LOCUS       sequence-345199-       10334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Vp64-dCas9 activation system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Penev A, Bazley A, Shen M, Boeke JD, Savage SA, Sfeir A
  TITLE     Alternative splicing is a developmental switch for hTERT expression.
  JOURNAL   Mol Cell. 2021 Apr 6. pii: S1097-2765(21)00228-8. doi: 
            10.1016/j.molcel.2021.03.033.
  PUBMED    33852895
REFERENCE   2  (bases 1 to 10334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Apr 6. pii: S1097-2765(21)00228-8. doi: 
            10.1016/j.molcel.2021.03.033."
COMMENT     Sequence Label: pLenti sgRNA(MS2) mCherry
FEATURES             Location/Qualifiers
     source          1..10334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..332
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      183..318
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     245..264
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        380..427
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             446..820
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    950..1071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(987..1006)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1041..1060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1120..1136)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1120..1136)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1133..1155)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1144..1160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1168..1174,1175..1192,1193..1198))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1213..1234
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1351..1368)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1522..2110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1602..1621)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2281..3072,3073..3141))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2904..2923
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3142..3246)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3321..3340)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3512..3891
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3893..4091
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4046..4066
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4109..4289
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4336..4461
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4954..5187
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5372..5416
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5714..5831
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5881..6129
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5881..5901
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6052..6071
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        6168..6186
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        6238..6256
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        6380..7558
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6611..7549
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7506..7526
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7565..8275
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7710..7728)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7978..7997
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    8300..8888
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8353..8373)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8771..8782)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8891..8907)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8892..8908)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9413..9593
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9619..9636)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9625..9849
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9895..10323
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9982..10001)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10192..10213
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(join(10332..10334,1..18))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
       61 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
      121 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
      181 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
      241 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
      301 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
      361 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
      421 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
      481 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
      541 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
      601 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
      661 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
      721 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
      781 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
      841 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
      901 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
      961 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     1021 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     1081 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     1141 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     1201 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     1261 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     1321 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1381 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1441 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1501 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1561 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1621 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1681 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1741 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1801 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1861 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1921 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1981 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2041 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2101 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2161 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2221 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2281 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2341 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     2401 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     2461 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2521 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     2581 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     2641 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2701 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     2761 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     2821 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     2881 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     2941 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3001 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3061 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3121 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3181 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3241 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     3301 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     3361 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     3421 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     3481 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
     3541 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     3601 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     3661 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     3721 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     3781 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     3841 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     3901 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     3961 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     4021 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     4081 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     4141 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     4201 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     4261 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     4321 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     4381 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     4441 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     4501 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     4561 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     4621 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     4681 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     4741 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     4801 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     4861 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     4921 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     4981 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     5041 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     5101 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     5161 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     5221 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     5281 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     5341 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     5401 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     5461 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     5521 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     5581 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     5641 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     5701 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     5761 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     5821 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta attagctagc
     5881 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
     5941 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
     6001 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
     6061 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     6121 cgaaacaccg ccaggaccgc gcttcccacg gttttagagc taggccaaca tgaggatcac
     6181 ccatgtctgc agggcctagc aagttaaaat aaggctagtc cgttatcaac ttggccaaca
     6241 tgaggatcac ccatgtctgc agggccaagt ggcaccgagt cggtgctttt tttggatcct
     6301 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     6361 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     6421 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     6481 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     6541 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     6601 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     6661 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     6721 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     6781 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     6841 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     6901 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     6961 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     7021 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     7081 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     7141 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     7201 agggagctca aaatgaagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     7261 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     7321 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     7381 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     7441 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     7501 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgatg
     7561 taccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     7621 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     7681 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     7741 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     7801 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     7861 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     7921 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     7981 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     8041 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     8101 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     8161 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     8221 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtgagaatt
     8281 cgatatcaag cttatcggta atcaacctct ggattacaaa atttgtgaaa gattgactgg
     8341 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     8401 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     8461 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     8521 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     8581 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     8641 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     8701 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     8761 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     8821 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     8881 ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag
     8941 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     9001 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     9061 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     9121 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     9181 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     9241 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     9301 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     9361 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     9421 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     9481 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     9541 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg
     9601 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     9661 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     9721 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     9781 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     9841 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     9901 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     9961 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
    10021 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
    10081 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
    10141 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
    10201 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
    10261 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
    10321 attaattctg tgga
//
