LOCUS       sequence-34513-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral transfer plasmid for Tet-regulated shRNA expression with 
            a minimal CMV promoter. Contains DsRed2 and Venus fluorescent 
            reporters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Zuber J, McJunkin K, Fellmann C, Dow LE, Taylor MJ, Hannon GJ, Lowe 
            SW
  TITLE     Toolkit for evaluating genes required for proliferation and survival
            using tetracycline-regulated RNAi.
  JOURNAL   Nat Biotechnol. 2011 Jan . 29(1):79-83.
  PUBMED    21131983
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jan . 29(1):79-83."
COMMENT     Sequence Label: TRMPV-Neo
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           103..224
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           252..266
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           294..420
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(478..501)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gcgcaccgag ggccgccacc acctgttcct gtagcggccg cgactctaga atttactagt
       61 catatgataa gatctctcga ctagggataa cagggtaatt gtttgaatga ggcttcagta
      121 ctttacagaa tcgttgcctg cacatcttgg aaacacttgc tgggattact tcttcaggtt
      181 aacccaacag aaggctcgag aaggtatatt gctgttgaca gtgagcgcag gaattataat
      241 gcttatctat agtgaagcca cagatgtata gataagcatt ataattccta tgcctactgc
      301 ctcggaattc aaggggctac tttaggagca attatcttgt ttactaaaac tgaatacctt
      361 gctatctctt tgatacattt ttacaaagct gaattaaaat ggtataaatt aaatcacttt
      421 tttcaattct agcaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
      481 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
      541 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
      601 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
      661 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
      721 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
      781 tgggctca
//
