LOCUS       sequence-345203-       10451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mALG8 (ITYTWTRL) antigen as a fusion to luciferase and Cre
            recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Burger ML, Cruz AM, Crossland GE, Gaglia G, Ritch CC, Blatt SE, 
            Bhutkar A, Canner D, Kienka T, Tavana SZ, Barandiaran AL, Garmilla 
            A, Schenkel JM, Hillman M, de Los Rios Kobara I, Li A, Jaeger AM, 
            Hwang WL, Westcott PMK, Manos MP, Holovatska MM, Hodi FS, Regev A, 
            Santagata S, Jacks T
  TITLE     Antigen dominance hierarchies shape TCF1(+) progenitor CD8 T cell 
            phenotypes in tumors.
  JOURNAL   Cell. 2021 Sep 16;184(19):4996-5014.e26. doi: 
            10.1016/j.cell.2021.08.020.
  PUBMED    34534464
REFERENCE   2  (bases 1 to 10451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.08"; journalName: "Cell"; date: "2021-09-16- 
            16"; volume: "184"; issue: "19"; pages: "4996-5014.e26"
COMMENT     Sequence Label: Lenti-LucA
FEATURES             Location/Qualifiers
     source          1..10451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..200
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             218..398
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    445..570
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1067..1300
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1485..1529
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1827..1944
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2085..3296
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3133..3152
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3343..4992
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3409..3427)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        5091..5590
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5126..5149)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5523..5542
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5621..5641
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5642..6670
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    6723..7311
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6776..6796)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7194..7205)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7314..7330)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7315..7331)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7840..8020
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8082..8670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8162..8181)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8841..9632,9633..9701))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9464..9483
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9702..9806)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9881..9900)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10072..10451
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
      241 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      301 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      361 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      421 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      481 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      541 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      601 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      661 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      721 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
      781 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
      841 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
      901 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
      961 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1021 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1081 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1141 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1201 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1261 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1321 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1381 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1441 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1501 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1561 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     1621 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     1681 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     1741 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     1801 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     1861 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     1921 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     1981 ttagtaccgg gcccgggtcg actagtgatc agtgtgaggg agtgtaaagc tggtttcaga
     2041 gatccagttt ggttagtacc gggcccgctc tagacggttg atctggcctc cgcgccgggt
     2101 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
     2161 gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
     2221 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
     2281 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
     2341 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
     2401 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
     2461 ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg
     2521 gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg
     2581 ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct
     2641 gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg
     2701 tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag
     2761 ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca
     2821 ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg
     2881 ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc
     2941 tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc
     3001 gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc
     3061 tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc
     3121 ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt
     3181 gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt
     3241 gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacgaag
     3301 ctaacgcgct agctaactcg aacgctagct gtgcgatcgt ttatggaaga cgccaaaaac
     3361 ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg
     3421 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat
     3481 atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct
     3541 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt
     3601 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac
     3661 gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg
     3721 ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc
     3781 caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg
     3841 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc agagtccttc
     3901 gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg tctgcctaaa
     3961 ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag agatcctatt
     4021 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt
     4081 tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat
     4141 agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca aagtgcgctg
     4201 ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa atacgattta
     4261 tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt cggggaagcg
     4321 gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac tgagactaca
     4381 tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt
     4441 ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcaa
     4501 agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa caatccggaa
     4561 gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg
     4621 gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa gtacaaaggc
     4681 tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa catcttcgac
     4741 gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt
     4801 ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta
     4861 acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt
     4921 accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga
     4981 aagatcgccg tgtgtgcagt gggcatcaca tacacctgga ccaggctgta tgcttcagtg
     5041 ttgactggct aaaaaccgct gttcctagga atcccgaggc ctattctacc gggtagggga
     5101 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     5161 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     5221 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     5281 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     5341 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     5401 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     5461 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     5521 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     5581 gggagacccg acattagcgc tacagcttaa gcggaccatg cccaagaaga agaggaaggt
     5641 gtccaattta ctgaccgtac accaaaattt gcctgcatta ccggtcgatg caacgagtga
     5701 tgaggttcgc aagaacctga tggacatgtt cagggatcgc caggcgtttt ctgagcatac
     5761 ctggaaaatg cttctgtccg tttgccggtc gtgggcggca tggtgcaagt tgaataaccg
     5821 gaaatggttt cccgcagaac ctgaagatgt tcgcgattat cttctatatc ttcaggcgcg
     5881 cggtctggca gtaaaaacta tccagcaaca tttgggccag ctaaacatgc ttcatcgtcg
     5941 gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca ctggttatgc ggcggatccg
     6001 aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct ctagcgttcg aacgcactga
     6061 tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc tgccaggata tacgtaatct
     6121 ggcatttctg gggattgctt ataacaccct gttacgtata gccgaaattg ccaggatcag
     6181 ggttaaagat atctcacgta ctgacggtgg gagaatgtta atccatattg gcagaacgaa
     6241 aacgctggtt agcaccgcag gtgtagagaa ggcacttagc ctgggggtaa ctaaactggt
     6301 cgagcgatgg atttccgtct ctggtgtagc tgatgatccg aataactacc tgttttgccg
     6361 ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc cagctatcaa ctcgcgccct
     6421 ggaagggatt tttgaagcaa ctcatcgatt gatttacggc gctaaggatg actctggtca
     6481 gagatacctg gcctggtctg gacacagtgc ccgtgtcgga gccgcgcgag atatggcccg
     6541 cgctggagtt tcaataccgg agatcatgca agctggtggc tggaccaatg taaatattgt
     6601 catgaactat atccgtaacc tggatagtga aacaggggca atggtgcgcc tgctggaaga
     6661 tggcgattag aaacgttgtt gtttggggtt gaattactct tctagaccgc ggcgcgcccg
     6721 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6781 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6841 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6901 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6961 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     7021 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7081 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     7141 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7201 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7261 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     7321 gtcgacctcg atcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     7381 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     7441 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7501 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     7561 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     7621 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     7681 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     7741 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     7801 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     7861 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7921 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7981 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatgtgagc aaaaggccag
     8041 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8101 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8161 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8221 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8281 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8341 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8401 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8461 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8521 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8581 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8641 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8701 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8761 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8821 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8881 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8941 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9001 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9061 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9121 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9181 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9241 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9301 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9361 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9421 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9481 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9541 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9601 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9661 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9721 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9781 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     9841 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     9901 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     9961 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
    10021 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
    10081 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
    10141 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
    10201 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
    10261 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
    10321 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
    10381 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
    10441 ctattaccat g
//
