LOCUS       sequence-345154-        9237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant GST-SLFN2-K242A, K249A, Q301A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Yue T, Zhan X, Zhang D, Jain R, Wang KW, Choi JH, Misawa T, Su L, 
            Quan J, Hildebrand S, Xu D, Li X, Turer E, Sun L, Moresco EMY, 
            Beutler B
  TITLE     SLFN2 protection of tRNAs from stress-induced cleavage is essential 
            for T cell-mediated immunity.
  JOURNAL   Science. 2021 May 14;372(6543). pii: 372/6543/eaba4220. doi: 
            10.1126/science.aba4220.
  PUBMED    33986151
REFERENCE   2  (bases 1 to 9237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2021-05-14"; volume: "372(6543). pii: 372/6543/eaba4220. 
            doi"; pages: " 10.1126/science.aba4220"
COMMENT     Sequence Label: pEZT-GST-SLFN2-T2
FEATURES             Location/Qualifiers
     source          1..9237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     165..184
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     424..445
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(512..621)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     enhancer        806..1185
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1186..1389
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1340..1360
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1386..1410
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1525..1657
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1640..1659
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1702..1720
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1746..2399
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     2357..2379
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             2406..2429
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     misc_feature    3597..4185
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3650..3670)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4068..4079)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4327..4461
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4364..4383)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4418..4437
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4490..4655
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4839..5293
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4926..4945)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5135..5156
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5319..5423
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5424..5492,5493..6284)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5642..5661)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6455..7043
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6944..6963
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7197..7214
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  7348..7572
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(7639..8172)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(8342..8347,8348..8364,8365..8370))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    8878..8926
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     8951..8970
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(join(9011..9237,1..484,485..487))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(9053..9074)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
       61 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
      121 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
      181 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
      241 agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg
      301 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
      361 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
      421 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
      481 tgctcaccgg gtgatcaagt cttcgtcgag tgattgtaaa taaaatgtaa tttacagtat
      541 agtattttaa ttaatataca aatgatttga taataattct tatttaacta taatatattg
      601 tgttgggttg aattaaaggt ccgtatactc cggaatatta ataggatccc ggtccgagcg
      661 cgcggaattc aatattggcc attagccata ttattcattg gttatatagc ataaatcaat
      721 attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc
      781 tcatgtccaa tatgaccgcc atgttggcat tgattattga ctagttatta atagtaatca
      841 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      901 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      961 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     1021 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac
     1081 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt
     1141 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     1201 cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     1261 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     1321 aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     1381 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
     1441 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcaga
     1501 agttggtcgt gaggcactgg gcaggtaagt atcaaggtta caagacaggt ttaaggagac
     1561 caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata ggcacctatt
     1621 ggtcttactg acatccactt tgcctttctc tccacaggtg tccactccca gttcaattac
     1681 agctcttaag gctagagtac ttaatacgac tcactatagg ctagcctcga ggcggccgcg
     1741 ccaccatgag ccctatacta ggttattgga aaattaaggg ccttgtgcaa cccactcgac
     1801 ttcttttgga atatcttgaa gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg
     1861 ataaatggcg aaacaaaaag tttgaattgg gtttggagtt tcccaatctt ccttattata
     1921 ttgatggtga tgttaaatta acacagtcta tggccatcat acgttatata gctgacaagc
     1981 acaacatgtt gggtggttgt ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg
     2041 ttttggatat tagatacggt gtttcgagaa ttgcatatag taaagacttt gaaactctca
     2101 aagttgattt tcttagcaag ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc
     2161 ataaaacata tttaaatggt gatcatgtaa cccatcctga cttcatgttg tatgacgctc
     2221 ttgatgttgt tttatacatg gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt
     2281 ttaaaaaacg tattgaagct atcccacaaa ttgataagta cttgaaatcc agcaagtata
     2341 tagcatggcc tttgcagggc tggcaagcca cgtttggtgg tggcgaccat cctccaaaat
     2401 cggatctgga agttctgttc caggggcccc tgggatcccc ggaattcatg ggtactagac
     2461 ttgaggcaac tgagcaaagc aaccacagaa ctcagaggaa tgacattcag ctggaaaatg
     2521 caaaggctgc tgggaaaatg ggcatcagtg ttgatctgga agccaaatat gctaagctgg
     2581 gtctaaatct cggagcaatc acttttggag agaaggatag gaagaaaatg aagaattctc
     2641 acctcagaaa acaggagaat gcaaacatct ctctagctgt atgtgctctc ctgaattcgg
     2701 gaggtggagc aatcaaggtt aaaattgaaa atgaaaatta tagtctcact agggatggcc
     2761 tgggactaga tttggaagcc tctctttgta aatgtctgcc ctttgtccag tggcacctgg
     2821 acttcacgga gagcgaaggc tacatttata tctacgtgaa atcgtggagc caagaaatct
     2881 ttgggctgcc tattggcacc ctaagaacca atttgtatgt aaggagcatg tcatcttctg
     2941 tacaagtgag cgccgctgct gccctggaat ttctccagga cctggaggaa actggaggga
     3001 gaccctgtgt cagaccagag ttgcctgcaa gcatagcctt ccctgaagtg gaaggagaat
     3061 ggcacctgga ggatttggct gctgcattgt ttaacaggac agaatttcag tacgaggaaa
     3121 ctttcccctt taccagatcc agatatgttg aagttacatt gctttcagcg gcacgcctgc
     3181 gaaaacgcat cgcagagctc ctccctcaaa ctgtttctgc ttttgcaaac acggatgggg
     3241 gatttttgtt cattggtttg gatggcaaaa cccagcaaat tattggtttt gaagcagaga
     3301 agagcgatct cgtgcttcta gagagtgaaa tagaaaagca catccgggcg ctgcctgtca
     3361 ctcacttctg tgaggagaag gagaagatca agtacacgtg caaattcatc gaagtgcaca
     3421 aatccggagc tgtgtgtgca tatgtgtgtg cgctcagagt ggagagattc tgctgtgcag
     3481 tattcgctgc agagcccgag tcctggcatg tggaaggcgg ctgtgtgaag aggtttacca
     3541 cagaggaatg ggtgaaactc cagatgaatg ccccatcagg ttgaggtacc aagcttaatc
     3601 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     3661 ttacgctatg tgaatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     3721 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     3781 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     3841 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     3901 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     3961 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     4021 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     4081 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     4141 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgca gtctgacaag
     4201 cttgtcgaga agtactagag gatcataatc agccatacca catttgtaga ggttttactt
     4261 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     4321 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     4381 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     4441 gtatcttatc atgtctggat ctgatcactg cttgagccta ggagatccga accagataag
     4501 tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt acgacgctac
     4561 acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac tccatttcca
     4621 cccctcccag ttcccaacta ttttgtccgc ccacagcggg gcatttttct tcctgttatg
     4681 tttttaatca aacatcctgc caactccatg tgacaaaccg tcatcttcgg ctactttttc
     4741 tctgtcacag aatgaaaatt tttctgtcat ctcttcgtta ttaatgtttg taattgactg
     4801 aatatcaacg cttatttgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     4861 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4921 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4981 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5041 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5101 tcgccctttg acgttggagt ccacgttctt aatagtggac tcttgttcca aactggaaca
     5161 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5221 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5281 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5341 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5401 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5461 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5521 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5581 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5641 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5701 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5761 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5821 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5881 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5941 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6001 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6061 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6121 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6181 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6241 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6301 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6361 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6421 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6481 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6541 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6601 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6661 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6721 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6781 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6841 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6901 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6961 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7021 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7081 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7141 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7201 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     7261 gcggtatttc acaccgcata gaccagccgc gtaacctggc aaaatcggtt acggttgagt
     7321 aataaatgga tgccctgcgt aagcgggtgt gggcggacaa taaagtctta aactgaacaa
     7381 aatagatcta aactatgaca ataaagtctt aaactagaca gaatagttgt aaactgaaat
     7441 cagtccagtt atgctgtgaa aaagcatact ggacttttgt tatggctaaa gcaaactctt
     7501 cattttctga agtgcaaatt gcccgtcgta ttaaagaggg gcgtggccaa gggcatggta
     7561 aagactatat tcgcggcgtt gtgacaattt accgaacaac tccgcggccg ggaagccgat
     7621 ctcggcttga acgaattgtt aggtggcggt acttgggtcg atatcaaagt gcatcacttc
     7681 ttcccgtatg cccaactttg tatagagagc cactgcggga tcgtcaccgt aatctgcttg
     7741 cacgtagatc acataagcac caagcgcgtt ggcctcatgc ttgaggagat tgatgagcgc
     7801 ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg agatcataga tatagatctc
     7861 actacgcggc tgctcaaact tgggcagaac gtaagccgcg agagcgccaa caaccgcttc
     7921 ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg agcaagttcc cgaggtaatc
     7981 ggagtccggc tgatgttggg agtaggtggc tacgtctccg aactcacgac cgaaaagatc
     8041 aagagcagcc cgcatggatt tgacttggtc agggccgagc ctacatgtgc gaatgatgcc
     8101 catacttgag ccacctaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct
     8161 gctgcgtaac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg
     8221 catagactgt acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc
     8281 accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta
     8341 cagtttacga accgaacagg cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg
     8401 tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc
     8461 gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt
     8521 ctacggcaag gtgctgtgca cggatctgcc cttgcttcag gagatcggta gacctcggcc
     8581 gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct
     8641 ggaaggcgag catcgtttgt tcgcccagga ctctagctat agttctagtg gttggctacg
     8701 tacccgtagt ggctatggca gggcttgccg ccccgacgtt ggctgcgagc cctgggcctt
     8761 cacccgaact tgggggttgg ggtggggaaa aggaagaaac gcgggcgtat tggtcccaat
     8821 ggggtctcgg tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa
     8881 caaacgaccc aacacccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt
     8941 ccttccggta ttgtctcctt ccgtgtttca gttagcctcc cccatctccc ggttccgcat
     9001 gctatgcatc cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     9061 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactgggtgc
     9121 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     9181 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttca
//
