LOCUS       sequence-34518-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral transfer plasmid for Tet-regulated shRNA expression with 
            a minimal CMV promoter. Contains DsRed2 and Venus fluorescent 
            reporters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Zuber J, McJunkin K, Fellmann C, Dow LE, Taylor MJ, Hannon GJ, Lowe 
            SW
  TITLE     Toolkit for evaluating genes required for proliferation and survival
            using tetracycline-regulated RNAi.
  JOURNAL   Nat Biotechnol. 2011 Jan . 29(1):79-83.
  PUBMED    21131983
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jan . 29(1):79-83."
COMMENT     Sequence Label: TRMPV-Hygro
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
       61 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
      121 acacgatgat aagcttaccg gtgccaccat gaaaaagcct gaactcaccg cgacgtctgt
      181 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
      241 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
      301 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
      361 gctcccgatt ccggaagtgc ttgacattgg ggaatttagc gagagcctga cctattgcat
      421 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
      481 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
      541 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
      601 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
      661 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
      721 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
      781 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg ag
//
