LOCUS       sequence-345155-        9237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant GST-SLFN2-R67A, K68A, N71A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Yue T, Zhan X, Zhang D, Jain R, Wang KW, Choi JH, Misawa T, Su L, 
            Quan J, Hildebrand S, Xu D, Li X, Turer E, Sun L, Moresco EMY, 
            Beutler B
  TITLE     SLFN2 protection of tRNAs from stress-induced cleavage is essential 
            for T cell-mediated immunity.
  JOURNAL   Science. 2021 May 14;372(6543). pii: 372/6543/eaba4220. doi: 
            10.1126/science.aba4220.
  PUBMED    33986151
REFERENCE   2  (bases 1 to 9237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2021-05-14"; volume: "372(6543). pii: 372/6543/eaba4220. 
            doi"; pages: " 10.1126/science.aba4220"
COMMENT     Sequence Label: pEZT-GST-SLFN2-T1
FEATURES             Location/Qualifiers
     source          1..9237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(112..645)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(815..820,821..837,838..843))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    1351..1399
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     1424..1443
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(join(1484..2194,2195..2197))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1526..1547)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1875..1894
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2134..2155
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(2222..2331)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     enhancer        2516..2895
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2896..3099
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3050..3070
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3096..3120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          3235..3367
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3350..3369
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        3412..3430
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3456..4109
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     4067..4089
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             4116..4139
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     misc_feature    5307..5895
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5360..5380)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5778..5789)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6037..6171
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6074..6093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6128..6147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  6200..6365
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      6549..7003
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6636..6655)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6845..6866
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7029..7133
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7134..7202,7203..7994)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7352..7371)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8165..8753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8654..8673
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8907..8924
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  join(9058..9237,1..45)
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
ORIGIN
        1 gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa
       61 tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc
      121 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
      181 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
      241 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc
      301 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
      361 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
      421 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
      481 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
      541 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
      601 agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcaaac atcgacccac
      661 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata
      721 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
      781 ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt
      841 caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag
      901 cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac
      961 gcatcgtcag gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct
     1021 gcccttgctt caggagatcg gtagacctcg gccgtcgcgg cgcttgccgg tggtgctgac
     1081 cccggatgaa gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca
     1141 ggactctagc tatagttcta gtggttggct acgtacccgt agtggctatg gcagggcttg
     1201 ccgccccgac gttggctgcg agccctgggc cttcacccga acttgggggt tggggtgggg
     1261 aaaaggaaga aacgcgggcg tattggtccc aatggggtct cggtggggta tcgacagagt
     1321 gccagccctg ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta
     1381 ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt
     1441 tcagttagcc tcccccatct cccggttccg catgctatgc atccttgtac agctcgtcca
     1501 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
     1561 cgttggggtc tttgctcagc ttggactggg tgctcaggta gtggttgtcg ggcagcagca
     1621 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
     1681 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
     1741 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
     1801 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
     1861 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
     1921 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
     1981 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
     2041 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
     2101 cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
     2161 gcaccacccc ggtgaacagc tcctcgccct tgctcaccgg gtgatcaagt cttcgtcgag
     2221 tgattgtaaa taaaatgtaa tttacagtat agtattttaa ttaatataca aatgatttga
     2281 taataattct tatttaacta taatatattg tgttgggttg aattaaaggt ccgtatactc
     2341 cggaatatta ataggatccc ggtccgagcg cgcggaattc aatattggcc attagccata
     2401 ttattcattg gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat
     2461 ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgttggcat
     2521 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     2581 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     2641 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     2701 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     2761 tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     2821 tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc
     2881 atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg atagcggttt
     2941 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     3001 caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc
     3061 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
     3121 actagaagct ttattgcggt agtttatcac agttaaattg ctaacgcagt cagtgcttct
     3181 gacacaacag tctcgaactt aagctgcaga agttggtcgt gaggcactgg gcaggtaagt
     3241 atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc gagacagaga
     3301 agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt tgcctttctc
     3361 tccacaggtg tccactccca gttcaattac agctcttaag gctagagtac ttaatacgac
     3421 tcactatagg ctagcctcga ggcggccgcg ccaccatgag ccctatacta ggttattgga
     3481 aaattaaggg ccttgtgcaa cccactcgac ttcttttgga atatcttgaa gaaaaatatg
     3541 aagagcattt gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag tttgaattgg
     3601 gtttggagtt tcccaatctt ccttattata ttgatggtga tgttaaatta acacagtcta
     3661 tggccatcat acgttatata gctgacaagc acaacatgtt gggtggttgt ccaaaagagc
     3721 gtgcagagat ttcaatgctt gaaggagcgg ttttggatat tagatacggt gtttcgagaa
     3781 ttgcatatag taaagacttt gaaactctca aagttgattt tcttagcaag ctacctgaaa
     3841 tgctgaaaat gttcgaagat cgtttatgtc ataaaacata tttaaatggt gatcatgtaa
     3901 cccatcctga cttcatgttg tatgacgctc ttgatgttgt tttatacatg gacccaatgt
     3961 gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct atcccacaaa
     4021 ttgataagta cttgaaatcc agcaagtata tagcatggcc tttgcagggc tggcaagcca
     4081 cgtttggtgg tggcgaccat cctccaaaat cggatctgga agttctgttc caggggcccc
     4141 tgggatcccc ggaattcatg ggtactagac ttgaggcaac tgagcaaagc aaccacagaa
     4201 ctcagaggaa tgacattcag ctggaaaatg caaaggctgc tgggaaaatg ggcatcagtg
     4261 ttgatctgga agccaaatat gctaagctgg gtctaaatct cggagcaatc acttttggag
     4321 agaaggatag gaagaaaatg aagaattctc acctcgcagc acaggaggct gcaaacatct
     4381 ctctagctgt atgtgctctc ctgaattcgg gaggtggagc aatcaaggtt aaaattgaaa
     4441 atgaaaatta tagtctcact agggatggcc tgggactaga tttggaagcc tctctttgta
     4501 aatgtctgcc ctttgtccag tggcacctgg acttcacgga gagcgaaggc tacatttata
     4561 tctacgtgaa atcgtggagc caagaaatct ttgggctgcc tattggcacc ctaagaacca
     4621 atttgtatgt aaggagcatg tcatcttctg tacaagtgag cgccgctgct gccctggaat
     4681 ttctccagga cctggaggaa actggaggga gaccctgtgt cagaccagag ttgcctgcaa
     4741 gcatagcctt ccctgaagtg gaaggagaat ggcacctgga ggatttggct gctgcattgt
     4801 ttaacaggac agaatttcag tacgaggaaa ctttcccctt taccagatcc agatatgttg
     4861 aagttacatt gctttcagcg aaacgcctgc gaaaacgcat caaagagctc ctccctcaaa
     4921 ctgtttctgc ttttgcaaac acggatgggg gatttttgtt cattggtttg gatggcaaaa
     4981 cccagcaaat tattggtttt gaagcagaga agagcgatct cgtgcttcta gagagtgaaa
     5041 tagaaaagca catccggcag ctgcctgtca ctcacttctg tgaggagaag gagaagatca
     5101 agtacacgtg caaattcatc gaagtgcaca aatccggagc tgtgtgtgca tatgtgtgtg
     5161 cgctcagagt ggagagattc tgctgtgcag tattcgctgc agagcccgag tcctggcatg
     5221 tggaaggcgg ctgtgtgaag aggtttacca cagaggaatg ggtgaaactc cagatgaatg
     5281 ccccatcagg ttgaggtacc aagcttaatc aacctctgga ttacaaaatt tgtgaaagat
     5341 tgactggtat tcttaactat gttgctcctt ttacgctatg tgaatacgct gctttaatgc
     5401 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     5461 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     5521 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     5581 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5641 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     5701 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     5761 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     5821 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     5881 gggccgcctc cccgcctgca gtctgacaag cttgtcgaga agtactagag gatcataatc
     5941 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     6001 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     6061 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6121 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg
     6181 cttgagccta ggagatccga accagataag tgaaatctag ttccaaacta ttttgtcatt
     6241 tttaattttc gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc
     6301 cctaaataat ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc
     6361 ccacagcggg gcatttttct tcctgttatg tttttaatca aacatcctgc caactccatg
     6421 tgacaaaccg tcatcttcgg ctactttttc tctgtcacag aatgaaaatt tttctgtcat
     6481 ctcttcgtta ttaatgtttg taattgactg aatatcaacg cttatttgca gcctgaatgg
     6541 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6601 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6661 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6721 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6781 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6841 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6901 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6961 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     7021 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7081 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7141 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7201 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7261 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7321 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7381 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7441 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7501 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7561 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7621 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7681 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7741 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7801 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7861 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7921 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7981 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8041 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8101 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8161 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8221 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8281 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8341 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8401 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8461 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8521 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8581 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8641 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8701 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8761 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8821 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8881 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8941 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata gaccagccgc
     9001 gtaacctggc aaaatcggtt acggttgagt aataaatgga tgccctgcgt aagcgggtgt
     9061 gggcggacaa taaagtctta aactgaacaa aatagatcta aactatgaca ataaagtctt
     9121 aaactagaca gaatagttgt aaactgaaat cagtccagtt atgctgtgaa aaagcatact
     9181 ggacttttgt tatggctaaa gcaaactctt cattttctga agtgcaaatt gcccgtc
//
