LOCUS       sequence-345197-        2423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3xP3 eye driver enhancer element, requires promoter element for 
            function.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: p3xP3
FEATURES             Location/Qualifiers
     source          1..2423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     12..29
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13..29
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(218..236)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(219..236)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(252..268)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(252..268)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(383..971)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(463..482)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1086..1188
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1189..1848
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1255..1274)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1961..2416
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2048..2067)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2258..2279
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtagaaacgc atgtaaaacg acggccagtg aattgtaata cgactcacta tagggtgaat
       61 tccgcttagg accttagata ttgatctcaa ggtctcgggg agagggttcg aaatcgataa
      121 gcttggatcc taattgaatt agctctaatt gaattagtct ctaattgaat tagatccctc
      181 ccgagaccac tcatcgccca atcactagtg aattcgttct atagtgtcac ctaaatacca
      241 tagagccgtt ggtcatagct gtttcctgtc caggcatcat gacacggtta tccacagaat
      301 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      361 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      421 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      481 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      541 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      601 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      661 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      721 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      781 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
      841 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
      901 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
      961 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1021 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1081 tgtgatgatc ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
     1141 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     1201 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     1261 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     1321 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     1381 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
     1441 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     1501 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     1561 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     1621 gtgtcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     1681 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     1741 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
     1801 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc
     1861 agttattggt gcccttaaac gcctggttgc tacgcctgaa taagtgataa taagcggatg
     1921 aatggcagaa attcgatgat aagctgtcaa acatgatgtt acgcgccctg tagcggcgca
     1981 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     2041 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2101 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2161 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc cagcgccctg atagacggtt
     2221 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     2281 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     2341 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     2401 ttaacgctta caattttaac att
//
