LOCUS       sequence-34521-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral transfer plasmid for Tet-regulated shRNA expression with 
            a TREtight promoter. Contains TurboRFP and Venus fluorescent 
            reporters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Zuber J, McJunkin K, Fellmann C, Dow LE, Taylor MJ, Hannon GJ, Lowe 
            SW
  TITLE     Toolkit for evaluating genes required for proliferation and survival
            using tetracycline-regulated RNAi.
  JOURNAL   Nat Biotechnol. 2011 Jan . 29(1):79-83.
  PUBMED    21131983
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jan . 29(1):79-83."
COMMENT     Sequence Label: TtTMPV-Neo
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..368
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    62..80
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    98..116
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    133..151
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    169..187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    205..223
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    240..258
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    276..294
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     341..365
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 cctcactcct tctctaggcg ccggaattga agatctgggg gatcgatacc gtcgagttta
       61 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacga
      121 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
      181 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat
      241 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
      301 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
      361 agatcgcctg gagaattaat tcgagctcgg tacccgggga tccaccatga gcgagctgat
      421 caaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa
      481 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga agatcaaggt
      541 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg
      601 cagcaaagcc ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc
      661 tgagggcttc acatgggaga gaatcaccac atacgaagac gggggcgtgc tgaccgctac
      721 ccaggacacc agcttccaga acggctgcat catctacaac gtcaagatca acggggtgaa
      781 cttcccatcc aacggccctg tgatgcagaa gaaaacacgc ggctgggagg ccaacaccga
      841 gatgct
//
