LOCUS       sequence-34519-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral transfer plasmid for Tet-regulated shRNA expression with 
            a minimal CMV promoter. Contains DsRed2 and Venus fluorescent 
            reporters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Zuber J, McJunkin K, Fellmann C, Dow LE, Taylor MJ, Hannon GJ, Lowe 
            SW
  TITLE     Toolkit for evaluating genes required for proliferation and survival
            using tetracycline-regulated RNAi.
  JOURNAL   Nat Biotechnol. 2011 Jan . 29(1):79-83.
  PUBMED    21131983
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jan . 29(1):79-83."
COMMENT     Sequence Label: TRMPV-Hygro
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           106..227
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           255..269
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           297..423
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(481..504)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cgagcgcacc gagggccgcc accacctgtt cctgtagcgg ccgcgactct agaatttact
       61 agtcatatga taagatctct cgactaggga taacagggta attgtttgaa tgaggcttca
      121 gtactttaca gaatcgttgc ctgcacatct tggaaacact tgctgggatt acttcttcag
      181 gttaacccaa cagaaggctc gagaaggtat attgctgttg acagtgagcg cccgcctgaa
      241 gtctctgatt aatagtgaag ccacagatgt attaatcaga gacttcaggc ggttgcctac
      301 tgcctcggaa ttcaaggggc tactttagga gcaattatct tgtttactaa aactgaatac
      361 cttgctatct ctttgataca tttttacaaa gctgaattaa aatggtataa attaaatcac
      421 ttttttcaat tctagcaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
      481 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
      541 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
      601 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
      661 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
      721 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
      781 ttctgggctc a
//
