LOCUS       sequence-345196-        3040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Standard grammar bacterial CP6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pCP6-STD
FEATURES             Location/Qualifiers
     source          1..3040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(97..116)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(98..116)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(123..140)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(123..139)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      334..789
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(421..440)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     631..652
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        867..971
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(972..1040,1041..1832)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1190..1209)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2003..2591
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2492..2511
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2745..2762
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2777..2848)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2919..2935
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2919..2935
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2951..2969
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2951..2968
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gatatttatg atataataac tgagtaagct taacataagg aggaaaacaa aatgcgagac
       61 cttgagatca atatctaagg tcctaagcgg aattcaccct atagtgagtc gtattacaat
      121 tcactggccg tcgttttaca ttgagaagag aaaagaaaac cgccgatcct gtccaccgca
      181 ttactgcaag gtagtggaca agaccggcgg tcttaagttt tttggctgaa agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca atgttacgaa tggacgcgcc ctgtagcggc gcattaagcg
      361 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      421 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      481 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      541 aacttgatta gggtgatggt tcacgtagtg ggccagcgcc ctgatagacg gtttttcgcc
      601 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
      661 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
      721 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
      781 ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
      841 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      901 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      961 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1021 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1081 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1141 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1201 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1261 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1321 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1381 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1441 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1501 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1561 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1621 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1681 agcgtgggtc gcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1741 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1801 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1861 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1921 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1981 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2041 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2101 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     2161 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2221 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2281 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2341 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2401 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2461 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2521 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2581 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2641 ttgctcacat gttctttcct gcgttatccc atgattctgt ggataaccgt attaccgcct
     2701 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2761 aggaagcgga aggtcagaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     2821 ctttcgactg agcctttcgt tttatttgat gcctgctcgg taccaaattc cagaaaagag
     2881 gcctcccgaa aggggggcct tttttcgttt tggtccgaca ggaaacagct atgaccaacg
     2941 gctctatggt atttaggtga cactatagaa cgaattcact agtgattggg cgatgagtgg
     3001 tctcgggagc gaatccatgt gggagtttat tcttgacaca
//
