LOCUS       sequence-345204-       10511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mALG8 (ITYTWTRL) and mLAMA4 (VGFNFRTL) antigens as a 
            fusion to luciferase and Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10511)
  AUTHORS   Burger ML, Cruz AM, Crossland GE, Gaglia G, Ritch CC, Blatt SE, 
            Bhutkar A, Canner D, Kienka T, Tavana SZ, Barandiaran AL, Garmilla 
            A, Schenkel JM, Hillman M, de Los Rios Kobara I, Li A, Jaeger AM, 
            Hwang WL, Westcott PMK, Manos MP, Holovatska MM, Hodi FS, Regev A, 
            Santagata S, Jacks T
  TITLE     Antigen dominance hierarchies shape TCF1(+) progenitor CD8 T cell 
            phenotypes in tumors.
  JOURNAL   Cell. 2021 Sep 16;184(19):4996-5014.e26. doi: 
            10.1016/j.cell.2021.08.020.
  PUBMED    34534464
REFERENCE   2  (bases 1 to 10511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.08"; journalName: "Cell"; date: "2021-09-16- 
            16"; volume: "184"; issue: "19"; pages: "4996-5014.e26"
COMMENT     Sequence Label: Lenti-LucAL
FEATURES             Location/Qualifiers
     source          1..10511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     189..208
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             287..307
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             308..1336
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    1389..1977
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1442..1462)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1860..1871)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1980..1996)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1981..1997)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2506..2686
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(2748..3336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2828..2847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3507..4298,4299..4367))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4130..4149
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4368..4472)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4547..4566)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4738..5117
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5119..5317
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5272..5292
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5335..5515
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5562..5687
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6184..6417
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6602..6646
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6944..7061
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7202..8413
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     8250..8269
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             8460..10109
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(8526..8544)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        join(10268..10511,1..256)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10303..10326)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
       61 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
      121 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
      181 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
      241 atctccggga gacccgacat tagcgctaca gcttaagcgg accatgccca agaagaagag
      301 gaaggtgtcc aatttactga ccgtacacca aaatttgcct gcattaccgg tcgatgcaac
      361 gagtgatgag gttcgcaaga acctgatgga catgttcagg gatcgccagg cgttttctga
      421 gcatacctgg aaaatgcttc tgtccgtttg ccggtcgtgg gcggcatggt gcaagttgaa
      481 taaccggaaa tggtttcccg cagaacctga agatgttcgc gattatcttc tatatcttca
      541 ggcgcgcggt ctggcagtaa aaactatcca gcaacatttg ggccagctaa acatgcttca
      601 tcgtcggtcc gggctgccac gaccaagtga cagcaatgct gtttcactgg ttatgcggcg
      661 gatccgaaaa gaaaacgttg atgccggtga acgtgcaaaa caggctctag cgttcgaacg
      721 cactgatttc gaccaggttc gttcactcat ggaaaatagc gatcgctgcc aggatatacg
      781 taatctggca tttctgggga ttgcttataa caccctgtta cgtatagccg aaattgccag
      841 gatcagggtt aaagatatct cacgtactga cggtgggaga atgttaatcc atattggcag
      901 aacgaaaacg ctggttagca ccgcaggtgt agagaaggca cttagcctgg gggtaactaa
      961 actggtcgag cgatggattt ccgtctctgg tgtagctgat gatccgaata actacctgtt
     1021 ttgccgggtc agaaaaaatg gtgttgccgc gccatctgcc accagccagc tatcaactcg
     1081 cgccctggaa gggatttttg aagcaactca tcgattgatt tacggcgcta aggatgactc
     1141 tggtcagaga tacctggcct ggtctggaca cagtgcccgt gtcggagccg cgcgagatat
     1201 ggcccgcgct ggagtttcaa taccggagat catgcaagct ggtggctgga ccaatgtaaa
     1261 tattgtcatg aactatatcc gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct
     1321 ggaagatggc gattagaaac gttgttgttt ggggttgaat tactcttcta gaccgcggcg
     1381 cgcccgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     1441 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     1501 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     1561 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     1621 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     1681 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     1741 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     1801 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     1861 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     1921 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     1981 gataccgtcg acctcgatcg agacctagaa aaacatggag caatcacaag tagcaataca
     2041 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     2101 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     2161 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     2221 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     2281 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     2341 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     2401 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     2461 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     2521 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2581 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2641 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat gtgagcaaaa
     2701 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2761 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2821 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2881 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2941 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3001 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3061 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3121 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3181 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3241 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3301 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3361 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3421 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3481 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3541 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3601 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3661 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3721 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3781 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3841 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3901 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3961 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4021 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4081 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4141 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4201 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4261 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4321 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4381 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4441 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     4501 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     4561 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     4621 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     4681 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     4741 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     4801 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     4861 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     4921 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     4981 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     5041 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     5101 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     5161 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     5221 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     5281 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
     5341 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5401 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5461 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     5521 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     5581 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     5641 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     5701 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     5761 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     5821 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     5881 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     5941 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     6001 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     6061 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     6121 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     6181 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     6241 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     6301 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     6361 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     6421 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     6481 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     6541 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     6601 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     6661 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     6721 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     6781 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     6841 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     6901 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     6961 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     7021 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     7081 gcagagatcc agtttggtta gtaccgggcc cgggtcgact agtgatcagt gtgagggagt
     7141 gtaaagctgg tttcagagat ccagtttggt tagtaccggg cccgctctag acggttgatc
     7201 tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct
     7261 gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca
     7321 gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta
     7381 ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag
     7441 gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga
     7501 tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg
     7561 tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc
     7621 tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg
     7681 ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc
     7741 gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg
     7801 aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct
     7861 tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc
     7921 tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg ggggcggcag
     7981 ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt
     8041 cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt
     8101 gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa
     8161 tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc
     8221 ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg
     8281 gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc
     8341 aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc
     8401 ttttttgtta gacgaagcta acgcgctagc taactcgaac gctagctgtg cgatcgttta
     8461 tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa
     8521 ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg
     8581 cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg
     8641 ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat
     8701 gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg
     8761 cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt
     8821 cgcagcctac cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa
     8881 aaaagctccc aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat
     8941 ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt
     9001 ttgtgccaga gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat
     9061 ctactggtct gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc
     9121 atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg
     9181 ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc
     9241 gagtcgtctt aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca
     9301 agattcaaag tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga
     9361 ttgacaaata cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta
     9421 aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg
     9481 ggctcactga gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg
     9541 cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa
     9601 cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt
     9661 atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg
     9721 gagacatagc ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc
     9781 tgattaagta caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac
     9841 accccaacat cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc
     9901 ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt
     9961 acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg
    10021 aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa
    10081 aggccaagaa gggcggaaag atcgccgtgt gtgcagtggg catcacatac acctggacca
    10141 ggctgtatgc ttcagtgttg actggcttct ttgatggctt tgaagtaggc ttcaatttcc
    10201 gaacattaca gccaaatggg ttactataaa aaccgctgtt cctaggaatc ccgaggccta
    10261 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
    10321 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
    10381 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
    10441 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
    10501 gtagcacgtc t
//
