LOCUS       sequence-345194-        3039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial rrnB gene T1 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: prrnBT1_STD
FEATURES             Location/Qualifiers
     source          1..3039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(22..94)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(148..167)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(149..167)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(174..191)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(174..190)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      332..359
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      385..840
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(472..491)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     682..703
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        918..1022
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1023..1091,1092..1883)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1241..1260)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2054..2642
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2543..2562
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2796..2813
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2828..2899)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2970..2986
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2970..2986
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3002..3020
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3002..3019
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ggcgatgagt ggtctcgagc ggagagcgtt caaccgacaa acaacagata aaacgaaagg
       61 cccagtcttt cgactgagcc tttcgtttta tttgatgcct gaagccgaga ccttgagatc
      121 aatatctaag gtcctaagcg gaattcaccc tatagtgagt cgtattacaa ttcactggcc
      181 gtcgttttac attgagaaga gaaaagaaaa ccgccgatcc tgtccaccgc attactgcaa
      241 ggtagtggac aagaccggcg gtcttaagtt ttttggctga aagggtggcg ggcaggacgc
      301 ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt
      361 gcgtttctac aatgttacga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      421 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      481 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      541 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
      601 agggtgatgg ttcacgtagt gggccagcgc cctgatagac ggtttttcgc cctttgacgt
      661 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
      721 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
      781 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
      841 cctgatgcgg tattttctac ttacgcatct gtgcggtatt tcacaccgca tcaggtggca
      901 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      961 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1021 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1081 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1141 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1201 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1261 cccgtattga cgccgggcaa gagcaactag gtcgccgcat acactattct cagaatgact
     1321 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1381 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1441 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1501 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1561 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1621 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1681 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1741 cgcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1801 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1861 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     1921 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1981 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2041 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2101 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2161 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2221 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2281 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2341 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2401 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2461 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2521 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2581 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2641 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2701 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2761 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2821 aaggtcagag agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact
     2881 gagcctttcg ttttatttga tgcctgctcg gtaccaaatt ccagaaaaga ggcctcccga
     2941 aaggggggcc ttttttcgtt ttggtccgac aggaaacagc tatgaccaac ggctctatgg
     3001 tatttaggtg acactataga acgaattcac tagtgattg
//
