LOCUS       sequence-345215-        8832 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon vector for expression of RNAPII Ser5ph mintbody.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Ohishi H, Shimada S, Uchino S, Li J, Sato Y, Shintani M, Owada H, 
            Ohkawa Y, Pertsinidis A, Yamamoto T, Kimura H, Ochiai H
  TITLE     STREAMING-tag system reveals spatiotemporal relationships between 
            transcriptional regulatory factors and transcriptional activity.
  JOURNAL   Nat Commun. 2022 Dec 20;13(1):7672. doi: 10.1038/s41467-022-35286-2.
  PUBMED    36539402
REFERENCE   2  (bases 1 to 8832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-20"; volume: "13(1):7672. doi"; pages: " 
            10.1038/s41467-022-35286-2"
COMMENT     Sequence Label: pLR5-CAG-44B12mut23(Ser5ph)-SNAP
FEATURES             Location/Qualifiers
     source          1..8832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1054..1357
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1637..2653
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2577..2599
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2661..2680
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        2751..2769
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3623..3632
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3652..4197
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     intron          4308..4537
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4520..4539
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(4610..5184,5185..5187,5188..5196)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4777..4794)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5004..5023
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5219..6022
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5282..5301)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5892..5911
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6152..6273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6189..6208)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   6531..6593
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6644..6664)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6644..6662)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6683..6699)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6683..6699)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6696..6718)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6707..6723
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6731..6737,6738..6755,6756..6761))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6776..6797
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6914..6931)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7085..7673)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7165..7184)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7844..8635,8636..8704))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8467..8486
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8705..8809)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagttat taatagtaat caattacggg
     1021 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1081 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1141 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1201 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1261 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1321 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
     1381 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     1441 attattttgt gcagcgatgg gggcgggggg gggggggggg ggggcgccgg gggggggggg
     1501 gggggggggg ggggggggsg gggsgrggsg grgrggtgcg gcggcagcca atcagagcgg
     1561 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
     1621 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
     1681 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     1741 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
     1801 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
     1861 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
     1921 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     1981 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     2041 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     2101 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     2161 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     2221 ggtgccgggc ggggcggggc cgcctcgggc cggggrgggc tcgggggagg ggcgcggcgg
     2281 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     2341 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     2401 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     2461 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     2521 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     2581 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
     2641 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
     2701 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
     2761 cactataggg agacccaagc ttggtatcga cctcggatcg tcgctagcga tatcatgggc
     2821 gccgaggtcc agctgcaaca gtctggaccg gagctggtga agcctggggc ttcagtgaag
     2881 atatcctgca aggcttctgg atacacattc actgaataca ccatgcactg ggtgaaacag
     2941 agccatggac agagccttga gtggattgga ggcattaatc ctaacaatgt tcttacttac
     3001 tacaaccaga ggttcaaggg caaggccaca ttgactgtag acaagtcctc cagcacagcc
     3061 tacctggagc tccgcagcct gacatctgag gattctgcag tctattactg tgcaagaggc
     3121 acctcttttg cttactgggg ccaagggact ctggtcactg tctcctcagg tggaggcggt
     3181 tcaggcggag gtggctctgg cggtggcgga tcggatgttg tgatgaccca gactccactc
     3241 actttgtcgg ttaccattgg acaaccagcc tccatctctt gcaagtcaag tcagagcctc
     3301 ttatatagtg atggaaagac atatttgagt tggttgttac agaggccagg ccagtctcca
     3361 gagcgcctaa tctatctggt gtctaaactg gactctggag tccctgacag gttcactggc
     3421 agtggatcag ggacagattt cacactgaaa atcagcagag tggaggctga ggatttggga
     3481 gtttattatt gctggcaagg tacacatttt cctcggacgt tcggtggagg caccaagctg
     3541 gaaataaaac gggcggatcc cgccaccatt gtacaagagc ggcctgagga gcagagccca
     3601 ggcgagcaac agcgccgtgg acgccaccat gggcgatcgc atgaattcac catggacaaa
     3661 gactgcgaaa tgaagcgcac caccctggat agccctctgg gcaagctgga actgtctggg
     3721 tgcgaacagg gcctgcaccg tatcatcttc ctgggcaaag gaacatctgc cgccgacgcc
     3781 gtggaagtgc ctgccccagc cgccgtgctg ggcggaccag agccactgat gcaggccacc
     3841 gcctggctca acgcctactt tcaccagcct gaggccatcg aggagttccc tgtgccagcc
     3901 ctgcaccacc cagtgttcca gcaggagagc tttacccgcc aggtgctgtg gaaactgctg
     3961 aaagtggtga agttcggaga ggtcatcagc tacagccacc tggccgccct ggccggcaat
     4021 cccgccgcca ccgccgccgt gaaaaccgcc ctgagcggaa atcccgtgcc cattctgatc
     4081 ccctgccacc gggtggtgca gggcgacctg gacgtggggg gctacgaggg cgggctcgcc
     4141 gtgaaagagt ggctgctggc ccacgagggc cacagactgg gcaagcctgg gctgggtcct
     4201 gcaggcggat ccgcgtttaa actcgaggtt aattaatgag cggccgcata gataactgat
     4261 ccagtgtgct ggaattaatt cgctgtctgc gagggccagc tgttggggtg agtactccct
     4321 ctcaaaagcg ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg aggaggattt
     4381 gatattcacc tggcccgcgg tgatgccttt gagggtggcc gcgtccatct ggtcagaaaa
     4441 gacaatcttt ttgttgtcaa gcttgaggtg tggcaggctt gagatctggc catacacttg
     4501 agtgacaatg acatccactt tgcctttctc tccacaggtg tccactccca ggtccaactg
     4561 caggtcgagc atgcatctag ggcggccaat tccgcccctc tccccccccc ccctctccct
     4621 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     4681 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     4741 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     4801 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     4861 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     4921 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     4981 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     5041 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     5101 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     5161 tcctttgaaa aacacgatga taagcttgcc acaacccggg ataattcctg cagccaatat
     5221 gggatcggcc attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     5281 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     5341 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     5401 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5461 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5521 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     5581 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     5641 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     5701 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     5761 gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     5821 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     5881 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     5941 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     6001 ccttcttgac gagttcttct gaggggatca attctctaga taactgatca taatcagcca
     6061 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     6121 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     6181 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6241 ttgtggtttg tccaaactca tcaatgtatc ttaacgcgtc gagtgcattc tagttgtggt
     6301 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacata ctagttaaaa
     6361 gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa ataaataaac
     6421 ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata ataaaactta
     6481 atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca catgcgtcaa
     6541 ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta gggttaatct
     6601 agtatacgcg tatgcggccg cttaattaat ccagcttttg ttccctttag tgagggttaa
     6661 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6721 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6781 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6841 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6901 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6961 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7021 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7081 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7141 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7201 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7261 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7321 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7381 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7441 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7501 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7561 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7621 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7681 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7741 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7801 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7861 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7921 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7981 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8041 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8101 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8161 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8221 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8281 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8341 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8401 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8461 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8521 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8581 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8641 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8701 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8761 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8821 gaaaagtgcc ac
//
