg
     6121 cgaacactgc gccggcttcg acgctttccg gagtagtcca gaccgcaact gcggcaccat
     6181 cgtccgcaac ccaaacttta ccaatgtcca gacccacgcg cgtaagaaac agctcttgca
     6241 actcagttac cctctctatg tgtctgtccg gatctacggt atggcgggtc gctggataat
     6301 ctgcaaatgc tgcggcaagt gttcggacag cccgtggtac atcgtcacgg gtagcgagcc
     6361 taacagtggg cttatattcc gtcatggggc cggggttctc ctccacgtcg ccggcctgct
     6421 tcagcaggct gaagttggtg gcgccgctgc ccccggggag catgtcaagg tcaaaatcgt
     6481 caagagcgtc agcaggcagc atatcaaggt caaagtcgtc aagggcatcg gctgggagca
     6541 tgtctaagtc aaaatcgtca agggcgtcgg tcggcccgcc gctttcgcac tttagctgtt
     6601 tctccaggcc acatatgatt agttccaggc cgaaaaggaa ggcaggttcg gctccctgcc
     6661 ggtcgaacag ctcaattgct tgtttcagaa gtgggggcat agaatcggtg gtaggtgtct
     6721 ctctttcctc ttttgctact tgatgctcct gttcctccaa tacgcagccc agtgtaaagt
     6781 ggcccacggc ggacagagcg tacagtgcgt tctccaggga gaagccttgc tgacacagga
     6841 acgcgagctg attttccagg gtttcgtact gtttctctgt tgggcgggtg ccgagatgca
     6901 ctttagcccc gtcacgatgt gagaggagag cacagcggta tgacttggcg ttgttccgca
     6961 gaaagtcttg ccatgactcg ccttccaggg ggcaggagtg ggtatgatgc ctgtccagca
     7021 tctcgattgg cagggcatcg agcagggccc gcttgttctt cacgtgccag tacagggtag
     7081 gctgctcaac tcccagcttt tgagcgagtt tccttgtcgt caggccttcg ataccgactc
     7141 cattgagtaa ttccagagca gagtttatga ctttgctctt gtccagtcta gacatcttat
     7201 cgtcatcgtc tttgtaatcc atggtggcgg atcccgcgtc acgacacctg tgttctggcg
     7261 gcaaacccgt tgcgaaaaag aacgttcacg gcgactactg cacttatata cggttctccc
     7321 ccaccctcgg gaaaaaggcg gagccagtac acgacatcac tttcccagtt taccccgcgc
     7381 caccttctct aggcaccggt tcaattgccg acccctcccc ccaacttctc gggcactgtg
     7441 ggccatgtgc gctctgcccc atggtaatag ccatgactaa tacgtagatg tactgccaag
     7501 taggaaagtg ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     7561 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
     7621 tttaccgtaa atactccacc cattgacgtc aatggaaagt gcctattggc gttactatgg
     7681 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
     7741 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
     7801 taattgatta ctattaataa ctagtcaata atcaatgtca taagctctaa tcgcataatc
     7861 gagctaagag cgattaatat gcttagctag cttagctaag ctagatttct ctattctccc
     7921 acaatatata gcagctatta gcttagcagt ccgaatacga tcgtccacga tccataagcg
     7981 taacacattt gagtagcgac tgacgggcac cggagccact cgagtggaat t
//
