LOCUS       sequence-34512-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral transfer plasmid for Tet-regulated shRNA expression with 
            a minimal CMV promoter. Contains DsRed2 and Venus fluorescent 
            reporters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Zuber J, McJunkin K, Fellmann C, Dow LE, Taylor MJ, Hannon GJ, Lowe 
            SW
  TITLE     Toolkit for evaluating genes required for proliferation and survival
            using tetracycline-regulated RNAi.
  JOURNAL   Nat Biotechnol. 2011 Jan . 29(1):79-83.
  PUBMED    21131983
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jan . 29(1):79-83."
COMMENT     Sequence Label: TRMPV-Neo
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             219..644
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(747..769)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
       61 ttcttgacga gttcttctga tgtacgatgc atcgataccg tcgagatatc agtggtccag
      121 gctctagttt tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata
      181 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
      241 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga
      301 atagagaagt tcagatcaag gtcaggaaca gatggaacag ggtcgaccct agagaaccat
      361 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
      421 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
      481 ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg
      541 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
      601 tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg
      661 atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggctgcctc
      721 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
      781 gcttgtctgt aagcggatgc cgggagcaga caagc
//
