LOCUS       sequence-345212-        8835 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon vector for expression of RNAPII Ser2ph mintbody.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8835)
  AUTHORS   Ohishi H, Shimada S, Uchino S, Li J, Sato Y, Shintani M, Owada H, 
            Ohkawa Y, Pertsinidis A, Yamamoto T, Kimura H, Ochiai H
  TITLE     STREAMING-tag system reveals spatiotemporal relationships between 
            transcriptional regulatory factors and transcriptional activity.
  JOURNAL   Nat Commun. 2022 Dec 20;13(1):7672. doi: 10.1038/s41467-022-35286-2.
  PUBMED    36539402
REFERENCE   2  (bases 1 to 8835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-20"; volume: "13(1):7672. doi"; pages: " 
            10.1038/s41467-022-35286-2"
COMMENT     Sequence Label: pLR5-CAG-42B3mutAC2(Ser2ph)-SNAP
FEATURES             Location/Qualifiers
     source          1..8835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1054..1357
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1637..2653
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2577..2599
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2661..2680
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        2751..2769
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3626..3635
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3655..4200
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     intron          4311..4540
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4523..4542
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(4613..5187,5188..5190,5191..5199)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4780..4797)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5007..5026
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5222..6025
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5285..5304)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5895..5914
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6155..6276
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6192..6211)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   6534..6596
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6647..6667)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6647..6665)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6686..6702)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6686..6702)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6699..6721)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6710..6726
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6734..6740,6741..6758,6759..6764))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6779..6800
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6917..6934)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7088..7676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7168..7187)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7847..8638,8639..8707))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8470..8489
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8708..8812)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagttat taatagtaat caattacggg
     1021 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1081 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1141 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1201 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1261 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1321 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
     1381 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     1441 attattttgt gcagcgatgg gggcgggggg gggggggggg ggggcgccgg gggggggggg
     1501 gggggggggg ggggggggsg gggsgrggcg gagaggtgcg gcggcagcca atcagagcgg
     1561 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
     1621 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
     1681 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     1741 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
     1801 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
     1861 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
     1921 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     1981 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     2041 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     2101 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     2161 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     2221 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
     2281 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     2341 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     2401 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     2461 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     2521 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     2581 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
     2641 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
     2701 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
     2761 cactataggg agacccaagc ttggtatcga cctcggatcg tcgctagcga tatcatgggc
     2821 gccgaagtgc agctggtgga gtctggggga ggcttagtga agcctggagg gtccctgaaa
     2881 ctctcctgtg cagcctctgg attcactttc agtagctttg ccatgtcttg ggttcgccag
     2941 actccggaga agaggctgga gtgggtcgca accattagta gaagtggtag ttacacctac
     3001 tattcagaca gtgtgaaggg tcgattcacc atctccagag acaatgccaa gaacaccctg
     3061 tacctgcaaa tgagcagtct gaggtctgag gacacggccg tctattactg tgcaagagac
     3121 cccttcgatg tctggggcgc agggaccacg gtcaccgtct cctcaggtgg aggcggttca
     3181 ggcggaggtg gctctggcgg tggcggatcg caggctgttg tgactcagga atctgcactc
     3241 accacatcac ctggtgaaac agtcacactc acttgtcgct caagtactgg ggctgttaca
     3301 actagtaact atgccaactg ggtccaagaa aaaccagatc atttattcac tggtctaata
     3361 ggtggtacca acaaccgagc tccaggtgtt cctgccagat tctcaggctc cctgattgga
     3421 gacaaggctg ccctcaccat cacaggggca cagactgagg atgaggcaat atatttctgt
     3481 gctctatggt acagcaacct ttgggtgttc ggtggaggaa ccaaactgac tgtcctaggc
     3541 cagcccaagg cggatccaga tcccgccacc attgtacaag agcggcctga ggagcagagc
     3601 ccaggcgagc aacagcgccg tggacgccac catgggcgat cgcatgaatt caccatggac
     3661 aaagactgcg aaatgaagcg caccaccctg gatagccctc tgggcaagct ggaactgtct
     3721 gggtgcgaac agggcctgca ccgtatcatc ttcctgggca aaggaacatc tgccgccgac
     3781 gccgtggaag tgcctgcccc agccgccgtg ctgggcggac cagagccact gatgcaggcc
     3841 accgcctggc tcaacgccta ctttcaccag cctgaggcca tcgaggagtt ccctgtgcca
     3901 gccctgcacc acccagtgtt ccagcaggag agctttaccc gccaggtgct gtggaaactg
     3961 ctgaaagtgg tgaagttcgg agaggtcatc agctacagcc acctggccgc cctggccggc
     4021 aatcccgccg ccaccgccgc cgtgaaaacc gccctgagcg gaaatcccgt gcccattctg
     4081 atcccctgcc accgggtggt gcagggcgac ctggacgtgg ggggctacga gggcgggctc
     4141 gccgtgaaag agtggctgct ggcccacgag ggccacagac tgggcaagcc tgggctgggt
     4201 cctgcaggcg gatccgcgtt taaactcgag gttaattaat gagcggccgc atagataact
     4261 gatccagtgt gctggaatta attcgctgtc tgcgagggcc agctgttggg gtgagtactc
     4321 cctctcaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa acgaggagga
     4381 tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcgtcca tctggtcaga
     4441 aaagacaatc tttttgttgt caagcttgag gtgtggcagg cttgagatct ggccatacac
     4501 ttgagtgaca atgacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa
     4561 ctgcaggtcg agcatgcatc tagggcggcc aattccgccc ctctcccccc cccccctctc
     4621 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     4681 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     4741 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     4801 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     4861 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     4921 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     4981 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     5041 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     5101 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     5161 ttttcctttg aaaaacacga tgataagctt gccacaaccc gggataattc ctgcagccaa
     5221 tatgggatcg gccattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5281 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5341 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5401 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5461 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5521 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5581 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5641 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5701 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     5761 catgcccgac ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5821 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5881 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5941 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6001 tcgccttctt gacgagttct tctgagggga tcaattctct agataactga tcataatcag
     6061 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     6121 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     6181 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6241 tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtcgagtgca ttctagttgt
     6301 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac atactagtta
     6361 aaagttttgt tactttatag aagaaatttt gagtttttgt ttttttttaa taaataaata
     6421 aacataaata aattgtttgt tgaatttatt attagtatgt aagtgtaaat ataataaaac
     6481 ttaatatcta ttcaaattaa taaataaacc tcgatataca gaccgataaa acacatgcgt
     6541 caattttacg catgattatc tttaacgtac gtcacaatat gattatcttt ctagggttaa
     6601 tctagtatac gcgtatgcgg ccgcttaatt aatccagctt ttgttccctt tagtgagggt
     6661 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6721 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6781 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6841 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6901 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6961 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7021 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7081 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7141 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7201 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7261 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7321 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7381 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7441 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7501 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7561 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7621 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7681 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7741 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7801 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7861 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7921 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7981 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8041 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8101 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8161 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8221 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8281 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8341 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8401 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8461 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8521 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8581 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8641 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8701 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8761 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8821 cccgaaaagt gccac
//
