LOCUS       sequence-345192-        2467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic 5xUAS GAL4 driver response element in enhancer grammar, 
            requires minimal promoter for assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: p5xUAS
FEATURES             Location/Qualifiers
     source          1..2467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(286..741)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(423..444)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     635..654
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(855..1670)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(855..1670)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1578..1597
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1671..1773)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      join(1888..2467,1..9)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2377..2396
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
       61 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtgt cagaattcgt
      121 ctcatgacag cgcggaggac agtactccgc tcctgacagc gcggaggaca gtactccgct
      181 cctgacagcg cggaggacag tactccgctc ctgacagcgc ggaggacagt actccgctcc
      241 tgacagcgcg gaggacagta ctccgctcct gagacgaatt cgttaaaatt gtaagcgtta
      301 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
      361 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
      421 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
      481 aaaccgtcta tcagggcgct ggcccactac gtgaaccatc accctaatca agttttttgg
      541 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
      601 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
      661 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
      721 atgcgccgct acagggcgcg taacatcatg tttgacagct tatcatcgaa tttctgccat
      781 tcatccgctt attatcactt attcaggcgt agcaaccagg cgtttaaggg caccaataac
      841 tgccttaaaa aaaatcagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
      901 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
      961 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
     1021 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
     1081 ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact
     1141 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
     1201 ttcattcgtg attgcgcctg agcgagtcga aatacgcgat cgctgttaaa aggacaatta
     1261 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
     1321 cctgaatcag gatattcttc taatacctgg aatgctgttt tccctgggat cgcagtggtg
     1381 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
     1441 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
     1501 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcggta gattgtcgca
     1561 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
     1621 gaatttaatc gcggccttga gcaagacgtt tcccgttgaa tatggctcat tttagcttcc
     1681 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     1741 tggtgaaagt tggaacctct tacgtgccga tcatcacaaa aggatctagg tgaagatcct
     1801 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1861 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1921 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1981 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2041 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2101 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2161 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2221 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2281 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2341 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2401 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2461 gcggagc
//
